Click here to switch to the map view.

The map label for this gene is mhpB [H]

Identifier: 218688219

GI number: 218688219

Start: 397406

End: 398350

Strand: Direct

Name: mhpB [H]

Synonym: ECED1_0376

Alternate gene names: 218688219

Gene position: 397406-398350 (Clockwise)

Preceding gene: 218688218

Following gene: 218688220

Centisome position: 7.63

GC content: 57.46

Gene sequence:

>945_bases
ATGCACGCTTATCTTCACTGTCTTTCCCACTCGCCGCTGGTGGGGTATGTCGACCCGGCACAAGAGGTACTCGATGAGGT
CAATGGCGTGATTGCCAGCGCCCGCGAGCGTATTGCGGCTTTCTCCCCTGAACTGGTGGTGCTGTTTGCGCCAGATCACT
ACAACGGCTTTTTCTATGACGTGATGCCACCGTTCTGTTTAGGCGTTGGAGCGACGGCAATTGGTGATTTCGGCAGTGCG
GCAGGTGAGCTGCCCGTGCCTGTGGAGCTGGCGGAGGCCTGTGCGCATGCCGTCATGAAGAGCGGAATCGATCTTGCCGT
TTCTTACTGTATGCAGGTGGACCACGGGTTCGCCCAGCCGCTGGAGTTCCTGCTCGGTGGGCTGGATAAGGTGCCAGTTC
TGCCTGTGTTCATCAACGGTGTCGCCACGCCGCTGCCCGGTTTCCAGCGTACCCGCATGTTGGGTGAAGCCATTGGACGT
TTCACCAGCACTCTCAATAAACGCGTGCTGTTCCTTGGGGCCGGTGGGCTTTCCCATCAGCCGCCGGTGCCAGAACTGGC
GAAAGCCGATGCCCATATGCGCGACCGTCTGTTGGGGAGCGGGAAAGATTTACCCGCCACTGAGCGTGAATTGCGTCAGC
AGCGGGTGATTAGCGCCGCTGAGAAGTTTGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAG
TTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAA
GTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTT
ATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGA

Upstream 100 bases:

>100_bases
GCCATTCCGCAAACCCTGGGCAAGACCCTGAATAAACTGGCGTCGGTGATGACGCTGACCCGCCCTGATGCCGACGTTTC
TGTCGAAAAGGTAGCCTGAT

Downstream 100 bases:

>100_bases
ATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGT
AAAACGCTGCGCATCCATTT

Product: 3-(2,3-dihydroxyphenyl)propionate dioxygenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSA
AGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
FTSTLNKRVLFLGAGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN

Sequences:

>Translated_314_residues
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSA
AGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
FTSTLNKRVLFLGAGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN
>Mature_314_residues
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSA
AGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
FTSTLNKRVLFLGAGGLSHQPPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN

Specific function: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3- dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2- hydroxy-6-ketononatrienedioate, respectively [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ligB/mhpB extradiol dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786544, Length=314, Percent_Identity=99.3630573248408, Blast_Score=643, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004183 [H]

Pfam domain/function: PF02900 LigB [H]

EC number: =1.13.11.16 [H]

Molecular weight: Translated: 34194; Mature: 34194

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYD
CCHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
VMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQP
CCCHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHH
LEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGRFTSTLNKRVLFLGAGGLSHQ
HHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPE
HHHHHHHCCHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHCCCEECCCHH
WIAGFGSLSARTEN
HHHHHCCCCCCCCC
>Mature Secondary Structure
MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPELVVLFAPDHYNGFFYD
CCHHHHHHCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEE
VMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSGIDLAVSYCMQVDHGFAQP
CCCHHHHHCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHH
LEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGRFTSTLNKRVLFLGAGGLSHQ
HHHHHCCCCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
PPVPELAKADAHMRDRLLGSGKDLPATERELRQQRVISAAEKFVEDQRTLHPLNPIWDNQ
CCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWVAAFAAISAFGNWRSEGRYYRPIPE
HHHHHHHCCHHHHHHCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCHHHCCCEECCCHH
WIAGFGSLSARTEN
HHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA