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The map label for this gene is mcbR [H]

Identifier: 218689377

GI number: 218689377

Start: 1583314

End: 1583550

Strand: Direct

Name: mcbR [H]

Synonym: ECED1_1603

Alternate gene names: 218689377

Gene position: 1583314-1583550 (Clockwise)

Preceding gene: 218689374

Following gene: 218689379

Centisome position: 30.39

GC content: 47.68

Gene sequence:

>237_bases
ATGTTGGGTTCTGTCCTTGCGTCCGCATGGCGCGCCGGTTCCTTACGTCTGGTTTCGGTAAATGCGCTCTTTGTCGCACC
AGCGCAAGCATTTACAGTTCCGGAAGTGGGAAAACGTCAATTAAATGAAATCAATCGGATCCGCTACGAACTGGAATTAA
TGGCAGTTGCTCTGGCTGTCGAAAACCTCACTATGCAAAAAAACGTTGCTTTGTTGTGCCAACAATACAGCCGATAA

Upstream 100 bases:

>100_bases
GGCTGCAAACAAAACCAATCCTGTGAGCCTGCTACACTATTGATGGATTCATGCCAAAAGCACAGGAGAGCATTATGGGG
CAACAAACGCAGCGTAACCG

Downstream 100 bases:

>100_bases
ATAAAATACGCCCGGCAAATACCGGGCTTAAAGATAATTACTCAAATCGCCACGCAATATTTATGCCGACACCATAATTT
CGTCCGGGCGCAGGTTCGTA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 78; Mature: 78

Protein sequence:

>78_residues
MLGSVLASAWRAGSLRLVSVNALFVAPAQAFTVPEVGKRQLNEINRIRYELELMAVALAVENLTMQKNVALLCQQYSR

Sequences:

>Translated_78_residues
MLGSVLASAWRAGSLRLVSVNALFVAPAQAFTVPEVGKRQLNEINRIRYELELMAVALAVENLTMQKNVALLCQQYSR
>Mature_78_residues
MLGSVLASAWRAGSLRLVSVNALFVAPAQAFTVPEVGKRQLNEINRIRYELELMAVALAVENLTMQKNVALLCQQYSR

Specific function: Important for biofilm formation. Represses expression of mcbA by binding to its promoter region, which prevents colanic acid overproduction and mucoidy [H]

COG id: COG1802

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081914, Length=57, Percent_Identity=87.719298245614, Blast_Score=97, Evalue=2e-22,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 8633; Mature: 8633

Theoretical pI: Translated: 9.94; Mature: 9.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLGSVLASAWRAGSLRLVSVNALFVAPAQAFTVPEVGKRQLNEINRIRYELELMAVALAV
CCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLTMQKNVALLCQQYSR
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLGSVLASAWRAGSLRLVSVNALFVAPAQAFTVPEVGKRQLNEINRIRYELELMAVALAV
CCHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
ENLTMQKNVALLCQQYSR
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]