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The map label for this gene is purB [H]

Identifier: 218689084

GI number: 218689084

Start: 1276601

End: 1277971

Strand: Reverse

Name: purB [H]

Synonym: ECED1_1275

Alternate gene names: 218689084

Gene position: 1277971-1276601 (Counterclockwise)

Preceding gene: 218689085

Following gene: 218689083

Centisome position: 24.53

GC content: 52.52

Gene sequence:

>1371_bases
ATGGAATTATCCTCACTGACCGCCGTTTCCCCTGTCGATGGACGCTACGGCGATAAAGTCAGCGCACTGCGCGGGATTTT
CAGCGAATACGGTTTGCTGAAATTCCGTGTACAAGTTGAAGTACGTTGGCTGCAAAAACTGGCCGCGCACGCAGCGATCA
AGGAAGTTCCTGCTTTTGCTGCCGACGCAATCGGTTACCTTGATGCAATTGTCGCCAGTTTCAGCGAAGAAGATGCAGCA
CGCATCAAAACCATCGAGCGTACCACTAACCACGACGTTAAAGCGGTTGAGTATTTCCTGAAAGAAAAAGTGGCGGATAT
CCCGGAACTGCACGCGGTTTCTGAATTCATCCACTTTGCCTGTACTTCGGAAGATATCAATAACCTCTCCCACGCATTAA
TGCTGAAAACCGCGCGTGATGAAGTGATCCTGCCGTACTGGCGTCAACTGATTGATGGCATTAAAGATCTCGCCGTTCAG
TATCGCGATATCCCGCTGCTGTCTCGTACCCACGGTCAGCCAGCCACGCCGTCAACCATCGGTAAAGAGATGGCTAATGT
CGCCTACCGTATGGAGCGCCAGTACCGCCAGCTTAATCAGGTGGAGATCCTCGGCAAAATCAACGGCGCTGTCGGTAACT
ACAACGCCCACATCGCCGCTTACCCGGAAGTTGACTGGCATCAGTTCAGCGAAGAGTTCGTCACCTCGCTGGGTATTCAG
TGGAACCCGTACACCACCCAGATCGAACCGCACGACTACATTGCCGAACTGTTTGATTGCGTTGCGCGCTTCAACACCAT
TCTGATCGACTTTGACCGTGACGTCTGGGGTTATATCGCCCTTAACCACTTCAAACAGAAAACCATTGCTGGTGAGATTG
GTTCTTCCACCATGCCGCATAAAGTTAACCCGATCGACTTCGAAAACTCCGAAGGGAACCTGGGCCTTTCCAACGCGGTA
TTGCAGCACCTGGCAAGCAAACTGCCAGTTTCCCGCTGGCAGCGTGACCTGACCGACTCCACCGTGCTGCGTAACCTCGG
CGTGGGTATCGGTTATGCGCTGATTGCGTATCAATCCACCCTGAAAGGCGTGAGCAAACTGGAAGTGAACCGTGACCATC
TACTGGATGAACTGGATCACAACTGGGAAGTGCTGGCTGAACCAATCCAGACAGTTATGCGTCGCTATGGCATCGAAAAA
CCGTACGAGAAGCTGAAAGAGCTGACTCGCGGTAAGCGCGTTGACGCCGAAGGCATGAAGCAGTTTATCGATGGTCTGGC
GTTGCCAGAAGAAGAGAAAGCCCGCCTGAAAGCGATGACGCCGGCTAACTACATTGGTCGCGCCATCACCATGGTTGATG
AGCTGAAATAA

Upstream 100 bases:

>100_bases
CCAGGTCGGCGGCGGTCGTCTGCAACTGATGTTTTCTCGTAATCGCCTGACCACTCAGGCAAAACAAATTCTTGCTCATT
TAACCCCGGAGTTGTGATCT

Downstream 100 bases:

>100_bases
GCCTCAAAACAGTGCCGGATGGCGATGCTGTCCGGCCTGCTTATTAAGATTATCCACTTTTTGTTTTTTCACTTTACCTC
CCCTTCCCGCTGGTTTATTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK

Sequences:

>Translated_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
>Mature_456_residues
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFAADAIGYLDAIVASFSEEDAA
RIKTIERTTNHDVKAVEYFLKEKVADIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAV
LQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEK
PYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=99.780701754386, Blast_Score=943, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51529; Mature: 51529

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKEKVADIPELHAVSEFIHFA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTI
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHH
GKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWLQKLAAHAAIKEVPAFA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHH
ADAIGYLDAIVASFSEEDAARIKTIERTTNHDVKAVEYFLKEKVADIPELHAVSEFIHFA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
CTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQYRDIPLLSRTHGQPATPSTI
CCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHH
GKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHIAAYPEVDWHQFSEEFVTSLGIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCC
WNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVWGYIALNHFKQKTIAGEIGSSTMPH
CCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQRDLTDSTVLRNLGVGIGYALIAYQST
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKGVSKLEVNRDHLLDELDHNWEVLAEPIQTVMRRYGIEKPYEKLKELTRGKRVDAEGMK
HHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHH
QFIDGLALPEEEKARLKAMTPANYIGRAITMVDELK
HHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]