Click here to switch to the map view.

The map label for this gene is ogt

Identifier: 218689324

GI number: 218689324

Start: 1517832

End: 1518347

Strand: Reverse

Name: ogt

Synonym: ECED1_1545

Alternate gene names: 218689324

Gene position: 1518347-1517832 (Counterclockwise)

Preceding gene: 218689325

Following gene: 218689323

Centisome position: 29.15

GC content: 52.91

Gene sequence:

>516_bases
ATGCTGAGATTACTTGAAGAAAAAATTGCCACGCCACTGGGTCCACTGTGGGTGATTTGCGATGAGCAATTTCGCCTGCG
GGCGGTTGAATGGGAAGAGTACAGCGAACGCATGGTGCAGCTGCTGGACATCCATTACCGCAAAGAAGGCTATGAACGTA
TTTCTGCCACCAACCCAGGTGGTTTAAGTGACAAGCTTCGTGAATATTTTGCCGGTAATCTTAGCATTATTGATACGCTT
CCCACTGCCACGGGGGGGACGCCATTTCAGCGCGAAGTCTGGAAAACATTACGCACAATCCCCTGCGGGCAGGTAATGCA
TTACGGCCAACTGGCTGAACAATTGGGCCGCCCCGGTGCGGCGCGTGCCGTAGGTGCGGCAAACGGATCGAATCCCATCA
GCATCGTCGTACCTTGCCATCGGGTTATTGGCCGAAACGGCACCATGACCGGATATGCAGGCGGAGTTCAGCGAAAAGAG
TGGTTATTGCGCCACGAAGGTTATCTTTTGCTGTAA

Upstream 100 bases:

>100_bases
ATCTTTTTGGTGGTATGGCTGCTGATGTTGCTGGCGTGGTATCTTGTTGGCTTGCCGATAGGTCCGGGTATTTACCCACG
TTTGTCTTAAGAGAGAACGG

Downstream 100 bases:

>100_bases
ACATTAAACAATTTGTGCCAGCTTGTTCACACTTTTATGTAAAGTCACCCTTAACAACTTAAGGGTTTTCAAATAGATAG
ACATATATTTACATCTAATA

Product: O-6-alkylguanine-DNA:cysteine-protein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Sequences:

>Translated_171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL
>Mature_171_residues
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTL
PTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
WLLRHEGYLLL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family

Homologues:

Organism=Homo sapiens, GI197304670, Length=83, Percent_Identity=50.6024096385542, Blast_Score=87, Evalue=8e-18,
Organism=Escherichia coli, GI1787596, Length=171, Percent_Identity=100, Blast_Score=352, Evalue=9e-99,
Organism=Escherichia coli, GI1788542, Length=89, Percent_Identity=51.685393258427, Blast_Score=104, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115533068, Length=93, Percent_Identity=38.7096774193548, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=93, Percent_Identity=38.7096774193548, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=48.1481481481481, Blast_Score=90, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137554, Length=105, Percent_Identity=41.9047619047619, Blast_Score=68, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OGT_ECOLI (P0AFH0)

Other databases:

- EMBL:   Y00495
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   B64883
- RefSeq:   AP_001963.1
- RefSeq:   NP_415851.1
- ProteinModelPortal:   P0AFH0
- SMR:   P0AFH0
- DIP:   DIP-48073N
- MINT:   MINT-1243838
- STRING:   P0AFH0
- PRIDE:   P0AFH0
- EnsemblBacteria:   EBESCT00000003089
- EnsemblBacteria:   EBESCT00000017511
- GeneID:   945853
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW1329
- KEGG:   eco:b1335
- EchoBASE:   EB0662
- EcoGene:   EG10668
- eggNOG:   COG0350
- GeneTree:   EBGT00050000009234
- HOGENOM:   HBG752398
- OMA:   NLRAVEW
- ProtClustDB:   PRK10286
- BioCyc:   EcoCyc:EG10668-MONOMER
- BioCyc:   MetaCyc:EG10668-MONOMER
- Genevestigator:   P0AFH0
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR10815
- TIGRFAMs:   TIGR00589

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N; SSF46767 MethylDNA_cys_mtrans_DNA_bd

EC number: =2.1.1.63

Molecular weight: Translated: 19179; Mature: 19179

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00374 MGMT

Important sites: ACT_SITE 139-139

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
CCCHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGA
CCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
HHEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC
>Mature Secondary Structure
MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDIHYRKEGYERISATNPG
CCCHHHHHHCCCCCCEEEEECCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPCGQVMHYGQLAEQLGRPGA
CCHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCC
ARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKEWLLRHEGYLLL
HHEECCCCCCCCEEEEEECHHHHCCCCCCCCCCCCCHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330