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The map label for this gene is iolD [H]

Identifier: 218688160

GI number: 218688160

Start: 330931

End: 332880

Strand: Direct

Name: iolD [H]

Synonym: ECED1_0314

Alternate gene names: 218688160

Gene position: 330931-332880 (Clockwise)

Preceding gene: 218688156

Following gene: 218688161

Centisome position: 6.35

GC content: 56.1

Gene sequence:

>1950_bases
GTGGGGTACATGAAAACAATCAGGTTGACCATGGCGCAGGCTTTGGTGCGCTTTCTTGATAATCAGTACATCGACGTAGA
CGGCAGCGAAATCAAATTTGTAAAAGGGATTTTCGCCATTTTTGGCCACGGGAACGTCGTTGGATTGGGGCAAGCGCTGG
AAGAGGACTGTGGCCAACTTAGCGTTCATCAAGGGCGTAACGAACAGGGAATGGCGCATATCGCGACGGGATTTGCCCGC
CAGATGCGTCGCCATCAGATTTATGCCTGCACCTCCTCAGTGGGGCCAGGGGCCGCCAATATGATCACCGCAGCGGCGAC
CGCGACGGCTAACCGTATTCCACTGCTTTTGCTGCCGGGAGATGTGTACGCATCTCGTCAACCCGACCCGGTTTTGCAAC
AGGTTGAACAAGAACACGATCTGACGCTGAGCACCAATGACGCTTTCCGTGCAGTTAGCCGCTACTGGGATCGCATTACG
CGTCCGGAACAGCTAATGAGCGCCTGTATCAGCGCGATGCGGGTGTTAACCGATCCGGCGGATACGGGGGCCGTGACGCT
TTGCCTGCCACAGGATGTGCAGGGTGAAGCCTGGGATTATCCGGATTATTTTTTCGCTCGCCGGGTCTATCGTCTTGAGC
GTCACGCGCCGACGGAGCCGATGCTGAACGAGGCGGTTGCGCTGATTCGCCGCCACCAGCGGCCGCTGATCGTTTGCGGT
GGGGGCGTGAAGTATTCGCAGGCTGAAGAGGCGCTGCTGAGATTTGCCGAACGCTGTCATCTGCCGATTGCTGAAACCCA
GGCTGGCAAGGGAGCGCTCAGTTCTGCACACCCGTTGAATGTCGGCGGGATTGGCGAAACCGGTTCACTGGCGGCGAATC
TGCTGGCGCAGGAGGCCGATCTGATTATCGGTGTGGGGACGCGCTATACCGATTTCACCACCTCCTCAAAGTGGATCTTC
CAGAATCCCGACGTGCGCTACTTAAATATCAACGTTAGCCGCTTTGATGTCTTCAAGCTGGATGGCGTACAGATGCAGGG
TGACGCTCGCGTCGCCCTGACGCAGCTTAGCGAACGGCTGGCCCAGGAGCATTATGCTTCGCAATGGGGTGAGACTATTC
ACCGCGTCCGCTCGCAATATATGGCGGAAGTTGAGCGCGTCTATGCTGTGGAATATAGCGGAGAGGGCTTCAAACCTGAA
ATTGAGGATCATATGGATACTCAAAAGGTGTTTGAAGAGTTTAATGAGATTACGCGATCGTGGCTGACCCAGACGCGCGT
GTTGGGCGTGCTTAACCGGATGTTGCCGGAAAACGCGCTGGTGGTGGCGGCGGCGGGCAGCCTGCCGGGCGACCTCCAGC
GTGTCTGGCAAAGCCGCGGAGAGAATGATTACCACGTCGAGTACGGCTACTCCTGTATGGGCTACGAAGTCAATGCCGCA
TTGGGGGCCAAACTGGCGCAGCCGGAGCGCGAGGTGTACAGCTTCGTGGGCGACGGTTCGTTCATGATGCTGCACTCTGA
GTTGGTCACTTCCGTCCAGATGGGGAAAAAGATTACCGTCATTTTGCTCGATAACATGACCAACGGCTGTATCAATAATC
TGCAAATGGAACACGGTATGGACAGTTACTTCACCGAGTTTCGTTTCCATCAGCAGGAGAGCGGTCGTCAGGAAGGCGGG
TTTATCCCGGTCGATTTCGCTCGCATCGCTGAAGGGTATGGCTGTAAAAGCTATCGCGTCACCACCATTGAACAACTGCA
TGAAGCGTTGGAAGATGCTCGTAAACAGGCCGTGAGCACGCTGATAGACATAAAAGTGCTTCCCAAAACGATGGTGCATA
AGTACCTGAGCTGGTGGCGCGTTGGCGGGGCGCAGGTATCCCGTAGCGAACGTATCCAGGCGGTAGCGCGTATGCTTGAG
GAACATATCGGACAGGCCCGGCAGTACTGA

Upstream 100 bases:

>100_bases
TGTGAGAACGGAAATTTTGATCCTATGATAAGTCTCTGTGTTTTTGGGGCTGAAGCGATGCATAAGCAGGCAACGACAGC
CGAACGGTTGATAAAAGCAG

Downstream 100 bases:

>100_bases
TTAATTTGCCGTCGTTCAGGTTGCAGAAGTGTGGTTATTGTTCTGCAACCTGAACGATACAGCGTCAACGCATTGTTTTC
TTTTCAGAACGGCAAAATAA

Product: putative malonic semialdehyde oxidative decarboxylase (iolD-like)

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 649; Mature: 648

Protein sequence:

>649_residues
MGYMKTIRLTMAQALVRFLDNQYIDVDGSEIKFVKGIFAIFGHGNVVGLGQALEEDCGQLSVHQGRNEQGMAHIATGFAR
QMRRHQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDPVLQQVEQEHDLTLSTNDAFRAVSRYWDRIT
RPEQLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHAPTEPMLNEAVALIRRHQRPLIVCG
GGVKYSQAEEALLRFAERCHLPIAETQAGKGALSSAHPLNVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIF
QNPDVRYLNINVSRFDVFKLDGVQMQGDARVALTQLSERLAQEHYASQWGETIHRVRSQYMAEVERVYAVEYSGEGFKPE
IEDHMDTQKVFEEFNEITRSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQRVWQSRGENDYHVEYGYSCMGYEVNAA
LGAKLAQPEREVYSFVGDGSFMMLHSELVTSVQMGKKITVILLDNMTNGCINNLQMEHGMDSYFTEFRFHQQESGRQEGG
FIPVDFARIAEGYGCKSYRVTTIEQLHEALEDARKQAVSTLIDIKVLPKTMVHKYLSWWRVGGAQVSRSERIQAVARMLE
EHIGQARQY

Sequences:

>Translated_649_residues
MGYMKTIRLTMAQALVRFLDNQYIDVDGSEIKFVKGIFAIFGHGNVVGLGQALEEDCGQLSVHQGRNEQGMAHIATGFAR
QMRRHQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDPVLQQVEQEHDLTLSTNDAFRAVSRYWDRIT
RPEQLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHAPTEPMLNEAVALIRRHQRPLIVCG
GGVKYSQAEEALLRFAERCHLPIAETQAGKGALSSAHPLNVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIF
QNPDVRYLNINVSRFDVFKLDGVQMQGDARVALTQLSERLAQEHYASQWGETIHRVRSQYMAEVERVYAVEYSGEGFKPE
IEDHMDTQKVFEEFNEITRSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQRVWQSRGENDYHVEYGYSCMGYEVNAA
LGAKLAQPEREVYSFVGDGSFMMLHSELVTSVQMGKKITVILLDNMTNGCINNLQMEHGMDSYFTEFRFHQQESGRQEGG
FIPVDFARIAEGYGCKSYRVTTIEQLHEALEDARKQAVSTLIDIKVLPKTMVHKYLSWWRVGGAQVSRSERIQAVARMLE
EHIGQARQY
>Mature_648_residues
GYMKTIRLTMAQALVRFLDNQYIDVDGSEIKFVKGIFAIFGHGNVVGLGQALEEDCGQLSVHQGRNEQGMAHIATGFARQ
MRRHQIYACTSSVGPGAANMITAAATATANRIPLLLLPGDVYASRQPDPVLQQVEQEHDLTLSTNDAFRAVSRYWDRITR
PEQLMSACISAMRVLTDPADTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHAPTEPMLNEAVALIRRHQRPLIVCGG
GVKYSQAEEALLRFAERCHLPIAETQAGKGALSSAHPLNVGGIGETGSLAANLLAQEADLIIGVGTRYTDFTTSSKWIFQ
NPDVRYLNINVSRFDVFKLDGVQMQGDARVALTQLSERLAQEHYASQWGETIHRVRSQYMAEVERVYAVEYSGEGFKPEI
EDHMDTQKVFEEFNEITRSWLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQRVWQSRGENDYHVEYGYSCMGYEVNAAL
GAKLAQPEREVYSFVGDGSFMMLHSELVTSVQMGKKITVILLDNMTNGCINNLQMEHGMDSYFTEFRFHQQESGRQEGGF
IPVDFARIAEGYGCKSYRVTTIEQLHEALEDARKQAVSTLIDIKVLPKTMVHKYLSWWRVGGAQVSRSERIQAVARMLEE
HIGQARQY

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=566, Percent_Identity=20.8480565371025, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI21361361, Length=614, Percent_Identity=24.4299674267101, Blast_Score=83, Evalue=8e-16,
Organism=Escherichia coli, GI1790104, Length=601, Percent_Identity=24.459234608985, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI87081685, Length=616, Percent_Identity=26.7857142857143, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1786717, Length=538, Percent_Identity=25.8364312267658, Blast_Score=139, Evalue=6e-34,
Organism=Escherichia coli, GI1787096, Length=533, Percent_Identity=21.7636022514071, Blast_Score=102, Evalue=9e-23,
Organism=Escherichia coli, GI1788716, Length=535, Percent_Identity=21.6822429906542, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17542570, Length=480, Percent_Identity=22.7083333333333, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17531299, Length=449, Percent_Identity=21.6035634743875, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=449, Percent_Identity=21.6035634743875, Blast_Score=69, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6323755, Length=618, Percent_Identity=24.1100323624595, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI19922626, Length=571, Percent_Identity=22.5919439579685, Blast_Score=86, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 72684; Mature: 72553

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYMKTIRLTMAQALVRFLDNQYIDVDGSEIKFVKGIFAIFGHGNVVGLGQALEEDCGQL
CCCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCEEECHHHHHHHHCCE
SVHQGRNEQGMAHIATGFARQMRRHQIYACTSSVGPGAANMITAAATATANRIPLLLLPG
EHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECC
DVYASRQPDPVLQQVEQEHDLTLSTNDAFRAVSRYWDRITRPEQLMSACISAMRVLTDPA
CCCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHAPTEPMLNEAVALIRRHQRPLIVCG
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEC
GGVKYSQAEEALLRFAERCHLPIAETQAGKGALSSAHPLNVGGIGETGSLAANLLAQEAD
CCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
LIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDVFKLDGVQMQGDARVALTQLSERL
EEEECCCCEECCCCCCCEEEECCCEEEEEEEECEEEEEEECCEEECCCHHHHHHHHHHHH
AQEHYASQWGETIHRVRSQYMAEVERVYAVEYSGEGFKPEIEDHMDTQKVFEEFNEITRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHH
WLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQRVWQSRGENDYHVEYGYSCMGYEVNAA
HHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCEEECEEECHH
LGAKLAQPEREVYSFVGDGSFMMLHSELVTSVQMGKKITVILLDNMTNGCINNLQMEHGM
HCCHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEEEEEECCCCCHHHHHCCHHCCC
DSYFTEFRFHQQESGRQEGGFIPVDFARIAEGYGCKSYRVTTIEQLHEALEDARKQAVST
HHHHHHHHHHHHHCCCCCCCEECEEHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
LIDIKVLPKTMVHKYLSWWRVGGAQVSRSERIQAVARMLEEHIGQARQY
HHHHHHCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
GYMKTIRLTMAQALVRFLDNQYIDVDGSEIKFVKGIFAIFGHGNVVGLGQALEEDCGQL
CCHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHCCCCEEECHHHHHHHHCCE
SVHQGRNEQGMAHIATGFARQMRRHQIYACTSSVGPGAANMITAAATATANRIPLLLLPG
EHHCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCEEEEECC
DVYASRQPDPVLQQVEQEHDLTLSTNDAFRAVSRYWDRITRPEQLMSACISAMRVLTDPA
CCCCCCCCCHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DTGAVTLCLPQDVQGEAWDYPDYFFARRVYRLERHAPTEPMLNEAVALIRRHQRPLIVCG
CCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEC
GGVKYSQAEEALLRFAERCHLPIAETQAGKGALSSAHPLNVGGIGETGSLAANLLAQEAD
CCCCCHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCC
LIIGVGTRYTDFTTSSKWIFQNPDVRYLNINVSRFDVFKLDGVQMQGDARVALTQLSERL
EEEECCCCEECCCCCCCEEEECCCEEEEEEEECEEEEEEECCEEECCCHHHHHHHHHHHH
AQEHYASQWGETIHRVRSQYMAEVERVYAVEYSGEGFKPEIEDHMDTQKVFEEFNEITRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHH
WLTQTRVLGVLNRMLPENALVVAAAGSLPGDLQRVWQSRGENDYHVEYGYSCMGYEVNAA
HHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHCCCCCEEEEECCEEECEEECHH
LGAKLAQPEREVYSFVGDGSFMMLHSELVTSVQMGKKITVILLDNMTNGCINNLQMEHGM
HCCHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCEEEEEEECCCCCHHHHHCCHHCCC
DSYFTEFRFHQQESGRQEGGFIPVDFARIAEGYGCKSYRVTTIEQLHEALEDARKQAVST
HHHHHHHHHHHHHCCCCCCCEECEEHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
LIDIKVLPKTMVHKYLSWWRVGGAQVSRSERIQAVARMLEEHIGQARQY
HHHHHHCCHHHHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; Decarboxylation [C]

Inhibitor: 2-oxobutanoate; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA