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The map label for this gene is citT [H]

Identifier: 218688436

GI number: 218688436

Start: 632265

End: 633728

Strand: Reverse

Name: citT [H]

Synonym: ECED1_0609

Alternate gene names: 218688436

Gene position: 633728-632265 (Counterclockwise)

Preceding gene: 218688437

Following gene: 218688435

Centisome position: 12.16

GC content: 51.09

Gene sequence:

>1464_bases
ATGTCTTTAGCAAAAGATAATATATGGAAATTATTGGCGCCCCTGGTGGTGATGGGTGTCATGTTTCTTATCCCTGTACC
TGACGGTATGCCGCCGCAGGCGTGGCATTACTTTGCAGTGTTTGTGGCAATGATTGTCGGCATGATCCTCGAGCCAATTC
CGGCAACGGCGATCAGTTTTATTGCGGTTACTATTTGCGTTATTGGCAGTAATTACCTGCTCTTTGATGCCAAAGAATTA
GCTGACCCAGCGTTTAATGCGCAAAAACAGGCGCTGCAATGGGGTCTGGCTGGTTTTTCCAGCACCACGGTATGGCTGGT
ATTTGGCGCATTTATTTTTGCATTAGGGTATGAAGTTTCCGGGTTAGGTCGTCGCATTGCCCTTTTCCTGGTGAAATTCA
TGGGCAAACGCACGCTGACGTTGGGTTATGCGATTGTCATTATCGACATTCTGCTGGCACCGTTTACACCGTCCAACACC
GCGCGTACCGGGGGTACGGTTTTTCCGGTCATTAAAAACCTGCCGCCGCTGTTTAAATCATTCCCGAACGATCCGTCCGC
GCGTCGTATTGGCGGCTATTTGATGTGGATGATGGTCATTAGTACCAGTCTGAGTTCGTCCATGTTTGTCACCGGTGCGG
CACCAAACGTGCTGGGTCTGGAGTTCGTCAGCAAAATTGCCGGTATCCAGATTAGCTGGTTGCAGTGGTTCCTCTGCTTC
CTGCCGGTTGGGGTTATCTTGCTTATCATTGCGCCGTGGCTTTCCTACGTGCTGTACAAACCGGAAATCACACACAGTGA
AGAAGTGGCAACCTGGGCGGGTGATGAACTAAAAACCATGGGTGCGCTGACGCGCAGAGAGTGGACGCTGATTGGCCTTG
TATTGCTCAGCTTAGGTTTGTGGGTATTTGGCAGTGAAGTCATTAATGCTACTGCGGTAGGTCTGCTGGCAGTTTCGCTG
ATGCTGGCGCTGCACGTTGTGCCGTGGAAAGACATTACTCGCTATAACAGCGCATGGAACACGCTGGTCAACCTGGCAAC
GCTGGTTGTTATGGCGAATGGCCTGACCCGTTCTGGCTTTATCGACTGGTTCGCCAACACGATGAGTACACACCTGGAAG
GCTTCTCACCAAACGCAACGGTGATTGTACTGGTTCTGGTGTTCTACTTTGCACACTACCTGTTTGCCAGCCTGTCTGCG
CACACCGCAACCATGCTGCCGGTTATTCTGGCCGTCGGTAAAGGGATTCCGGGCGTACCAATGGAACAACTGTGTATCCT
GCTGGTACTGTCTATCGGTATCATGGGCTGTCTGACGCCGTATGCAACCGGTCCTGGGGTGATTATTTACGGCTGTGGCT
ATGTGAAATCAAAAGATTACTGGCGTCTTGGCGCAATCTTCGGGGTGATTTACATCTCTATGCTGCTGTTGGTTGGCTGG
CCGATTCTCGCCATGTGGAACTAA

Upstream 100 bases:

>100_bases
GCGGCAGTGCCGACCTGCTGATTCTTACCTGGTTTTTAGCACAGATTTAATTATTTAAGCACTTGATAAATTTGGAAATA
TTAATTTTCGGAGAACCCGT

Downstream 100 bases:

>100_bases
TACGTTCGCCAAACGCTGAATAAATCATCACGCCCGCCAGGTAAGCCCACCAGGCGGGCTTTTTTATGATTTAATAGATA
ATATTCAGTGTATGTATGAG

Product: citrate:succinate antiporter

Products: NA

Alternate protein names: Citrate transporter; Citrate/succinate antiporter [H]

Number of amino acids: Translated: 487; Mature: 486

Protein sequence:

>487_residues
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKEL
ADPAFNAQKQALQWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSA
HTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN

Sequences:

>Translated_487_residues
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKEL
ADPAFNAQKQALQWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSL
MLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSA
HTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
PILAMWN
>Mature_486_residues
SLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELA
DPAFNAQKQALQWGLAGFSSTTVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTA
RTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCFL
PVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLM
LALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGFIDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAH
TATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGWP
ILAMWN

Specific function: Responsible for the uptake of citrate in exchange to the efflux of succinate. Has a relatively broad specificity for C(4)- dicarboxylates and tricarboxylates [H]

COG id: COG0471

COG function: function code P; Di- and tricarboxylate transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SLC13A transporter (TC 2.A.47) family. SODIT1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786829, Length=487, Percent_Identity=99.5893223819302, Blast_Score=964, Evalue=0.0,
Organism=Escherichia coli, GI1789444, Length=471, Percent_Identity=44.7983014861996, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1786986, Length=493, Percent_Identity=33.4685598377282, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001898 [H]

Pfam domain/function: PF00939 Na_sulph_symp [H]

EC number: NA

Molecular weight: Translated: 53150; Mature: 53019

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDAKELADPAFNAQKQALQWGLAGFSSTTVWLVFGAFIFALGYEVS
HHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PILAMWN
CHHCCCC
>Mature Secondary Structure 
SLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAMIVGMILEPIPATAISF
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IAVTICVIGSNYLLFDAKELADPAFNAQKQALQWGLAGFSSTTVWLVFGAFIFALGYEVS
HHHHHHHHCCCEEEEEHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
GLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNTARTGGTVFPVIKNLPPLFKS
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEHHHHHCCCHHHHC
FPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVLGLEFVSKIAGIQISWLQWFLCF
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHH
LPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDELKTMGALTRREWTLIGLVLLSLGL
HHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
WVFGSEVINATAVGLLAVSLMLALHVVPWKDITRYNSAWNTLVNLATLVVMANGLTRSGF
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHH
IDWFANTMSTHLEGFSPNATVIVLVLVFYFAHYLFASLSAHTATMLPVILAVGKGIPGVP
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MEQLCILLVLSIGIMGCLTPYATGPGVIIYGCGYVKSKDYWRLGAIFGVIYISMLLLVGW
HHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
PILAMWN
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]