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The map label for this gene is aes [H]

Identifier: 218688339

GI number: 218688339

Start: 519644

End: 520603

Strand: Reverse

Name: aes [H]

Synonym: ECED1_0499

Alternate gene names: 218688339

Gene position: 520603-519644 (Counterclockwise)

Preceding gene: 218688341

Following gene: 218688330

Centisome position: 9.99

GC content: 51.77

Gene sequence:

>960_bases
ATGAAGCCGGAAAACAAATTACCCGTTCTGGACCTTATCTCTGCTGAAATGAAGACCGTTGTGAATACTCTTCAGCCGGA
TTTACCGCCCTGGCCTGCAACGGGAACGATTGCTGAGCAACGACAGTATTACACGCTTGAGCGCCGATTCTGGAATGTGA
GCGCTCCAGAAATGGCAACCAGAGCTTACAGGGTTCCAACAAAATATGGGCAGGTGAAAACACGTATCTTTTATCCGCAG
CCAGATAGCCCGGCGACGCTATTTTATTTGCATGGAGGCGGTTTTATTCTCGGCAATCTCGATACCCACGATCGCATCAT
GCGCCTGCTGGCAAGCTACAGCCAATGTACGGTGATTGGCATTGATTACACCCTTTCACCTGAAGCGCGTTTTCCGCAAG
CGATAGAGGAAATTGTGGCTGCCTGTTGTTATTTCCACCAGCAGGCGGAGGATTATCAAATCAATATGTCCCGCATTGGT
TTTGCCGGTGATTCCGCAGGTGCCATGCTGGCGCTCGCCAGTGCGTTGTGGTTGCGCGATAAACAGATCGATTGCGGTAA
AGTTGCGGGCGTTTTGCTGTGGTATGGGCTTTACGGATTACGGGATTCCGTGACTCGTCGTCTGTTGGGTGGTGTCTGGG
ATGGCTTAACGCAACAGGATTTGCAGATGTATGAAGAGGCGTATTTAAGCAACGACGCGGACCGCGAGTCGCCATATTAC
TGCCTGTTTAATAATGATCTCACTCGCGAAGTTCCGCCCTGTTTTATTGCCGGGGCGGAGTTCGATCCACTGCTGGATGA
CAGCCGTCTGCTTTACCAGACGTTAGCGGCGCATCAGCAGCCCTGTGAGTTCAAACTCTACCCAGGCACGCTGCACGCCT
TTTTGCATTATTCACGGATGATGAAAACCGCCGACGAGGCTCTTCGCGACGGCGCTCAGTTCTTTACCGCTCAGCTTTAG

Upstream 100 bases:

>100_bases
GGGAGCGCGCGAGACGCAGCCAGTTTCAATATCAGCAGGCGGGATCGCATTTTCATGGCAGTGGTTCCTTACAATGACGT
AATTTGAAAGGAGTTTTTGC

Downstream 100 bases:

>100_bases
CGATACGCGGCAACAAGATTAGCCATCATTTCGATATGTTCCGGCGTGGCATTAAGCGCCGGAATATATTCGTATTTTTT
CCCGCCAGCGCCGAGGAAGA

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNVSAPEMATRAYRVPTKYGQVKTRIFYPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL

Sequences:

>Translated_319_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNVSAPEMATRAYRVPTKYGQVKTRIFYPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL
>Mature_319_residues
MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNVSAPEMATRAYRVPTKYGQVKTRIFYPQ
PDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
FAGDSAGAMLALASALWLRDKQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI68299767, Length=121, Percent_Identity=34.7107438016529, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=98.1191222570533, Blast_Score=653, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540028, Length=142, Percent_Identity=33.0985915492958, Blast_Score=75, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71996133, Length=232, Percent_Identity=26.7241379310345, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI72001146, Length=130, Percent_Identity=31.5384615384615, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24656084, Length=98, Percent_Identity=39.7959183673469, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24656076, Length=98, Percent_Identity=39.7959183673469, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI20130169, Length=98, Percent_Identity=39.7959183673469, Blast_Score=81, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36177; Mature: 36177

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01173 LIPASE_GDXG_HIS ; PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNVSAPEMAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
RAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
HHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
IDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
MKTADEALRDGAQFFTAQL
HHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure
MKPENKLPVLDLISAEMKTVVNTLQPDLPPWPATGTIAEQRQYYTLERRFWNVSAPEMAT
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHH
RAYRVPTKYGQVKTRIFYPQPDSPATLFYLHGGGFILGNLDTHDRIMRLLASYSQCTVIG
HHHCCCCCCCCEEEEEECCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHCCCCEEEEE
IDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIGFAGDSAGAMLALASALWLRD
EEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCHHHHHHHHHHHHCC
KQIDCGKVAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQQDLQMYEEAYLSNDADRESPYY
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLAAHQQPCEFKLYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHH
MKTADEALRDGAQFFTAQL
HHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA