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The map label for this gene is narX [H]

Identifier: 218689170

GI number: 218689170

Start: 1357153

End: 1358949

Strand: Reverse

Name: narX [H]

Synonym: ECED1_1371

Alternate gene names: 218689170

Gene position: 1358949-1357153 (Counterclockwise)

Preceding gene: 218689178

Following gene: 218689169

Centisome position: 26.09

GC content: 53.09

Gene sequence:

>1797_bases
ATGCTTAAACGTTGTCTCTCTCCGCTCACCCTGGTTAATCAGGTTGCGCTTATTGTGTTGCTTTCTACTGCTATTGGACT
GGCAGGGATGGCGGTTTCTGGCTGGCTGGTGCAAGGCGTTCAGGGCAGCGCCCATGCGATCAACAAAGCGGGATCGCTGC
GCATGCAAAGTTACCGTCTGTTGGCAGCAGTGCCATTAAGCGAGAAAGACAAGCCCTTAATTAAAGAGATGGAACAAACA
GCATTTAGCGCCGAGTTGACTCGAGCAGCAGAACGAGACGGGCAACTGGCGCAATTACAGGGTTTACAAGATTACTGGCG
TAATGAACTGATCCCTGCACTGATACGTGCACAAAACCGCGAAACGGTGTCAGCGGATGTCAGCCAGTTTGTTGCCGGGC
TTGATCAGCTGGTATCTGGTTTTGATCGCACCACGGAAATGCGCATTGAGACAGTGGTGCTGGTCCATCGGGTAATGGCG
GTATTTATGGCACTTTTACTGGTATTCACTATTATCTGGTTGCGGGCGAGATTGCTACAACCGTGGCGGCAACTGCTGGC
AATGGCGAGTGCCGTCAGCCATCGCGATTTTACCCAACGCGCAAACATCAGCGGGCGCAACGAAATGGCGATGCTTGGAA
CTGCGTTGAACAATATGTCTGCAGAACTGGCGGAAAGTTATGCCGTACTTGAGCAGCGGGTTCAGGAGAAAACCGCCGGG
CTGGAGCATAAAAATCAGATCCTCTCTTTTTTATGGCAGGCTAACCGCCGTTTGCATTCCCGCGCCCCGCTGTGTGAACG
CCTGTCACCTGTACTCAACGACTTACAGAATTTAACCCTGCTACGTGATATCGAACTGCGGGTGTATGACACTGATGATG
AAGAGAATCATCAGGAGTTTACCTGCCAGTCAGATATGACTTGTGATGATAAAGGCTGCCAGCTCTGCCCGCGCGGCGTA
TTACCCGTTGGCGATCGCGGCACAACCCTGAAGTGGCGGCTGGCTGACACTCATACGCAGTACGGTATTTTGCTGGCGAC
CCTGCCGCAGGGGCGTCATCTTAGCCATGATCAACAACAACTGGTGGATACCCTGGTTGAACAACTCACCGCCACGCTGG
CGCTGGATCGCCATCAGGAACGTCAGCAACAGTTGATCGTGATGGAAGAGCGTGCCACCATTGCGCGCGAACTGCATGAT
TCTATTGCCCAATCTCTCTCTTGCATGAAGATGCAGGTGAGTTGCTTACAGATGCAGGGCGATGCGCTGCCAGAAAGCAG
CCGCGAACTGTTAAGTCAGATCCGTAACGAACTGAATGCATCCTGGGTGCAGTTGCGTGAATTGCTCACCACATTCCGTT
TGCAGCTCACCGAGCCTGGATTACGTCCGGCGCTGGAGGCGAGTTGCGAAGAGTACAGCGCCAAATTTGGCTTCCCGGTG
AAGCTGGATTATCAATTGCCGCCTCGTCTGGTGCCTTCGCATCAGGCAATCCACTTGTTGCAAATTGCCCGTGAGGCATT
AAGTAACGCCCTCAAACATTCGCAAGCGAGTGAGGTCGTGGTGACGGTGGCGCAAAACGATAATCAGGTCAAACTGACCG
TCCAGGATAACGGCTGCGGCGTGCCTGAAAATGCCATCCGCAGCAATCACTACGGCATGATAATAATGCGCGACCGTGCG
CAAAGTTTACGAGGCGATTGCCGCGTCCGCCGTCGTGAATCAGGTGGCACCGAAGTGGTGGTCACCTTTATTCCCGAAAA
AACTTTCACAGACGTCCAAGGAGATACCCATGAGTAA

Upstream 100 bases:

>100_bases
GGTATACTCCAGCCAATAGCCGAGAATACTGCCATTCCAGAATGTATCGTCACATTCATTAAGGTTATTGCTCATTTAAA
GCCTGAAGGAAGAGGTTTAC

Downstream 100 bases:

>100_bases
TCAGGAACCGGCTACTATCCTGCTGATCGACGATCACCCGATGTTGCGAACTGGCGTAAAACAGCTTATCAGTATGGCAC
CAGATATCACCGTGGTTGGC

Product: nitrate/nitrite sensor protein NarX

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQT
AFSAELTRAAERDGQLAQLQGLQDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQNLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGV
LPVGDRGTTLKWRLADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRA
QSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE

Sequences:

>Translated_598_residues
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQT
AFSAELTRAAERDGQLAQLQGLQDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQNLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGV
LPVGDRGTTLKWRLADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRA
QSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE
>Mature_598_residues
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQT
AFSAELTRAAERDGQLAQLQGLQDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
LEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQNLTLLRDIELRVYDTDDEENHQEFTCQSDMTCDDKGCQLCPRGV
LPVGDRGTTLKWRLADTHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHD
SIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWVQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRA
QSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE

Specific function: Acts as a sensor for nitrate/nitrite and transduces signal of nitrate availability to the narL protein and of both nitrate/nitrite to the narP protein. NarX probably activates narL and narP by phosphorylation in the presence of nitrate. NarX also plays a

COG id: COG3850

COG function: function code T; Signal transduction histidine kinase, nitrate/nitrite-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1787474, Length=598, Percent_Identity=99.1638795986622, Blast_Score=1216, Evalue=0.0,
Organism=Escherichia coli, GI1788812, Length=588, Percent_Identity=29.421768707483, Blast_Score=197, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR016380
- InterPro:   IPR003661
- InterPro:   IPR011712
- InterPro:   IPR005467 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF07730 HisKA_3 [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 67157; Mature: 67157

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALIRAQNR
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQ
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQNLTLLRDIELRVYDTDDEENHQEF
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCE
TCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRLADTHTQYGILLATLPQGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPESSRELLSQIRNELNASWVQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCC
VPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE
CCHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSAHAINKAGSLRMQSYRL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHE
LAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGLQDYWRNELIPALIRAQNR
EEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
ETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMAVFMALLLVFTIIWLRARLLQ
CHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNNMSAELAESYAVLEQRVQEKTAG
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LEHKNQILSFLWQANRRLHSRAPLCERLSPVLNDLQNLTLLRDIELRVYDTDDEENHQEF
CHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCE
TCQSDMTCDDKGCQLCPRGVLPVGDRGTTLKWRLADTHTQYGILLATLPQGRHLSHDQQQ
EECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCCCCHHHH
LVDTLVEQLTATLALDRHQERQQQLIVMEERATIARELHDSIAQSLSCMKMQVSCLQMQG
HHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DALPESSRELLSQIRNELNASWVQLRELLTTFRLQLTEPGLRPALEASCEEYSAKFGFPV
CCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCE
KLDYQLPPRLVPSHQAIHLLQIAREALSNALKHSQASEVVVTVAQNDNQVKLTVQDNGCG
EECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEECCCCC
VPENAIRSNHYGMIIMRDRAQSLRGDCRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE
CCHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]