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The map label for this gene is asnC

Identifier: 218688773

GI number: 218688773

Start: 991949

End: 993349

Strand: Reverse

Name: asnC

Synonym: ECED1_0960

Alternate gene names: 218688773

Gene position: 993349-991949 (Counterclockwise)

Preceding gene: 218688774

Following gene: 218688772

Centisome position: 19.07

GC content: 52.61

Gene sequence:

>1401_bases
ATGAGCGTTGTGCCTGTAGCCGACGTACTCCAGGGCCGCGTAGCCGTTGACAGCGAAGTCACCGTGCGCGGATGGGTACG
TACCCGCCGAGATTCAAAAGCTGGCATCTCCTTCCTCGCCGTTTATGACGGTTCCTGCTTTGATCCTGTACAGGCTGTCA
TCAATAATTCTCTGCCCAATTACAATGAAGACGTCCTGCGTCTGACCACCGGCTGCTCGGTCATTGTGACGGGTAAAGTC
GTGGCGTCGCCGGGCCAGGGGCAACAATTTGAAATTCAGGCCAGCAAGGTTGAAGTTGCTGGTTGGGTTGAAGATCCAGA
CACTTACCCGATGGCGGCAAAACGCCACAGCATTGAGTATCTGCGTGAAGTCGCTCACCTGCGTCCGCGCACAAACCTGA
TTGGTGCCGTCGCGCGCGTTCGCCATACGCTGGCGCAGGCGCTGCATCGCTTCTTTAACGAGCAGGGATTCTTCTGGGTT
TCAACGCCACTGATTACCGCATCTGATACCGAAGGTGCAGGCGAAATGTTCCGTGTTTCTACGCTGGATCTGGAAAACCT
GCCGCGTAACGATCAGGGCAAAGTGGATTTCGACAAAGACTTCTTTGGTAAAGAGTCTTTCCTGACCGTATCTGGCCAGT
TGAACGGCGAAACCTACGCTTGCGCATTGTCCAAAATTTATACCTTCGGCCCGACTTTCCGTGCTGAAAACTCCAACACC
AGCCGTCACCTGGCGGAATTCTGGATGCTGGAGCCGGAAGTGGCGTTTGCTAACCTGAACGATATCGCAGGTCTGGCTGA
AGCCATGCTGAAATATGTCTTCAAAGCGGTTCTCGAAGAACGCGCTGACGACATGAAATTCTTCGCTGAACGCGTAGATA
AAGATGCCGTTTCACGTCTGGAACGCTTCATTGAGGCCGATTTCGCGCAGGTGGATTACACCGACGCAGTGACCATTCTC
GAAAACTGCGGCAGGAAGTTTGAAAACCCGGTTTACTGGGGCGTCGATCTCTCTTCTGAGCATGAGCGTTATCTGGCGGA
AGAACACTTTAAAGCACCAGTAGTGGTTAAAAACTATCCGAAAGATATTAAAGCGTTCTATATGCGCCTTAACGAAGACG
GTAAAACCGTTGCGGCTATGGACGTTCTGGCTCCGGGCATCGGTGAGATCATTGGTGGCTCCCAGCGTGAAGAACGTCTG
GACGTGCTGGACGAGCGTATGCTTGAAATGGGCCTGAACAAAGAAGATTACTGGTGGTATCGCGATCTGCGTCGCTACGG
TACTGTTCCGCATTCAGGTTTCGGTCTTGGTTTTGAACGCCTGATTGCTTACGTAACTGGCGTGCAAAACGTACGTGATG
TTATTCCGTTCCCACGTACTCCGCGTAACGCCAGCTTCTAA

Upstream 100 bases:

>100_bases
ATTCTGCTTGTCTGATTGCAGATGCCAGGTAACATAGGTATCCCCCCATTTACGGGTGGACAAGTGTTTATTTTTTCCGA
CTATTAACAGAGAGAATATT

Downstream 100 bases:

>100_bases
TTTAGCGTCTTCAAGAGCCAGCGCCCGCTGGCTCTTTTTTTATCCTTTCTGTCAAGTTATCTGTTTGTTAAGTCAAGCAA
TCTATTTGCAACCCCGCCAT

Product: asparaginyl-tRNA synthetase

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS

Number of amino acids: Translated: 466; Mature: 465

Protein sequence:

>466_residues
MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKV
VASPGQGQQFEIQASKVEVAGWVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGETYACALSKIYTFGPTFRAENSNT
SRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKAVLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTIL
ENCGRKFENPVYWGVDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIGGSQREERL
DVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQNVRDVIPFPRTPRNASF

Sequences:

>Translated_466_residues
MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKV
VASPGQGQQFEIQASKVEVAGWVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGETYACALSKIYTFGPTFRAENSNT
SRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKAVLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTIL
ENCGRKFENPVYWGVDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIGGSQREERL
DVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQNVRDVIPFPRTPRNASF
>Mature_465_residues
SVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVV
ASPGQGQQFEIQASKVEVAGWVEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWVS
TPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGETYACALSKIYTFGPTFRAENSNTS
RHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKAVLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILE
NCGRKFENPVYWGVDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIGGSQREERLD
VLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQNVRDVIPFPRTPRNASF

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI226491502, Length=463, Percent_Identity=41.9006479481641, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI4758762, Length=455, Percent_Identity=28.1318681318681, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI45439306, Length=350, Percent_Identity=29.4285714285714, Blast_Score=129, Evalue=8e-30,
Organism=Escherichia coli, GI1787161, Length=466, Percent_Identity=100, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI1789256, Length=472, Percent_Identity=21.3983050847458, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788173, Length=111, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI71984122, Length=471, Percent_Identity=28.4501061571125, Blast_Score=188, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17556372, Length=433, Percent_Identity=31.1778290993072, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI17551876, Length=342, Percent_Identity=27.4853801169591, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319872, Length=470, Percent_Identity=36.3829787234043, Blast_Score=289, Evalue=5e-79,
Organism=Saccharomyces cerevisiae, GI6321807, Length=457, Percent_Identity=29.9781181619256, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323011, Length=487, Percent_Identity=24.435318275154, Blast_Score=116, Evalue=7e-27,
Organism=Drosophila melanogaster, GI19922416, Length=464, Percent_Identity=35.5603448275862, Blast_Score=278, Evalue=7e-75,
Organism=Drosophila melanogaster, GI19921528, Length=467, Percent_Identity=29.1220556745182, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI17136276, Length=490, Percent_Identity=26.734693877551, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): SYN_ECO24 (A7ZK21)

Other databases:

- EMBL:   CP000800
- RefSeq:   YP_001462150.1
- ProteinModelPortal:   A7ZK21
- SMR:   A7ZK21
- STRING:   A7ZK21
- EnsemblBacteria:   EBESCT00000023364
- GeneID:   5587779
- GenomeReviews:   CP000800_GR
- KEGG:   ecw:EcE24377A_1030
- eggNOG:   COG0017
- GeneTree:   EBGT00050000009271
- HOGENOM:   HBG745843
- OMA:   EPEMAFC
- ProtClustDB:   PRK03932
- BioCyc:   ECOL331111:ECE24377A_1030-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00534
- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR22594
- PRINTS:   PR01042
- TIGRFAMs:   TIGR00457

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti; SSF50249 Nucleic_acid_OB

EC number: =6.1.1.22

Molecular weight: Translated: 52571; Mature: 52440

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPN
CCCCCHHHHHCCCEECCCCEEEHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCC
YNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGWVEDPDTYPMAAKRHSIEY
CCCHHHEEECCCEEEEECEEEECCCCCCEEEEEECEEEEEEECCCCCCCCCHHHHHHHHH
LREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWVSTPLITASDTEGAGEMFRVS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCEEEEE
TLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGETYACALSKIYTFGPTFRAENSNT
EECHHHCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEECCCCCC
SRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKAVLEERADDMKFFAERVDKDAVSRL
HHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ERFIEADFAQVDYTDAVTILENCGRKFENPVYWGVDLSSEHERYLAEEHFKAPVVVKNYP
HHHHHHHHHHCCHHHHHHHHHHCCHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCC
KDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIGGSQREERLDVLDERMLEMGLNKEDYWWY
HHHHHHHHHCCCCCCEEEHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
RDLRRYGTVPHSGFGLGFERLIAYVTGVQNVRDVIPFPRTPRNASF
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDGSCFDPVQAVINNSLPN
CCCCHHHHHCCCEECCCCEEEHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCC
YNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGWVEDPDTYPMAAKRHSIEY
CCCHHHEEECCCEEEEECEEEECCCCCCEEEEEECEEEEEEECCCCCCCCCHHHHHHHHH
LREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWVSTPLITASDTEGAGEMFRVS
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEECCCCCCCCEEEEE
TLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGETYACALSKIYTFGPTFRAENSNT
EECHHHCCCCCCCCCCCCHHHCCCCCEEEEEECCCCCHHHHHHHHHHHCCCCEECCCCCC
SRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKAVLEERADDMKFFAERVDKDAVSRL
HHHHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHH
ERFIEADFAQVDYTDAVTILENCGRKFENPVYWGVDLSSEHERYLAEEHFKAPVVVKNYP
HHHHHHHHHHCCHHHHHHHHHHCCHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCC
KDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIGGSQREERLDVLDERMLEMGLNKEDYWWY
HHHHHHHHHCCCCCCEEEHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
RDLRRYGTVPHSGFGLGFERLIAYVTGVQNVRDVIPFPRTPRNASF
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA