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The map label for this gene is dam [C]

Identifier: 218690773

GI number: 218690773

Start: 3044554

End: 3045483

Strand: Reverse

Name: dam [C]

Synonym: ECED1_3098

Alternate gene names: 218690773

Gene position: 3045483-3044554 (Counterclockwise)

Preceding gene: 218690774

Following gene: 218690772

Centisome position: 58.46

GC content: 46.56

Gene sequence:

>930_bases
ATGGGTGATGCTATCAATTCGCTCATTAACCCCATTAGAAGCTTTAGGATATGTTATTGCAGGAGGTGCTGTATGAGCAC
CATCCTGAAATGGGCGGGTAATAAAACCGCCATTATGTCCGAACTGAAAAAACACCTTCCTGCAGGCCCGCGACTGGTTG
AACCTTTCGCGGGTTCTTGTGCTGTGATGATGGAGACGGATTACCCCAGCTATCTGGTTGCGGATATTAATCCTGATTTA
ATCAACCTCTATAAAAAGGTTGCCGCTGATTGTGAATCGTTTATATCTCGCGCCAGAGTTTTATTTGAGATCGCAAACAG
GGAGGTGGCTTATTACAACATAAGGCAGGAGTTTAATTACTCCACTGAAATTACTGATTTCATGAAAGCGGTATATTTCC
TGTATCTCAATCGTCACGGTTACCGTGGTTTATGTCGCTATAACAAGAGCGGGCATTTCAACATTCCCTACGGTAATTAT
AAAAATCCGTATTTCCCTGAAAAAGAACTTCGCACATTTGCAGAAAAAGCCCAGCGAGCAACGTTTATCTGCGCAAGCTT
TGATGAAACGCTGGCGATGCTGCAGGTGGGGGATGTGGTGTATTGCGATCCGCCGTATGACGGTACGTTTTCCGGCTATC
ACACTGATGGTTTCACTGAAGATGACCAGTATCACCTGGCATCCGTTCTTGAACATCGGTCATCAGAAGGACATCCGGTC
ATTGTTTCTAACAGTGATACATCCCTGATCCGTTCGCTGTATCGCAATTTTACTCACCACTACATCAAGGCAAAACGCAG
CATCGGTGTAGCAGCTGGTGAGAGTAAATCTGCAACAGAAATCATCGCTGTTTCCGGGCCGCGCTGCTGGGTGGGATTTG
ATCCTTCGCGTGGCGTGGATAGTTCTGCCGTGTACGGAGTGCGTGCATGA

Upstream 100 bases:

>100_bases
GGAGTCATTACCGGAAGCATGGCGTATTTACGGGTAGGGAATGGGCCGCGGTTATCTCTCATGTGCTTCCGGTTAATGAC
GTCATGGCAGTTTCAGAACA

Downstream 100 bases:

>100_bases
GCCATGCTGATATGAACAACTGCAGCGGCTTTAACGAGGTCGCCGCAGCATTCTCATGGAACAGCCCGAAAAAGGCCATT
AACCCTTATCTGGACCCGGC

Product: site-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MGDAINSLINPIRSFRICYCRRCCMSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDL
INLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNY
KNPYFPEKELRTFAEKAQRATFICASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPV
IVSNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVDSSAVYGVRA

Sequences:

>Translated_309_residues
MGDAINSLINPIRSFRICYCRRCCMSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDL
INLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNY
KNPYFPEKELRTFAEKAQRATFICASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPV
IVSNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVDSSAVYGVRA
>Mature_308_residues
GDAINSLINPIRSFRICYCRRCCMSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDYPSYLVADINPDLI
NLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYK
NPYFPEKELRTFAEKAQRATFICASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVI
VSNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVDSSAVYGVRA

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=268, Percent_Identity=46.6417910447761, Blast_Score=241, Evalue=5e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 34959; Mature: 34828

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDAINSLINPIRSFRICYCRRCCMSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSC
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCCCCE
AVMMETDYPSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNY
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHCCC
STEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRA
CHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
TFICASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPV
EEEEECHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEE
IVSNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVD
EEECCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEECCCEEEEECCCCCCCC
SSAVYGVRA
CCCCCCCCC
>Mature Secondary Structure 
GDAINSLINPIRSFRICYCRRCCMSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSC
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEECCCCCCE
AVMMETDYPSYLVADINPDLINLYKKVAADCESFISRARVLFEIANREVAYYNIRQEFNY
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHCCC
STEITDFMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKELRTFAEKAQRA
CHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
TFICASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPV
EEEEECHHHHHHHHHCCCEEEECCCCCCCCCCEECCCCCCCCHHHHHHHHHHCCCCCCEE
IVSNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRGVD
EEECCCHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCEEEEEECCCEEEEECCCCCCCC
SSAVYGVRA
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]