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The map label for this gene is miaB [H]

Identifier: 218688478

GI number: 218688478

Start: 673008

End: 674432

Strand: Reverse

Name: miaB [H]

Synonym: ECED1_0652

Alternate gene names: 218688478

Gene position: 674432-673008 (Counterclockwise)

Preceding gene: 218688480

Following gene: 218688477

Centisome position: 12.95

GC content: 54.67

Gene sequence:

>1425_bases
ATGACCAAAAAACTCCATATTAAAACCTGGGGCTGTCAGATGAACGAGTACGATTCATCGAAGATGGCCGATCTGCTGGA
TGCCACCCACGGCTATCAACTGACCGACGTGGCGGAAGAAGCGGATGTGCTGCTGCTGAACACCTGCTCAATCCGCGAGA
AGGCTCAGGAAAAAGTCTTCCATCAGTTGGGTCGCTGGAAACTGTTAAAAGAGAAGAATCCAGACCTGATTATCGGCGTC
GGCGGCTGCGTGGCATCGCAAGAAGGCGAGCACATTCGCCAGCGCGCCCACTATGTCGATATTATTTTTGGGCCGCAAAC
GCTGCACCGCCTGCCGGAGATGATCAACTCCGTGCGCGGCGACCGCAGCCCGGTTGTAGATATCAGCTTCCCGGAAATCG
AGAAGTTTGACCGTCTGCCAGAACCGCGCGCCGAAGGGCCGACCGCGTTTGTCTCCATCATGGAAGGCTGCAATAAATAT
TGCACCTACTGCGTGGTGCCTTACACCCGTGGTGAAGAGGTAAGCCGTCCGTCCGACGATATTCTGTTTGAGATCGCCCA
GCTTGCGGCTCAGGGCGTGCGTGAAGTCAACCTGCTCGGTCAGAATGTGAATGCCTGGCGTGGTGAGAACTACGACGGCA
CCACCGGAACGTTTGCCGATCTGCTGCGTCTGGTTGCCGCCATCGACGGGATCGATCGTATTCGCTTTACCACCAGCCAT
CCGATCGAATTCACCGACGATATCATCGAAGTGTATCGCGACACGCCGGAGCTGGTGAGCTTCCTGCACCTGCCGGTACA
GAGCGGTTCCGATCGCATTCTGAACCTGATGGGCCGTACTCACACGGCGCTGGAGTACAAAGCGATCATCCGTAAACTGC
GTGCGGCGCGTCCGGATATTCAGATCAGTTCTGACTTCATCGTTGGCTTCCCTGGCGAAACCACCGAAGACTTCGAGAAA
ACGATGAAGCTGATTGCCGACGTCAATTTCGACATGAGCTACAGCTTTATCTTCTCTGCACGTCCGGGTACACCAGCCGC
CGATATGGTTGATGATGTTCCGGAAGAAGAGAAGAAGCAGCGTCTGTATATTCTGCAAGAGCGCATTAATCAGCAAGCGA
TGGCATGGAGCCGCCGGATGCTCGGCACCACCCAGCGCATTCTGGTAGAAGGGACTTCACGTAAGAGCATCATGGAGCTT
TCCGGTCGCACTGAAAATAACCGCGTGGTTAACTTCGAGGGCACGCCGGATATGATCGGTAAATTCGTCGATGTAGAAAT
TACCGACGTCTACCCGAACTCTCTGCGCGGTAAAGTGGTGCGAACTGAAGATGAAATGGGTCTGCGCGTGGCAGAAACAC
CGGAATCGGTGATTGCCCGTACCCGCAAAGAAAACGACCTTGGCGTGGGTTATTATCAGCCGTAA

Upstream 100 bases:

>100_bases
TACCCCCAAGGGCTGATAACGCTCATACTGGTCACAACGGCAGCAAAGCATTACACTATGCGCCCTGCATTCCTGGCTAC
TATTTCGCAAGAGCAAGTCG

Downstream 100 bases:

>100_bases
TTCTCAGGCCCGCCGTTCCGGTGGGCCTTGTTTTTTCTCTCTTTATCCCCATCTTTATCGCAATGCTTGCGCCTTTTTCC
TGTGGCGTGAATAATTTCCT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGV
GGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK
TMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMEL
SGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP

Sequences:

>Translated_474_residues
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGV
GGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSH
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEK
TMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMEL
SGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
>Mature_473_residues
TKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVG
GCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYC
TYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP
IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKT
MKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELS
GRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=481, Percent_Identity=37.4220374220374, Blast_Score=292, Evalue=4e-79,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=37.4476987447699, Blast_Score=291, Evalue=6e-79,
Organism=Homo sapiens, GI93277076, Length=456, Percent_Identity=30.4824561403509, Blast_Score=180, Evalue=2e-45,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=99.789029535865, Blast_Score=983, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=385, Percent_Identity=28.051948051948, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=462, Percent_Identity=33.3333333333333, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI71996771, Length=345, Percent_Identity=27.8260869565217, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=433, Percent_Identity=37.1824480369515, Blast_Score=278, Evalue=6e-75,
Organism=Drosophila melanogaster, GI19922432, Length=493, Percent_Identity=28.6004056795132, Blast_Score=162, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 53678; Mature: 53546

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
CCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCEEEEECCHHHHHHHHHHH
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
HHHCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHH
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHC
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
CEEEEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVF
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCEEHHHHCCCCEEEEECCHHHHHHHHHHH
HQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRG
HHHCCEEEECCCCCCEEEECCCCCCCCCHHHHHHHCCEEEEEECHHHHHHHHHHHHHHCC
DRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDD
CCCCEEECCCCCHHHHHCCCCCCCCCHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCHH
ILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCHHEEEECCC
PIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDI
CCCHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
QISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQ
EECCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCCHHHHH
RLYILQERINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMIG
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHCCCCCCCEEEEECCCHHHHC
KFVDVEITDVYPNSLRGKVVRTEDEMGLRVAETPESVIARTRKENDLGVGYYQP
CEEEEEEEECCCCCCCCCEEEEHHHCCCEEECCCHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA