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The map label for this gene is hyaF [H]

Identifier: 218688873

GI number: 218688873

Start: 1079970

End: 1080827

Strand: Direct

Name: hyaF [H]

Synonym: ECED1_1061

Alternate gene names: 218688873

Gene position: 1079970-1080827 (Clockwise)

Preceding gene: 218688872

Following gene: 218688874

Centisome position: 20.73

GC content: 54.66

Gene sequence:

>858_bases
ATGAGCGAAACTTTTTTCCATCTGCTGGGGCCAGGAACGCAACCGAACGATGACAGTTTCAGCATGAATCCACTGCCGAT
CACCTGTCGGGTGAATGGTGAACCGAGTATGGCTGCCCTGGAGCAATGTGCTCACAGCCCGCAGGTGATTGCGCTGTTAA
ACGAGTTACAACATCAACTAAGCGAACGCCAGCCGCCGCTGGGTGAGGTACTGGCAGTCGATCTGTTAAATCTCAACGCC
GACGATCGTCACTTTATCAATACGCTTCTCGGGGAAGGGGAAGTGTCAGTGCGCATTCAGCAGGCTAACGACAGTGAAAG
TGAAATTCAGGAGGCGATCTTCTGCGGATTATGGCGGGTGCGCAGACGTCACGGCGAACAGTTGCTGGAAGACAAACTGG
AGGCTGGCTGCGCACCGCTGGCGTTGTGGCAGGCGGCAACGCAAAACGTCTTGCCGACAGATTCGCTGTTACCGCCGCCC
ATTGATGGCCTGATGAATGGCCTACCGTTGGCCCATGAATTACTGGCACATGTACGTAACCCCGACGCGCAGCCGCACAG
CATTAATCTGATTCAATTACCCATCAGCGAAGCTGACCGGCTTTTTCTCTCACGTCTCTGTGGGCCGGGAAATATTCAGA
TTCGTACCATTGGCTATGGCGAGAGCTATATCAACGCCACGGGGTTACGCCATGTCTGGCATTTACGCTGTACGGACACC
TTAAAAGGCCCGTTACTGGAAAGTTATGAAATCTGCCCAATACCGGAAGTGGTGCTGGCAGCGCCAGAAGATTTGGTCGA
CTCTGCGCAGCGGCTTAGCGAGGTATGCCAGTGGCTGGCGGAGGGTGCACCGACGTAA

Upstream 100 bases:

>100_bases
CCGGCGGAAACTATCGCGGCGTGCTGAATGGTATTCACCCGTGGGCGGAACTGATAAACCTGATGCGCGGGCTTGTCGAA
CCGCAACAGGAGCGTGCTTC

Downstream 100 bases:

>100_bases
AAAGACGGTAAGTATCGCTTTCAGGCTTATGAATATCGCAATCGGCGAATACCTCTGGTCGTAGAGTTTCAGGATAAAGA
GGGAGATCTACCATTAACGG

Product: protein involved in nickel incorporation into hydrogenase-1 proteins

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNA
DDRHFINTLLGEGEVSVRIQQANDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLIQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT

Sequences:

>Translated_285_residues
MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNA
DDRHFINTLLGEGEVSVRIQQANDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPP
IDGLMNGLPLAHELLAHVRNPDAQPHSINLIQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT
>Mature_284_residues
SETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCAHSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNAD
DRHFINTLLGEGEVSVRIQQANDSESEIQEAIFCGLWRVRRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPPI
DGLMNGLPLAHELLAHVRNPDAQPHSINLIQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDTL
KGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT

Specific function: Not known. Could enhance the incorporation of nickel to the hydrogenase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupH/hyaF family [H]

Homologues:

Organism=Escherichia coli, GI1787211, Length=285, Percent_Identity=97.1929824561404, Blast_Score=563, Evalue=1e-162,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006894 [H]

Pfam domain/function: PF04809 HupH_C [H]

EC number: NA

Molecular weight: Translated: 31365; Mature: 31234

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCAHSPQVIALLNELQHQL
CCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQANDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLIQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT
HCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SETFFHLLGPGTQPNDDSFSMNPLPITCRVNGEPSMAALEQCAHSPQVIALLNELQHQL
CCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHH
SERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQANDSESEIQEAIFCGLWRV
HHCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
RRRHGEQLLEDKLEAGCAPLALWQAATQNVLPTDSLLPPPIDGLMNGLPLAHELLAHVRN
HHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHCC
PDAQPHSINLIQLPISEADRLFLSRLCGPGNIQIRTIGYGESYINATGLRHVWHLRCTDT
CCCCCCEEEEEEECCCHHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHHHHEEEEEECHH
LKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVCQWLAEGAPT
HCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2180913; 8905232; 9278503 [H]