Click here to switch to the map view.

The map label for this gene is ycjW [H]

Identifier: 218689310

GI number: 218689310

Start: 1501608

End: 1502606

Strand: Reverse

Name: ycjW [H]

Synonym: ECED1_1528

Alternate gene names: 218689310

Gene position: 1502606-1501608 (Counterclockwise)

Preceding gene: 218689314

Following gene: 218689293

Centisome position: 28.84

GC content: 45.95

Gene sequence:

>999_bases
ATGTCCCCTACTATTTATGATATTGCCAGGGTTGCAGGCGTATCAAAATCCACCGTATCACGCGTGCTGAATAAGCAAAC
CAATATCTCCCCGGAAGCGCGCGAAAAAGTGTTACGGGCCATTGAAGAATTACAGTATCAACCAAACAAGCTGGCCCGCG
CGCTGACCTCTTCGGGTTTTGATGCCATTATGGTGATTTCTACCCGTTCGACCAAAACTACGGCGGGTAATCCATTTTTC
TCGGAAGTTTTGCATGCCATCACCGCCAAAGCAGAAGAAGAAGGTTTCGACGTGATATTACAAACGTCGCACAATCCGGC
AGAAGATCTGCAAAAATGCGAAAGCAAAATTAAGCAGAAAATGATTAAAGGCATTATTATGCTAAGTTCGCCTGCCGATG
AATCCTTCTTTGCACAACTCGACAAATACGAGATCCCGGTGGTGGTGATCGGTAAAGTTGAAGGTCAATACAGCCATGTT
TATTCCGTCGATACCGATAATTATGGCGACAGTATTGCGCTGACCAATGCGTTAATTGAAAGCGGGCATAAGAATATAGC
CTGCCTGCATGCGCCGCTTGATGTACATGTTTCAGTGGATCGGGTAAATGGTTATAAGCAAAGCCTGGCTGCGCATAATA
TTGCAGTGCGTGATGAATGGATTGTTGACGGCGGTTATACCCATGAAACAGCCTTGCAAGCCGCACGGCAATTATTAAGC
CAGTCGCCGTTGCCAGAAGCCGTATTTGCCACTGACAGCCTGAAATTAATGAGCATTTATCGTGCAGCAGCAGAGAAAAA
TATTGCTATTCCGCAGCAGTTAGCGGTGGTGGGTTATAGTAATGAAACGCTGTCGTTTATATTAACGCCTGCACCAGGCG
GCATCGATGTTCCGACGCAGGAGTTAGGTCAACAAAGCTGCGAGTTATTATTCCAGCTAATTGCCGGAAAACCGTCACCA
CAAAATATTACCGTTGCTACGCATATGTCGTTGAAATAA

Upstream 100 bases:

>100_bases
TGTGGGGGCCAGGCCATTGATTATCAACTACCCGTGTTGTCATCGACGGACGAACTCCATAAGATGTGCAGCATTCACGT
TCGGACGGAGTTCCTGACCT

Downstream 100 bases:

>100_bases
ATATATGCCGGATTTGGCGCTCGCCCCGCATCCGGCAATTTAGCACATTATCAGAACGAGTAATTCACCCCAACACCCGC
ATAATGGAATTTATCGCTGT

Product: putative DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSP
QNITVATHMSLK

Sequences:

>Translated_332_residues
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFF
SEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHV
YSVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSP
QNITVATHMSLK
>Mature_331_residues
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFS
EVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHVY
SVDTDNYGDSIALTNALIESGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLSQ
SPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFQLIAGKPSPQ
NITVATHMSLK

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787580, Length=332, Percent_Identity=97.289156626506, Blast_Score=672, Evalue=0.0,
Organism=Escherichia coli, GI1787948, Length=324, Percent_Identity=30.8641975308642, Blast_Score=161, Evalue=6e-41,
Organism=Escherichia coli, GI1790194, Length=312, Percent_Identity=30.4487179487179, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=29.1021671826625, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=27.8481012658228, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1788474, Length=320, Percent_Identity=28.75, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994940, Length=317, Percent_Identity=25.2365930599369, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1789202, Length=320, Percent_Identity=27.1875, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787906, Length=324, Percent_Identity=25.9259259259259, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786540, Length=328, Percent_Identity=25.609756097561, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1786268, Length=289, Percent_Identity=23.8754325259516, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790689, Length=336, Percent_Identity=27.6785714285714, Blast_Score=81, Evalue=9e-17,
Organism=Escherichia coli, GI1790715, Length=320, Percent_Identity=21.25, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36122; Mature: 35990

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHVYSVDTDNYGDSIALTNALIE
HHHCEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHH
SGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFQLIAGKPSPQNITVATHMSLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC
>Mature Secondary Structure 
SPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEELQYQPNKLARALTSSGF
CCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCC
DAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQTSHNPAEDLQKCESKIKQK
CEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHH
MIKGIIMLSSPADESFFAQLDKYEIPVVVIGKVEGQYSHVYSVDTDNYGDSIALTNALIE
HHHCEEEECCCCCHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHH
SGHKNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRDEWIVDGGYTHETALQAARQLLS
CCCCCEEEEECCCEEEEEEEHHCCHHHHHHHCCEEECCEEEECCCCCHHHHHHHHHHHHH
QSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAVVGYSNETLSFILTPAPGGIDVPTQ
CCCCCCHHHCCCCHHHHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEEECCCCCCCCCHH
ELGQQSCELLFQLIAGKPSPQNITVATHMSLK
HHHHHHHHHHHHHHCCCCCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]