| Definition | Escherichia coli ED1a chromosome, complete genome. |
|---|---|
| Accession | NC_011745 |
| Length | 5,209,548 |
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The map label for this gene is dkgB [H]
Identifier: 218688077
GI number: 218688077
Start: 233893
End: 234696
Strand: Direct
Name: dkgB [H]
Synonym: ECED1_0212
Alternate gene names: 218688077
Gene position: 233893-234696 (Clockwise)
Preceding gene: 218688076
Following gene: 218688079
Centisome position: 4.49
GC content: 46.27
Gene sequence:
>804_bases ATGGCTATCCCTGCATTTGGTTTAGGCACTTTCCGTCTGAAAGACGACGTTGTTATTTCATCTGTGAAAACGGCGCTTGA ACTTGATTATCGCGCAATTGATACCGCACAAATCTATGATAACGAAGCCGCAGTAGGTCAGGCGATTGCAGAAAGTGGCG TGCCACGTCATGAACTCTACATCACCACTAAAATCTGGATTGAAAATCTCAGCAAAGACAAATTGATCCCGAGTCTGAAA GAGAGCCTGCAAAAATTGCGTACCGATTATGTTGATCTGACGCTAATCCACTGGCCGTCACCAAGCGATGAAGTCTCTGC TGAAGAGTTTATGCAGGCGCTGCTGGAAGCCAAAAAACAAGGGCTGACGCGTGAGATCGGTATTTCCAACTTCACGATCC CATTGATGGAAAAAGCGATTGCTGCTGTTGGCGCTGAAAACATCGCTACTAACCAGATTGAACTCTCTCCTTATCTGCAA AACCGTAAAGTGGTCGCTTGGGCTAAACAGCACGGTATCCATATTACTTCCTATATGACTCTGGCGTATGGCAATGCACT GAAAGATAAGGTTATTGCTCGTATTGCAGCTAAACACAATGCGACTCCGGCTCAAGTGATTCTGGCGTGGGCTATGGGGG AAGGTTACTCAGTAATTCCTTCTTCTACTAAACGTGAAAACCTGGAGAGTAATCTTAAGGCACAAAATTTACAGCTTGAT GCCGAAGATAAAAAAGCGATCGCCGCACTGGATTGCAACGACCGCCTGGTTAGCCCGGAAGGTCTGGCTCCTGAATGGGA TTAA
Upstream 100 bases:
>100_bases AAAATCGCAAAAATCCTCTGCGTTTTACGCCATTTTTCCACAACTGTCTGAAGCCCATAATCACCTCAGTTAACGAAAAT AGCATTAAAAGAGGCATATT
Downstream 100 bases:
>100_bases GCCTCTCTGACAGCTCCTCCGGGAGCTGTTTTTACATGTCCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTAC CGCACGGTCGCTGTAATAGA
Product: 2,5-diketo-D-gluconate reductase B
Products: NA
Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]
Number of amino acids: Translated: 267; Mature: 266
Protein sequence:
>267_residues MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLK ESLQKLRTDYVDLTLIHWPSPSDEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD AEDKKAIAALDCNDRLVSPEGLAPEWD
Sequences:
>Translated_267_residues MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLK ESLQKLRTDYVDLTLIHWPSPSDEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ NRKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD AEDKKAIAALDCNDRLVSPEGLAPEWD >Mature_266_residues AIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAAVGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKE SLQKLRTDYVDLTLIHWPSPSDEVSAEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQN RKVVAWAKQHGIHITSYMTLAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLDA EDKKAIAALDCNDRLVSPEGLAPEWD
Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]
COG id: COG0656
COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the aldo/keto reductase family [H]
Homologues:
Organism=Homo sapiens, GI310109922, Length=275, Percent_Identity=31.2727272727273, Blast_Score=138, Evalue=6e-33, Organism=Homo sapiens, GI24497577, Length=289, Percent_Identity=29.757785467128, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI5174391, Length=289, Percent_Identity=29.757785467128, Blast_Score=136, Evalue=2e-32, Organism=Homo sapiens, GI310109920, Length=292, Percent_Identity=29.7945205479452, Blast_Score=135, Evalue=3e-32, Organism=Homo sapiens, GI45446745, Length=292, Percent_Identity=29.7945205479452, Blast_Score=135, Evalue=3e-32, Organism=Homo sapiens, GI4503285, Length=292, Percent_Identity=29.7945205479452, Blast_Score=135, Evalue=3e-32, Organism=Homo sapiens, GI5174695, Length=293, Percent_Identity=31.0580204778157, Blast_Score=135, Evalue=4e-32, Organism=Homo sapiens, GI5453543, Length=292, Percent_Identity=29.4520547945205, Blast_Score=134, Evalue=1e-31, Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=30.3030303030303, Blast_Score=131, Evalue=7e-31, Organism=Homo sapiens, GI24497585, Length=291, Percent_Identity=29.2096219931272, Blast_Score=131, Evalue=8e-31, Organism=Homo sapiens, GI223468663, Length=288, Percent_Identity=30.2083333333333, Blast_Score=129, Evalue=3e-30, Organism=Homo sapiens, GI93277124, Length=299, Percent_Identity=28.7625418060201, Blast_Score=128, Evalue=7e-30, Organism=Homo sapiens, GI4502049, Length=288, Percent_Identity=32.9861111111111, Blast_Score=124, Evalue=6e-29, Organism=Homo sapiens, GI300116273, Length=269, Percent_Identity=30.4832713754647, Blast_Score=123, Evalue=2e-28, Organism=Homo sapiens, GI291291012, Length=273, Percent_Identity=29.3040293040293, Blast_Score=122, Evalue=5e-28, Organism=Homo sapiens, GI300116271, Length=278, Percent_Identity=30.5755395683453, Blast_Score=115, Evalue=3e-26, Organism=Homo sapiens, GI310109926, Length=104, Percent_Identity=36.5384615384615, Blast_Score=75, Evalue=8e-14, Organism=Homo sapiens, GI310109924, Length=155, Percent_Identity=29.0322580645161, Blast_Score=75, Evalue=9e-14, Organism=Homo sapiens, GI207028673, Length=104, Percent_Identity=36.5384615384615, Blast_Score=75, Evalue=9e-14, Organism=Escherichia coli, GI1786400, Length=267, Percent_Identity=97.3782771535581, Blast_Score=531, Evalue=1e-152, Organism=Escherichia coli, GI87082198, Length=256, Percent_Identity=37.5, Blast_Score=181, Evalue=6e-47, Organism=Escherichia coli, GI1788081, Length=269, Percent_Identity=28.996282527881, Blast_Score=105, Evalue=4e-24, Organism=Escherichia coli, GI48994888, Length=271, Percent_Identity=29.1512915129151, Blast_Score=75, Evalue=3e-15, Organism=Escherichia coli, GI1787674, Length=257, Percent_Identity=27.2373540856031, Blast_Score=68, Evalue=7e-13, Organism=Caenorhabditis elegans, GI17550248, Length=271, Percent_Identity=34.3173431734317, Blast_Score=146, Evalue=1e-35, Organism=Caenorhabditis elegans, GI17537075, Length=290, Percent_Identity=35.1724137931034, Blast_Score=143, Evalue=1e-34, Organism=Caenorhabditis elegans, GI17537077, Length=288, Percent_Identity=35.0694444444444, Blast_Score=141, Evalue=4e-34, Organism=Caenorhabditis elegans, GI71998625, Length=264, Percent_Identity=29.9242424242424, Blast_Score=132, Evalue=2e-31, Organism=Caenorhabditis elegans, GI17561298, Length=276, Percent_Identity=30.0724637681159, Blast_Score=130, Evalue=5e-31, Organism=Caenorhabditis elegans, GI17552492, Length=266, Percent_Identity=30.0751879699248, Blast_Score=130, Evalue=9e-31, Organism=Caenorhabditis elegans, GI17561300, Length=277, Percent_Identity=31.4079422382672, Blast_Score=129, Evalue=1e-30, Organism=Caenorhabditis elegans, GI17538386, Length=281, Percent_Identity=30.9608540925267, Blast_Score=123, Evalue=9e-29, Organism=Caenorhabditis elegans, GI17566692, Length=262, Percent_Identity=30.9160305343511, Blast_Score=119, Evalue=1e-27, Organism=Caenorhabditis elegans, GI17537079, Length=294, Percent_Identity=26.1904761904762, Blast_Score=115, Evalue=3e-26, Organism=Caenorhabditis elegans, GI17562292, Length=269, Percent_Identity=29.368029739777, Blast_Score=113, Evalue=1e-25, Organism=Caenorhabditis elegans, GI17564128, Length=292, Percent_Identity=25.6849315068493, Blast_Score=112, Evalue=3e-25, Organism=Saccharomyces cerevisiae, GI6324694, Length=273, Percent_Identity=35.5311355311355, Blast_Score=159, Evalue=3e-40, Organism=Saccharomyces cerevisiae, GI6320576, Length=279, Percent_Identity=35.84229390681, Blast_Score=157, Evalue=1e-39, Organism=Saccharomyces cerevisiae, GI6321896, Length=306, Percent_Identity=28.4313725490196, Blast_Score=124, Evalue=1e-29, Organism=Saccharomyces cerevisiae, GI6322556, Length=259, Percent_Identity=26.2548262548263, Blast_Score=114, Evalue=2e-26, Organism=Saccharomyces cerevisiae, GI6319625, Length=275, Percent_Identity=30.5454545454545, Blast_Score=108, Evalue=1e-24, Organism=Saccharomyces cerevisiae, GI6320079, Length=234, Percent_Identity=29.4871794871795, Blast_Score=91, Evalue=1e-19, Organism=Drosophila melanogaster, GI24662789, Length=290, Percent_Identity=31.3793103448276, Blast_Score=139, Evalue=1e-33, Organism=Drosophila melanogaster, GI24644950, Length=294, Percent_Identity=33.3333333333333, Blast_Score=137, Evalue=6e-33, Organism=Drosophila melanogaster, GI24657054, Length=284, Percent_Identity=31.3380281690141, Blast_Score=133, Evalue=1e-31, Organism=Drosophila melanogaster, GI221378297, Length=317, Percent_Identity=31.5457413249211, Blast_Score=132, Evalue=3e-31, Organism=Drosophila melanogaster, GI21356425, Length=286, Percent_Identity=30.7692307692308, Blast_Score=127, Evalue=7e-30, Organism=Drosophila melanogaster, GI20129731, Length=286, Percent_Identity=30.0699300699301, Blast_Score=125, Evalue=4e-29, Organism=Drosophila melanogaster, GI24662785, Length=289, Percent_Identity=33.9100346020761, Blast_Score=124, Evalue=5e-29, Organism=Drosophila melanogaster, GI24663317, Length=285, Percent_Identity=29.1228070175439, Blast_Score=123, Evalue=1e-28, Organism=Drosophila melanogaster, GI24662781, Length=289, Percent_Identity=33.2179930795848, Blast_Score=120, Evalue=9e-28, Organism=Drosophila melanogaster, GI45553081, Length=284, Percent_Identity=27.8169014084507, Blast_Score=111, Evalue=4e-25, Organism=Drosophila melanogaster, GI281366140, Length=259, Percent_Identity=26.2548262548263, Blast_Score=92, Evalue=4e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001395 - InterPro: IPR018170 - InterPro: IPR020471 - InterPro: IPR023210 [H]
Pfam domain/function: PF00248 Aldo_ket_red [H]
EC number: =1.1.1.274 [H]
Molecular weight: Translated: 29441; Mature: 29310
Theoretical pI: Translated: 5.19; Mature: 5.19
Prosite motif: PS00062 ALDOKETO_REDUCTASE_2
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.2 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 1.5 %Met (Mature Protein) 1.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MAIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAAVGQAIAESGVPRHELY CCCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEE ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPSDEVSAEEFMQALLEAKKQ EEEEHEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEEEEH LAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD HHHCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCCHHHHHHHCCCCCCEEEC AEDKKAIAALDCNDRLVSPEGLAPEWD CCCCCEEEEECCCCCEECCCCCCCCCC >Mature Secondary Structure AIPAFGLGTFRLKDDVVISSVKTALELDYRAIDTAQIYDNEAAVGQAIAESGVPRHELY CCCCCCCCEEEECCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHCCCCCCEEE ITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPSDEVSAEEFMQALLEAKKQ EEEEHEECCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHC GLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQNRKVVAWAKQHGIHITSYMT CCEEECCCCCCCHHHHHHHHHHHCCCCCCCCEEEECHHHCCCEEEEEHHHCCEEEEEEEH LAYGNALKDKVIARIAAKHNATPAQVILAWAMGEGYSVIPSSTKRENLESNLKAQNLQLD HHHCCHHHHHHHHHHHHHCCCCHHHEEEEEECCCCCEECCCCCHHHHHHHCCCCCCEEEC AEDKKAIAALDCNDRLVSPEGLAPEWD CCCCCEEEEECCCCCEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 10.0
TargetDB status: NA
Availability: NA
References: 11206551; 11258796; 11108008 [H]