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The map label for this gene is nagE [H]

Identifier: 218688485

GI number: 218688485

Start: 683283

End: 685229

Strand: Direct

Name: nagE [H]

Synonym: ECED1_0660

Alternate gene names: 218688485

Gene position: 683283-685229 (Clockwise)

Preceding gene: 218688479

Following gene: 218688486

Centisome position: 13.12

GC content: 54.39

Gene sequence:

>1947_bases
ATGAATATTTTAGGTTTTTTCCAGCGACTCGGTAGGGCGTTACAGCTCCCTATCGCGGTGCTGCCGGTGGCGGCGCTGTT
GCTGCGATTCGGTCAGCCAGATTTACTTAACGTTGCGTTTATTGCCCAGGCGGGCGGTGCGATTTTTGATAACCTCGCAT
TAATCTTCGCCATCGGCGTGGCATCCAGCTGGTCGAAAGACAGCGCAGGTGCGGCGGCATTGGCGGGTGCGGTAGGTTAC
TTTGTGTTAACCAAAGCGATGGTGACCATCAACCCAGAAATTAACATGGGTGTACTGGCGGGTATCATTACCGGTCTGGT
TGGTGGCGCAGCCTATAACCGTTGGTCCGATATTAAACTGCCGGACTTCCTGAGCTTCTTCGGCGGCAAACGCTTTGTGC
CGATCGCCACCGGCTTCTTCTGTCTGGTGCTGGCGGCCATTTTTGGTTACGTCTGGCCGCCGGTACAGCACGCTATCCAT
GCAGGCGGCGAGTGGATCGTTTCTGCGGGCGCGCTGGGTTCCGGTATCTTTGGTTTCATCAACCGTCTGCTGATCCCAAC
CGGTCTGCATCAGGTGCTGAACACCATCGCCTGGTTCCAGATTGGTGAATTCACCAACGCGGCGGGAACGGTTTTCCACG
GTGATATCAACCGCTTCTATGCCGGTGACGGCACCGCGGGGATGTTCATGTCCGGCTTCTTCCCGATTATGATGTTCGGT
CTGCCGGGTGCGGCGCTGGCGATGTACTTCGCAGCACCGAAAGAGCGTCGTCCGATGGTTGGCGGGATGCTGCTTTCTGT
TGCTGTTACTGCGTTCCTGACCGGTGTGACTGAGCCGCTGGAATTCCTGTTCATGTTCCTTGCACCGCTGCTGTACCTCC
TGCACGCACTGCTGACCGGTATCAGCCTGTTTGTGGCAACGTTGCTGGGTATCCATGCCGGCTTCTCTTTCTCTGCGGGG
GCTATCGACTACGCGTTGATGTATAATCTGCCGGCCGCCAGCCAGAACGTCTGGATGCTGCTGGTGATGGGGGTTGTCTT
CTTCGCTATCTACTTCGTGGTGTTCAGTTTGGTTATCCGCATGTTCAACCTGAAAACGCCGGGTCGTGAAGATAAAGAAG
ACGAGATCGTTACTGAAGAGGCTAACAGCAATACTGAAGAAGGTCTCAATCAACTGGCAACCAACTATATTGCTGCGGTT
GGCGGTACTGACAACCTGAAAGCCATTGATGCCTGTATTACTCGTCTGCGCCTTACCGTGGTTGACTCAGCTCGCGTCAA
CGATGCGATGTGTAAACGTCTGGGTGCTTCTGGGGTAGTGAAACTGAACAAACAGACTATTCAGGTGATTGTTGGCGCGA
AAGCAGAATCCATCGGCGATGCGATGAAGAAAGTCGTTGCCCGTGGTCCGGTAGCCGCTGCGTCAGCTGAAGCAACTCCG
GCAACTGCCGCTCCTGTAGCAAAACCGCAGGCTGTACCAAACGCGGTATCTATCGCGGAGCTGGTATCCCCGATTACCGG
GGATGTTGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGA
CAGATAAAATCGTCGTATCACCAGCCGCAGGAACAATCGTGAAAATCTTCAACACCAACCACGCATTCTGTCTGGAAACC
GAAAAAGGCGCGGAGATCGTCGTCCATATGGGTATCGACACCGTAGCGCTGGAAGGTAAAGGCTTTAAACGTCTGGTGGA
AGAGGGCGCGCAGGTAAGCGCAGGGCAACCGATTCTGGAAATGGATCTGGATTACCTGAACGCTAACGCTCGCTCGATGA
TTAGCCCGGTGGTTTGCAGCAATATCGACGATTTCAGCGGCCTTATCATTAAAGCTCAGGGCCATGTTGTGGCGGGTCAA
ACACCGCTGTATGAAATCAAAAAGTAA

Upstream 100 bases:

>100_bases
GATCTAACAATCTGATTACAGAACATCGGCAGTACAATTTGCAGCAAAATAAAAATACGGCTTTAAACGAGCCAAATAGG
GTTCTCGTAGGGGGAATAAG

Downstream 100 bases:

>100_bases
TCTTCTTTATGCCTGATGCGACGCTTGAGCGTCGCATCCAACAATGACAAGCGGTGGAGATCTTCTCTGCCGCTTTTTTT
TTCATCAATCATTCCCATAA

Product: PTS system N-acetyl glucosamine specific transporter subunits IIABC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 648; Mature: 648

Protein sequence:

>648_residues
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGY
FVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAG
AIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAV
GGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLET
EKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ
TPLYEIKK

Sequences:

>Translated_648_residues
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGY
FVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAG
AIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAV
GGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLET
EKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ
TPLYEIKK
>Mature_648_residues
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGY
FVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAG
AIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAV
GGTDNLKAIDACITRLRLTVVDSARVNDAMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLET
EKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQ
TPLYEIKK

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=648, Percent_Identity=99.2283950617284, Blast_Score=1294, Evalue=0.0,
Organism=Escherichia coli, GI1787343, Length=485, Percent_Identity=40.8247422680412, Blast_Score=367, Evalue=1e-102,
Organism=Escherichia coli, GI1787908, Length=511, Percent_Identity=34.2465753424658, Blast_Score=258, Evalue=1e-69,
Organism=Escherichia coli, GI1788757, Length=149, Percent_Identity=50.3355704697987, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1790159, Length=174, Percent_Identity=36.2068965517241, Blast_Score=113, Evalue=4e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 68331; Mature: 68331

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00371 PTS_EIIA_TYPE_1_HIS ; PS51093 PTS_EIIA_TYPE_1 ; PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGV
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCCC
PDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFI
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCHHHHHH
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTG
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ISLFVATLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIR
HHHHHHHHHHHHCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAVGGTDNLKAIDACITRLRLTV
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHEE
VDSARVNDAMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ECCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCC
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVS
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCEEECCCCCEEEE
PAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILE
CCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCHHH
MDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQTPLYEIKK
HHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCHHCCC
>Mature Secondary Structure
MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQAGGAIFDNLALIFAIGV
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
ASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGIITGLVGGAAYNRWSDIKL
CCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHCCHHCCCCCCCCC
PDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIHAGGEWIVSAGALGSGIFGFI
HHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCHHHHHH
NRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINRFYAGDGTAGMFMSGFFPIMMFG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHHC
LPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGVTEPLEFLFMFLAPLLYLLHALLTG
CCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ISLFVATLLGIHAGFSFSAGAIDYALMYNLPAASQNVWMLLVMGVVFFAIYFVVFSLVIR
HHHHHHHHHHHHCCCCCCCCHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
MFNLKTPGREDKEDEIVTEEANSNTEEGLNQLATNYIAAVGGTDNLKAIDACITRLRLTV
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHEE
VDSARVNDAMCKRLGASGVVKLNKQTIQVIVGAKAESIGDAMKKVVARGPVAAASAEATP
ECCCCCCHHHHHHCCCCCEEEECCEEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCC
ATAAPVAKPQAVPNAVSIAELVSPITGDVVALDQVPDEAFASKAVGDGVAVKPTDKIVVS
CCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHCCCCEEECCCCCEEEE
PAAGTIVKIFNTNHAFCLETEKGAEIVVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILE
CCCCCEEEEEECCCEEEEECCCCCEEEEEECCCEEEECCCCHHHHHHCCCCCCCCCCHHH
MDLDYLNANARSMISPVVCSNIDDFSGLIIKAQGHVVAGQTPLYEIKK
HHHHHHCCCHHHHHHHHHHCCCCCCCCEEEEECCCEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3284790; 3056518; 8905232; 9278503 [H]