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The map label for this gene is hycI [H]

Identifier: 218690841

GI number: 218690841

Start: 3105285

End: 3105755

Strand: Reverse

Name: hycI [H]

Synonym: ECED1_3168

Alternate gene names: 218690841

Gene position: 3105755-3105285 (Counterclockwise)

Preceding gene: 218690842

Following gene: 218690840

Centisome position: 59.62

GC content: 54.14

Gene sequence:

>471_bases
GTGACTGACGTTTTACTCTGTGTTGGCAATAGCATGATGGGCGATGATGGCGCAGGCCCGCTACTGGCGGAAAAGTGCGC
TGCCGCGCCGAAAGGTAACTGGGTAGTGATTGACGGCGGTAGCGCACCGGAAAACGACATTGTCGCTATTCGCGAACTGC
GCCCGACACGGCTGCTTATTGTCGACGCCACGGATATGGGGCTAAACCCTGGCGAGATCCGCATCATCGACCCGGCTGAC
ATCGCCGAGATGTTTATGATGACTACCCATAACATGCCGCTGAACTATCTTATCGACCAGTTGAAAGAAGATATTGGCGA
AGTGATTTTCCTTGGCATCCAGCCGGATATCGTCGGCTTTTACTACCCGATGACTCAGCAGATTAAAGATGCGGTGGAAA
CGGTGTATCAGCGGCTAGAAGGCTGGGAAGGAAATGGCGGCTTCGCGCAGTTAGCGGCGGAAGAAGAGTAG

Upstream 100 bases:

>100_bases
GCGCAAACTTCGCAGCAGAAGGAATGGAGTACAATGCTGTTAAGTATGCTGCATGATATTCATCAGGAAAACGCCATCTA
TTTGATGGTGAGGAGACTGC

Downstream 100 bases:

>100_bases
TTTTTCAGTAAGGAATTCAGGGAATTGAACATCAGGACAGGGATGTGCGCTTTTTTATTGAGCTTAGTGCTGCCTGCACA
GGCCACCAGCTTTACCGAAT

Product: hydrogenase 3 maturation protease

Products: NA

Alternate protein names: HycI protease [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPAD
IAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE

Sequences:

>Translated_156_residues
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPAD
IAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE
>Mature_155_residues
TDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPADI
AEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGFYYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE

Specific function: Protease involved in the C-terminal processing of hycE, the large subunit of hydrogenase 3 [H]

COG id: COG0680

COG function: function code C; Ni,Fe-hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A31 family [H]

Homologues:

Organism=Escherichia coli, GI1789072, Length=156, Percent_Identity=98.0769230769231, Blast_Score=310, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004420
- InterPro:   IPR000671 [H]

Pfam domain/function: PF01750 HycI [H]

EC number: =3.4.23.51 [H]

Molecular weight: Translated: 17016; Mature: 16885

Theoretical pI: Translated: 3.82; Mature: 3.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
5.2 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI
CCCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEE
VDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGF
EECCCCCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEE
YYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE
ECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC
>Mature Secondary Structure 
TDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPENDIVAIRELRPTRLLI
CCEEEEECCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEECCCEEEEE
VDATDMGLNPGEIRIIDPADIAEMFMMTTHNMPLNYLIDQLKEDIGEVIFLGIQPDIVGF
EECCCCCCCCCCEEEECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHEEEECCCCCCEEE
YYPMTQQIKDAVETVYQRLEGWEGNGGFAQLAAEEE
ECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]