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The map label for this gene is gcl [H]

Identifier: 218688367

GI number: 218688367

Start: 548808

End: 550676

Strand: Direct

Name: gcl [H]

Synonym: ECED1_0528

Alternate gene names: 218688367

Gene position: 548808-550676 (Clockwise)

Preceding gene: 218688366

Following gene: 218688368

Centisome position: 10.53

GC content: 52.27

Gene sequence:

>1869_bases
GTGTCTGTCGTATCCCGCTCTGCGGAGCGGGTTTTTTTGACAAAATTTGAGAGTTGGAAAATTTTTCCAATTAATAGAGG
TAGGAATAAAATGGCAAAAATGAGAGCCGTTGACGCGGCAATGTATGTGCTGGAGAAAGAAGGTATCACCACCGCCTTCG
GTGTTCCGGGAGCTGCAATCAATCCGTTCTACTCAGCGATGCGTAAGCACGGCGGTATTCGTCACATTCTGGCGCGTCAT
GTGGAAGGTGCTTCGCACATGGCGGAAGGTTATACCCGCGCAACGGCAGGGAATATCGGCGTATGTCTGGGGACTTCCGG
TCCTGCGGGCACGGACATGATCACCGCGCTCTATTCCGCTTCTGCTGATTCCATTCCTATTCTGTGTATTACCGGCCAGG
CTCCGCGCGCCCGTCTGCATAAAGAAGATTTCCAGGCCGTTGATATTGAAGCAATTGCTAAACCGGTCAGCAAAATGGCG
GTTACAGTTCGTGAAGCGGCGCTGGTGCCTCGTGTGCTGCAACAGGCGTTTCACCTGATGCGTTCTGGTCGACCGGGTCC
GGTACTGGTGGATTTACCGTTCGACGTACAGGTTGCGGAAATCGAGTTTGATCCTGATATGTACGAACCGCTGCCGGTCT
ACAAACCTGCTGCCAGCCGTATGCAGATCGAAAAAGCTGTAGAAATGTTAATCCAGGCCGAACGTCCGGTGATTGTTGCC
GGAGGCGGGGTAATTAATGCTGACGCAGCTGTACTGTTACAACAGTTTGCTGAACTGACCAGCATTCCGGTGATCCCAAC
GCTGATGGGCTGGGGCTGTATCCCGGACGATCATGAACTGATGGCCGGGATGGTGGGCCTGCAAACCGCGCATCGTTACG
GTAATGCAACGTTGCTGGCGTCCGACATGGTGTTTGGTATCGGTAACCGTTTTGCTAATCGTCATACCGGTTCGGTAGAG
AAATACACCGAAGGGCGCAAAATCGTTCATATCGATATTGAGCCGACGCAAATTGGCCGCGTACTGTGTCCGGATCTTGG
CATTGTCTCTGATGCTAAAGCGGCGCTGACGCTGCTGGTTGAAGTGGCGCAGGAGATGCAAAAAGCCGGTCGTCTGCCGT
GTCGTAAAGAGTGGGTCGCCGACTGCCAGCAGCGCAAACGCACTTTGCTGCGCAAAACCCACTTCGACAACGTGCCGGTG
AAACCGCAGCGCGTGTATGAAGAGATGAACAAAGCCTTTGGCCGCGATGTTTGTTATGTCACCACCATTGGCCTGTCACA
AATCGCTGCGGCACAAATGCTGCATGTCTTTAAAGACCGCCACTGGATCAACTGTGGGCAGGCAGGCCCGCTGGGCTGGA
CGATTCCGGCGGCGCTGGGGGTTTGTGCCGCTGATCCAGAGCGCAATGTGGTGGCGATTTCCGGTGACTTCGACTTCCAG
TTCCTGATTGAAGAATTAGCCGTTGGCGCACAGTTCAACATTCCGTACATCCATGTACTGGTCAATAACGCCTATCTGGG
TCTGATTCGCCAGTTGCAACGTGCCTTTGATATGGATTACTGCGTACAGCTCGCGTTCGAAAATATTAACTCCAGCGAAG
TGAACGGTTATGGCGTCGACCACGTAAAAGTAGCGGAAGGTTTAGGTTGTAAAGCAATTCGCGTTTTCAAACCGGAAGAC
ATTGCCAAAATTGGTCACCTGCAAATTGCTGATAATCCGCATCGCGGCGAACCGGGAACCGGAGAAATTAATTACGAATA
TCTCTTTACGGTAATTGAAAATTCTGACTACAACGGTTGGGTTGGCTGTGAATATAAACCCCAAACCACCACGGAAGCCG
GTTTACGCTGGATGGATCCGTACCGTTAA

Upstream 100 bases:

>100_bases
CATCAAGACTGACAGAAGATCGTTTTGTCAGCCAGGGTGAGCTGGTCAGAGACACCGCCCGCGATATCAGCACGGCGTTG
GGACTGAAAGCACATCCATA

Downstream 100 bases:

>100_bases
AACGTAACGCTATTCAGACAATGCTTTTTTAGGCCGCTAAGTTGGCAGGGGATCGTGTTGTCTGAATTCAGGAAAAGCGA
AATTTAAAAGAGGTTAATTA

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MSVVSRSAERVFLTKFESWKIFPINRGRNKMAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARH
VEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMA
VTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVA
GGGVINADAAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVE
KYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPV
KPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQLQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPED
IAKIGHLQIADNPHRGEPGTGEINYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR

Sequences:

>Translated_622_residues
MSVVSRSAERVFLTKFESWKIFPINRGRNKMAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARH
VEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMA
VTVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVA
GGGVINADAAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVE
KYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPV
KPQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQLQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPED
IAKIGHLQIADNPHRGEPGTGEINYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR
>Mature_621_residues
SVVSRSAERVFLTKFESWKIFPINRGRNKMAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIRHILARHV
EGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAV
TVREAALVPRVLQQAFHLMRSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAG
GGVINADAAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFGIGNRFANRHTGSVEK
YTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVK
PQRVYEEMNKAFGRDVCYVTTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQF
LIEELAVGAQFNIPYIHVLVNNAYLGLIRQLQRAFDMDYCVQLAFENINSSEVNGYGVDHVKVAEGLGCKAIRVFKPEDI
AKIGHLQIADNPHRGEPGTGEINYEYLFTVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYR

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=492, Percent_Identity=23.3739837398374, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI21361361, Length=570, Percent_Identity=23.859649122807, Blast_Score=107, Evalue=4e-23,
Organism=Homo sapiens, GI300068962, Length=82, Percent_Identity=42.6829268292683, Blast_Score=73, Evalue=7e-13,
Organism=Escherichia coli, GI1786717, Length=532, Percent_Identity=99.2481203007519, Blast_Score=1092, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=543, Percent_Identity=34.4383057090239, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1790104, Length=482, Percent_Identity=35.6846473029046, Blast_Score=281, Evalue=6e-77,
Organism=Escherichia coli, GI1787096, Length=522, Percent_Identity=25.4789272030651, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786718, Length=145, Percent_Identity=52.4137931034483, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788716, Length=473, Percent_Identity=26.215644820296, Blast_Score=134, Evalue=2e-32,
Organism=Escherichia coli, GI1789095, Length=82, Percent_Identity=43.9024390243902, Blast_Score=76, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=506, Percent_Identity=24.1106719367589, Blast_Score=118, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI17531301, Length=506, Percent_Identity=24.1106719367589, Blast_Score=118, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=543, Percent_Identity=24.6777163904236, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=547, Percent_Identity=32.7239488117002, Blast_Score=260, Evalue=6e-70,
Organism=Saccharomyces cerevisiae, GI6320816, Length=480, Percent_Identity=24.375, Blast_Score=97, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6323163, Length=492, Percent_Identity=22.9674796747967, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6321524, Length=484, Percent_Identity=21.900826446281, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI19922626, Length=487, Percent_Identity=24.2299794661191, Blast_Score=135, Evalue=7e-32,
Organism=Drosophila melanogaster, GI17530883, Length=79, Percent_Identity=44.3037974683544, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 68450; Mature: 68319

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVSRSAERVFLTKFESWKIFPINRGRNKMAKMRAVDAAMYVLEKEGITTAFGVPGAAI
CCCCCCCCCEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHH
NPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSA
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCEECCCCEEEEEECCCCCCHHHHHHHHHC
SADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLM
CCCCCEEEEEECCCCHHHHCHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVA
HCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
GGGVINADAAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLA
CCCEECCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
SDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLV
HHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCHHHHCHHHCCCCCCCCCHHHHHHHHH
EVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEE
TTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
EECCHHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHHHHHHHCCCCCCCEEEEECCCCHH
FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQLQRAFDMDYCVQLAFENINSSEVNGYGVD
HHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
HVKVAEGLGCKAIRVFKPEDIAKIGHLQIADNPHRGEPGTGEINYEYLFTVIENSDYNGW
EEECCCCCCCCEEEEECCHHHHHHCCEEECCCCCCCCCCCCEEEEEEEEEEEECCCCCCE
VGCEYKPQTTTEAGLRWMDPYR
ECCEECCCCCHHHCCEECCCCC
>Mature Secondary Structure 
SVVSRSAERVFLTKFESWKIFPINRGRNKMAKMRAVDAAMYVLEKEGITTAFGVPGAAI
CCCCCCCCEEEEEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEECCCCCCHH
NPFYSAMRKHGGIRHILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSA
HHHHHHHHHCCCHHHHHHHHHCCHHHHHHCCCEECCCCEEEEEECCCCCCHHHHHHHHHC
SADSIPILCITGQAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLM
CCCCCEEEEEECCCCHHHHCHHCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSGRPGPVLVDLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVA
HCCCCCCEEEECCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
GGGVINADAAVLLQQFAELTSIPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLA
CCCEECCHHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEE
SDMVFGIGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLV
HHHHHHHHHHHCCCCCCCCHHHCCCCEEEEEECCHHHHCHHHCCCCCCCCCHHHHHHHHH
EVAQEMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYV
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEE
TTIGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERNVVAISGDFDFQ
EECCHHHHHHHHHHHHHHCCCEECCCCCCCCCEEHHHHHHHHCCCCCCCEEEEECCCCHH
FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQLQRAFDMDYCVQLAFENINSSEVNGYGVD
HHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCC
HVKVAEGLGCKAIRVFKPEDIAKIGHLQIADNPHRGEPGTGEINYEYLFTVIENSDYNGW
EEECCCCCCCCEEEEECCHHHHHHCCEEECCCCCCCCCCCCEEEEEEEEEEEECCCCCCE
VGCEYKPQTTTEAGLRWMDPYR
ECCEECCCCCHHHCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]