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The map label for this gene is folM

Identifier: 218689552

GI number: 218689552

Start: 1763128

End: 1763850

Strand: Direct

Name: folM

Synonym: ECED1_1805

Alternate gene names: 218689552

Gene position: 1763128-1763850 (Clockwise)

Preceding gene: 218689551

Following gene: 218689554

Centisome position: 33.84

GC content: 51.18

Gene sequence:

>723_bases
ATGGGTAAAACCCAGTCCTTGCCAATATTAATTACTGGCGGAGGTCGCCGCATCGGCCTCGCCCTCGCATGGCATTTCAT
TAATCAAAAGCAACCGGTGATTGTCAGCTATCGGACGCACTATCCCGCCATTGATGGACTGATTAAGGCAGGTGCGCAGT
GTATTCAGGCTGATTTTTCGACCAACGACGGTGTGATGGCGTTTGCCGATGAAGTACTAAAAAGCACCCATGGTCTGCGT
GCTATTTTGCATAACGCCAGCGCGTGGATGGCGGAAAAACCGGGAGCGCCACTGACCGACGTACTGGCTTGCATGATGCA
GATCCACGTTAATACCCCATACCTGCTCAACCATGCTCTGGAAAGATTACTGCGTGGGCACGGACACGCCGCCAGCGATA
TCATTCACTTTACCGATTATGTGGTGGAGCGCGGTAGCGACAAACATATTGCGTATGCTGCAAGCAAAGCGGCACTGGAT
AATATGACCCGCTCGTTTGCCCGCAAGCTGGCACCGGAAGTGAAAGTGAATTCTATTGCGCCATCGCTGATCCTGTTTAA
TGAACATGATGATGCTGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACAGCGCCTGGCGAGAAAGAAGTGA
TCGACCTGGTCGATTACTTACTTACCAGCTGCTTTGTCACCGGACGCAGTCTCCCACTTGATGGCGGTCGTCATCTGCGT
TAA

Upstream 100 bases:

>100_bases
AATGGTGCTGATTGGCATGCTGTTAATTGCCTCAGTTCCGGCGACCTGGATGCTGGTGGGATAACGTTCATCCCATCGTT
GGATAGCAAAGGAGATAACG

Downstream 100 bases:

>100_bases
TGCAGCTTAATCCAGCATATAATCAACACCCATGCGCTTATTCCCCAGCACGCAACAGAGCTAACAAAAGCAGCGGGCAA
GCGCATCATTCCGGTGAAAT

Product: short chain dehydrogenase

Products: NA

Alternate protein names: DHFR

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLR
AILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR

Sequences:

>Translated_240_residues
MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLR
AILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR
>Mature_239_residues
GKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRA
ILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDN
MTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR

Specific function: Catalyzes the reduction of dihydrofolate to tetrahydrofolate

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FolM subfamily

Homologues:

Organism=Escherichia coli, GI1787891, Length=240, Percent_Identity=98.3333333333333, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI2367175, Length=244, Percent_Identity=25.8196721311475, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLM_ECOL6 (Q8FHB1)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_753893.1
- ProteinModelPortal:   Q8FHB1
- SMR:   Q8FHB1
- EnsemblBacteria:   EBESCT00000041793
- GeneID:   1036028
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c1998
- NMPDR:   fig|199310.1.peg.1936
- GeneTree:   EBGT00050000009188
- HOGENOM:   HBG750976
- OMA:   RAIIHNA
- ProtClustDB:   PRK06483
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PRINTS:   PR00081

Pfam domain/function: PF00106 adh_short

EC number: =1.5.1.3

Molecular weight: Translated: 26349; Mature: 26217

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00061 ADH_SHORT

Important sites: ACT_SITE 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLIKAGAQCIQADFS
CCCCCCCCEEEECCCCEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
GKTQSLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAIDGLIKAGAQCIQADFS
CCCCCCCEEEECCCCEEHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCC
TNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGAPLTDVLACMMQIHVNTPYLLNHAL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHH
ERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALDNMTRSFARKLAPEVKVNSIA
HHHHHCCCCHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCEECCCC
PSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVDYLLTSCFVTGRSLPLDGGRHLR
CEEEEEECCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157