Definition Escherichia coli ED1a chromosome, complete genome.
Accession NC_011745
Length 5,209,548

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The map label for this gene is ydfQ [H]

Identifier: 218688840

GI number: 218688840

Start: 1050567

End: 1051100

Strand: Direct

Name: ydfQ [H]

Synonym: ECED1_1028

Alternate gene names: 218688840

Gene position: 1050567-1051100 (Clockwise)

Preceding gene: 218688838

Following gene: 218688841

Centisome position: 20.17

GC content: 54.31

Gene sequence:

>534_bases
ATGAACGCAAAAATCAAATACGGCCTGTCGGCTGCCGTTCTGGCGCTGATTGCCGCAGGGGCTTCTGCGCCTGAAATTCT
CGACCAGTTTCTGGATGAAAAGGAAGGTAACCACACCACGGCATACCGTGATGGTGCAGGTATCTGGACCATCTGCCGTG
GTGCCATCATGGTGGATGGCAAACCTGTCGTTCCGGGCATGAAGTTGTCGAAGGAAAAATGCGACCAGGTTAACGCCATT
GAGCGTGATAAGGCGCTGGCGTGGGTGGAGAAAAACATCAGAGTGCCGCTGACCGAACCCCAGAAAGCGGGGATCGCGTC
ATTCTGTCCGTACAACATTGGTCCCGGTAAGTGTTTCCCGTCGACGTTTTACAGACGAATTAATGCTGGTGATCGCAGGG
GAGCATGCGAAGCGATTCGCTGGTGGATTAAGGACGGTGGCAGAGACTGCCGTATTCGCTCAAATAACTGTTACGGTCAG
GTATTCCGTCGTGACCAGGAGAGCGCGCTGGCGTGCTGGGGAATCGACAGATAA

Upstream 100 bases:

>100_bases
TGTTTATCAGATTGACATACCCTGGCCAGTGCCCACCACTGGCGGGGTGAAGGCTTAACATATCCAGGGATTCGGAACCG
ATAAATCCTGATAAATATCC

Downstream 100 bases:

>100_bases
GCAGAATATTTTGCTGAAAAATGCGGTTTGCTCACACGGACGGATAACACGAAATCCTGCGAACTGACAAAAACTAAGTG
AATAAAAGTAAAAACCCCGT

Product: putative membrane-associated lysozyme; Qin prophage

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAI
ERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
VFRRDQESALACWGIDR

Sequences:

>Translated_177_residues
MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAI
ERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
VFRRDQESALACWGIDR
>Mature_177_residues
MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDGKPVVPGMKLSKEKCDQVNAI
ERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
VFRRDQESALACWGIDR

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1787836, Length=177, Percent_Identity=88.7005649717514, Blast_Score=335, Evalue=1e-93,
Organism=Escherichia coli, GI1786768, Length=143, Percent_Identity=30.7692307692308, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 19583; Mature: 19583

Theoretical pI: Translated: 8.93; Mature: 8.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDG
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCEEEECC
KPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFP
CCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
STFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVFRRDQESALACWGIDR
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MNAKIKYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRDGAGIWTICRGAIMVDG
CCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCEEEECCCCHHHHHCCEEEECC
KPVVPGMKLSKEKCDQVNAIERDKALAWVEKNIRVPLTEPQKAGIASFCPYNIGPGKCFP
CCCCCCCCCCHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
STFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQVFRRDQESALACWGIDR
HHHHHHHCCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]