Click here to switch to the map view.

The map label for this gene is yahI [H]

Identifier: 218688195

GI number: 218688195

Start: 367090

End: 368040

Strand: Direct

Name: yahI [H]

Synonym: ECED1_0352

Alternate gene names: 218688195

Gene position: 367090-368040 (Clockwise)

Preceding gene: 218688194

Following gene: 218688196

Centisome position: 7.05

GC content: 56.68

Gene sequence:

>951_bases
ATGAAAGAACTTGTGGTCGTTGCCATTGGTGGCAACAGCATTATCAAAGATAACGCCAGCCAGTCGATTGAGCATCAGGC
GGAGGCGGTGAAAGCCGTGGCCGATACGGTGCTGGAAATGCTGGCTTCCGATTACGACATTGTGCTGACCCACGGCAACG
GGCCGCAGGTCGGGCTGGATTTACGCCGCGCGGAGATTGCCCACGAGCGCGAAGGGCTGCCCTTAACGCCGCTGGCGAAC
TGTGTGGCGGATACGCAAGGTGGCATTGGCTATCTGATCCAGCAGGCGCTGAACAACCGACTGGCGCGTCACGGCGAGAA
AAAAGCCGTCACCGTGGTGACTCAGGTGGAAGTGGATAAAAACGATCCGGGGTTTGCCCATCCCACCAAGCCCATCGGCG
AATTCTTTAGTGAAAGCCAGCGTGACGAACTACAAAAGGCAAACCCTGACTGGCGTTTTGTTGAAGATGCCGGACGGGGC
TATCGCCGCGTGGTCGCCTCGCCGGAACCGAAACGTATTGTCGAAGCACCTGCCATTAAGGCGCTGATCCAACAAGGCTT
TGTGGTGATTGGCGCGGGCGGCGGCGGAATTCCGGTAGTGCGTACTGACGCGGGGGATTACCAAAGCGTGGACGCGGTTA
TCGACAAAGATTTCTCTACCGCGCTACTGGCTCGCGAAATTCACGCCGACATTCTGGTGATCACCACTGGCGTCGAAAAA
GTGTGTATTCACTTTGGCAAACCGCAGCAGCAGGCGCTGGATCGCGTAGATATTGCCACCATGACCCGCTATATGCAGGA
AGGGCATTTCCCACCTGGCAGCATGTTGCCAAAAATCATCGCCAGCCTGACGTTCCTGGAACAAGGCGGCAAAGAAGTGA
TTATCACCACGCCGGAATGCCTGCCTGCGGCGCTACGCGGCGAAACGGGCACCCATATTATTAAAACCTAA

Upstream 100 bases:

>100_bases
CTACGAGTGGCGCAAGAATCTGTAGGCCTGATAAGCGTAGCGCATCAGGCAGTCTGGCGTTGGTCATAACCCCATCACCC
TCTGTCGCGGACATAACAAC

Downstream 100 bases:

>100_bases
GGACGTAAGATGAAAGAAAACAATAGCCGCCGTGAATTTCTGAGCCAGAGCGGTAAGATGGTTACCGCCGCCGCGCTGTT
TGGTCCCTCAGTGCCGCTCG

Product: putative carbamate kinase

Products: ADP; carbamoyl phosphate

Alternate protein names: NA

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDFSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT

Sequences:

>Translated_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDFSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT
>Mature_316_residues
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLAN
CVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRG
YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDFSTALLAREIHADILVITTGVEK
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT

Specific function: Unknown

COG id: COG0549

COG function: function code E; Carbamate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbamate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786516, Length=316, Percent_Identity=98.4177215189873, Blast_Score=634, Evalue=0.0,
Organism=Escherichia coli, GI1789238, Length=318, Percent_Identity=45.5974842767296, Blast_Score=259, Evalue=1e-70,
Organism=Escherichia coli, GI1786732, Length=313, Percent_Identity=38.0191693290735, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR003964 [H]

Pfam domain/function: PF00696 AA_kinase [H]

EC number: 2.7.2.2

Molecular weight: Translated: 34091; Mature: 34091

Theoretical pI: Translated: 5.61; Mature: 5.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHHH
ALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDFSTALLAREIHADILVITTGVEK
HHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEECCHHH
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGETGTHIIKT
HHHHHCCCCCCEEECC
>Mature Secondary Structure
MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASDYDIVLTHGNGPQVGLD
CCCEEEEEECCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHH
LRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLARHGEKKAVTVVTQVEVDK
HHHHHHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECC
NDPGFAHPTKPIGEFFSESQRDELQKANPDWRFVEDAGRGYRRVVASPEPKRIVEAPAIK
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHCCCCEEECCCCCCHHHHHHHHHH
ALIQQGFVVIGAGGGGIPVVRTDAGDYQSVDAVIDKDFSTALLAREIHADILVITTGVEK
HHHHCCCEEEEECCCCCCEEEECCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEECCHHH
VCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSMLPKIIASLTFLEQGGKEVIITTPEC
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHH
LPAALRGETGTHIIKT
HHHHHCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; NH3; CO2

Specific reaction: ATP + NH3 + CO2 = ADP + carbamoyl phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]