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The map label for this gene is nagD

Identifier: 218688481

GI number: 218688481

Start: 678913

End: 679665

Strand: Reverse

Name: nagD

Synonym: ECED1_0656

Alternate gene names: 218688481

Gene position: 679665-678913 (Counterclockwise)

Preceding gene: 218688482

Following gene: 218688480

Centisome position: 13.05

GC content: 53.52

Gene sequence:

>753_bases
ATGACCATTAAAAATGTAATTTGCGATATCGACGGCGTGCTGATGCACGATAACGTCGCCGTACCGGGTGCAGCGGAATT
TTTGCACGGGATTATGGATAAAGGCCTGCCGCTGGTGTTGCTGACCAACTATCCTTCGCAGACCGGGCAAGATCTGGCGA
ACCGCTTTGCCACCGCTGGTGTCGATGTACCGGATAGCGTGTTTTATACCTCCGCGATGGCGACCGCCGATTTCCTGCGT
CGCCAGGAAGGCAAGAAAGCGTATGTGGTGGGCGAAGGCGCACTGATTCATGAACTGTACAAAGCCGGTTTCACTATTAC
CGATGTGAACCCTGATTTTGTGATTGTTGGCGAAACGCGTTCCTACAACTGGGACATGATGCATAAAGCAGCCTATTTCG
TCGCTAACGGTGCACGCTTTATCGCCACCAACCCGGACACCCACGGGCGCGGTTTTTATCCCGCCTGTGGCGCGTTGTGT
GCAGGGATCGAGAAAATCTCCGGGCGCAAACCGTTCTATGTTGGAAAGCCGAGTCCGTGGATCATCCGCGCGGCATTAAA
CAAAATGCAGGCGCATTCGGAAGAAACGGTGATTGTCGGCGATAACTTGCGTACCGATATTCTGGCGGGCTTCCAGGCCG
GTCTGGAGACGATTCTGGTGCTTTCTGGTGTTTCGTCACTCGACGATATCGACAGTATGCCGTTCCGCCCAAGTTGGATT
TACCCATCGGTCGCTGAAATCGACGTTATCTGA

Upstream 100 bases:

>100_bases
TAAAACGCGCCATGCTCAACGGTATTTTGCTCCAGCATTTGCTGGAAAATTAATGTGCTTTTATAGTGGCGCTTATTGTT
GTCAATATTCTGGGTAGTCC

Downstream 100 bases:

>100_bases
ATAGCAACCACGCCTCAGGGCGTGGTTTCTTATTTTTCCCCATCATTAAAATCACCCCATTCAATAAAACCCGTCAAAAA
TTATCGATCCATCAATAAAT

Product: UMP phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNYPSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLR
RQEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWI
YPSVAEIDVI

Sequences:

>Translated_250_residues
MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNYPSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLR
RQEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWI
YPSVAEIDVI
>Mature_249_residues
TIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNYPSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRR
QEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALCA
GIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWIY
PSVAEIDVI

Specific function: Unknown

COG id: COG0647

COG function: function code G; Predicted sugar phosphatases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI10092677, Length=252, Percent_Identity=29.7619047619048, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI108796653, Length=265, Percent_Identity=31.3207547169811, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI14149777, Length=247, Percent_Identity=25.5060728744939, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786890, Length=250, Percent_Identity=100, Blast_Score=518, Evalue=1e-148,
Organism=Caenorhabditis elegans, GI17560956, Length=263, Percent_Identity=27.3764258555133, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17558880, Length=263, Percent_Identity=27.3764258555133, Blast_Score=90, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17562458, Length=263, Percent_Identity=27.3764258555133, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71984613, Length=264, Percent_Identity=28.030303030303, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562356, Length=233, Percent_Identity=23.6051502145923, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI193210059, Length=258, Percent_Identity=23.6434108527132, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6319965, Length=234, Percent_Identity=27.3504273504274, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24666141, Length=253, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24656326, Length=258, Percent_Identity=26.7441860465116, Blast_Score=97, Evalue=9e-21,
Organism=Drosophila melanogaster, GI22026920, Length=243, Percent_Identity=28.3950617283951, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24666137, Length=291, Percent_Identity=27.4914089347079, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24656330, Length=275, Percent_Identity=27.2727272727273, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19920940, Length=261, Percent_Identity=23.3716475095785, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NAGD_ECO57 (P0AF25)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   A85567
- PIR:   A90717
- RefSeq:   NP_286389.1
- RefSeq:   NP_308732.1
- ProteinModelPortal:   P0AF25
- SMR:   P0AF25
- EnsemblBacteria:   EBESCT00000028253
- EnsemblBacteria:   EBESCT00000059426
- GeneID:   917074
- GeneID:   957741
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0822
- KEGG:   ecs:ECs0705
- GeneTree:   EBGT00050000009288
- HOGENOM:   HBG646794
- OMA:   HKDEAIM
- ProtClustDB:   PRK10444
- BioCyc:   ECOL83334:ECS0705-MONOMER
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006357
- InterPro:   IPR023215
- Gene3D:   G3DSA:3.40.50.1000
- Gene3D:   G3DSA:3.40.50.10410
- TIGRFAMs:   TIGR01460

Pfam domain/function: PF00702 Hydrolase; SSF56784 SSF56784

EC number: NA

Molecular weight: Translated: 27163; Mature: 27032

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNYPSQTGQDLANRFATAG
CCHHHEEECCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCC
VDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETR
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEEECCCCEEEEECCC
SYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALCAGIEKISGRKPFYVGKPSPW
CCCHHHHHHHEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCC
IIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWI
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
YPSVAEIDVI
CCCHHEEECC
>Mature Secondary Structure 
TIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNYPSQTGQDLANRFATAG
CHHHEEECCCCEEEECCCCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHCC
VDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKAGFTITDVNPDFVIVGETR
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCCEEEECCCCEEEEECCC
SYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALCAGIEKISGRKPFYVGKPSPW
CCCHHHHHHHEEEEECCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCC
IIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETILVLSGVSSLDDIDSMPFRPSWI
HHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
YPSVAEIDVI
CCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796