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The map label for this gene is nemA [H]

Identifier: 218689596

GI number: 218689596

Start: 1808542

End: 1809639

Strand: Direct

Name: nemA [H]

Synonym: ECED1_1850

Alternate gene names: 218689596

Gene position: 1808542-1809639 (Clockwise)

Preceding gene: 218689595

Following gene: 218689597

Centisome position: 34.72

GC content: 53.01

Gene sequence:

>1098_bases
ATGTCATCTGAAAAACTGTATTCCCCACTGAAAGTGGGCGCGATCACGGCGGCAAATCGTATTTTTATGGCACCGCTGAC
GCGTCTGCGCAGTATTGAACCGGGTGACATCCCTACCCCGTTGATGGCGGAATACTATCGCCAACGTGCCAGTGCCGGTT
TGATTATTAGCGAAGCCACGCAAATTTCTGCCCAGGCAAAAGGATACGCAGGTGCACCTGGCATCCATAGCCAGGAACAA
ATTGCCGCATGGAAAAAAATTACCGCTGGCGTTCATGCTGAAAATGGTCATATGGCCGTACAGCTGTGGCACACCGGACG
CATTTCTCACGCCAGCCTGCAACCTGGCGGTCAGGCACCGGTAGCGCCTTCAGCACTTAGCGCGGGAACACGTACTTCTC
TGCGCGATGAAAATGGTCAGGCGATCCGTGTTGAAACATCCATGCCGCGTGCGCTTGAACTGGGAGAGATTCAGGGTATC
GTCAATGATTTCCGTCAGGCCATTGCTAACGCGCGTGAAGCCGGTTTTGATCTGGTAGAGCTCCACTCTGCTCACGGTTA
TTTGCTGCATCAGTTCCTTTCCCCTTCTTCAAACCATCGTACCGATCAGTACGGTGGCAGCGTGGAAAATCGCGCGCGTC
TGGTGCTGGAAGTGGTCGATGCCGGGATTGAAGAATGGGGTGCCGATCGCATTGGCATTCGCATTTCGCCAATCGGTACT
TTCCAGAACACGGATAACGGCCCGAATGAAGAAGCCGATGCACTGTATCTGATTGAACAACTGGGTAAACGCGGCATTGC
TTATCTTCATATGTCAGAACCAGATTGGGCGGGAGGTGAACCATATACTGATGCGTTTCGCGAAAAAGTACGCGCCCGTT
TCCACGGTCCGATTATCGGCGCAGGTGCATACACAGTAGAAAAAGCTGAAACGCTGATCGGCAAAGGGTTAATTGATGCG
GTAGCATTTGGTCGTGACTGGATTGCGAACCCGGATCTGGTCGCCCGCTTGCAGCGCAAAGCTGAGCTTAACCCGCAGCG
TGCCGAAAGTTTTTATGGTGGCGGTGCTGAAGGTTACACTGATTACCCGACGCTGTAA

Upstream 100 bases:

>100_bases
TTTCGAGCCACTGGAAAACGCGCTGGCCCATGTAAAAAACATTATTGCGACGCCTGCCGTTTAGCAGGCATTTTTTATCA
CCAGACGACCGGGAGCCTTT

Downstream 100 bases:

>100_bases
TCCAACATTGCGAGCGGCGCAAAGCCGCCGCTATACTAAAACAACTTTTTGAATCTGTTAGCCATTTTGAGGATATAAAG
ATGCGTCTTCTTCATACCAT

Product: N-ethylmaleimide reductase

Products: NADP; Cytotoxic compound [C]

Alternate protein names: N-ethylmaleimide reducing enzyme [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIQGI
VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Sequences:

>Translated_365_residues
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQ
IAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIQGI
VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDA
VAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL
>Mature_364_residues
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEATQISAQAKGYAGAPGIHSQEQI
AAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIQGIV
NDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGTF
QNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAV
AFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYTDYPTL

Specific function: Catalyzes the reduction of N-ethylmaleimide (NEM) to N- ethylsuccinimide [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1787939, Length=365, Percent_Identity=98.9041095890411, Blast_Score=734, Evalue=0.0,
Organism=Escherichia coli, GI1789463, Length=359, Percent_Identity=31.1977715877437, Blast_Score=117, Evalue=9e-28,
Organism=Caenorhabditis elegans, GI17565138, Length=351, Percent_Identity=29.3447293447293, Blast_Score=104, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17559802, Length=351, Percent_Identity=28.7749287749288, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17540738, Length=374, Percent_Identity=28.6096256684492, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI72001454, Length=354, Percent_Identity=30.225988700565, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17564188, Length=351, Percent_Identity=26.7806267806268, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17559804, Length=268, Percent_Identity=29.4776119402985, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17566914, Length=216, Percent_Identity=30.5555555555556, Blast_Score=69, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6325086, Length=389, Percent_Identity=39.0745501285347, Blast_Score=237, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6321973, Length=389, Percent_Identity=37.2750642673522, Blast_Score=221, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001155 [H]

Pfam domain/function: PF00724 Oxidored_FMN [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 39521; Mature: 39390

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIQGIVNDFRQAIANAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC
>Mature Secondary Structure 
SSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAEYYRQRASAGLIISEAT
CCCHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCEEEEHHH
QISAQAKGYAGAPGIHSQEQIAAWKKITAGVHAENGHMAVQLWHTGRISHASLQPGGQAP
HHHHHCCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCEEEEEEECCCCCCCCCCCCCCCC
VAPSALSAGTRTSLRDENGQAIRVETSMPRALELGEIQGIVNDFRQAIANAREAGFDLVE
CCCHHHCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEVVDAGIEEWGADRIGIRISPIGT
HHCCCHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
FQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDWAGGEPYTDAFREKVRARFHGPIIG
CCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEE
AGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLVARLQRKAELNPQRAESFYGGGAEGYT
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHCCCCCCCCC
DYPTL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH; N-ethylmaleimide [C]

Specific reaction: NADPH + N-ethylmaleimide = NADP + Cytotoxic compound [C]

General reaction: Reduction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9013822; 9278503 [H]