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The map label for this gene is lpxD

Identifier: 218688054

GI number: 218688054

Start: 205746

End: 206771

Strand: Direct

Name: lpxD

Synonym: ECED1_0185

Alternate gene names: 218688054

Gene position: 205746-206771 (Clockwise)

Preceding gene: 218688053

Following gene: 218688055

Centisome position: 3.95

GC content: 52.34

Gene sequence:

>1026_bases
ATGCCTTCAATTCGACTGGCTGATTTAGCGCAGCAGTTGGATGCAGAACTACACGGTGATGGCGATATCGTCATCACCGG
CGTTGCGTCCATGCAATCTGCACAAACAGGTCACATTACGTTCATGGTTAACCCCAAATACCGTGAGCATTTAGGCTTGT
GCCAGGCGTCCGCGGTTGTCATGACCCAGGACGATCTTCCTTTCGCGAAAAGTGCCGCGCTGGTAGTGAAGAATCCCTAC
CTGACTTACGCGCGTATGGCGCAAATTTTAGATACCACGCCGCAGCCCGCGCAGAACATTGCACCCAGTGCAGTGATTGA
CGCGACGGCGAAGCTGGGTAACAACGTATCGATTGGCGCTAACGCGGTGATTGAGTCCGGCGTTGAACTGGGCGATAACG
TGATTATCGGTGCCGGTTGCTTCGTAGGTAAAAACAGCAAAATCGGTGCAGGTTCGCGTCTCTGGGCGAACGTAACCATT
TACCATGAGATCCAGATCGGTCAGAATTGCCTGATCCAGTCCGGAACAGTGGTAGGCGCAGACGGCTTTGGTTATGCCAA
CGATCGTGGTAACTGGGTGAAGATCCCACAGATTGGTCGCGTAATTATTGGCGATCGCGTGGAGATCGGTGCCTGCACTA
CCATTGATCGTGGCGCGCTGGATGACACTGTTATTGGCAATGGTGTGATCATTGATAACCAGTGCCAGATTGCACATAAC
GTCGTGATTGGCGACAATACGGCAGTTGCCGGTGGCGTCATTATGGCGGGCAGCCTGAAAATTGGTCGTTACTGCATGAT
CGGCGGAGCTAGCGTAATCAACGGGCATATGGAAATATGCGACAAAGTGACGGTTACGGGCATGGGTATGGTGATGCGTC
CCATCACTGAACCAGGCGTCTATTCCTCAGGCATTCCGCTGCAACCCAACAAAGTCTGGCGCAAAACCGCTGCACTGGTG
ATGAACATTGATGACATGAGCAAGCGTCTGAAATCGCTTGAGCGCAAGGTTAATCAACAAGACTAA

Upstream 100 bases:

>100_bases
CCAGGATATCGATCTGGTTGTTGATGCAAATGCCGTTGCTTACAACAGCAGCGATGTAAAAGACATCACTGCCGACGTAC
TGAAACAGGTTAAATAAGTA

Downstream 100 bases:

>100_bases
CGTTCCATCTTTTGTTCGCCAAACTTTACGGCCTGTCTCATTCTTACGATTGCGGCAGGCCGTGTTATTATTGTCGTTTC
TTATATTTTGACAGGAAGAG

Product: UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 341; Mature: 340

Protein sequence:

>341_residues
MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPY
LTYARMAQILDTTPQPAQNIAPSAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTVIGNGVIIDNQCQIAHN
VVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALV
MNIDDMSKRLKSLERKVNQQD

Sequences:

>Translated_341_residues
MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPY
LTYARMAQILDTTPQPAQNIAPSAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTVIGNGVIIDNQCQIAHN
VVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALV
MNIDDMSKRLKSLERKVNQQD
>Mature_340_residues
PSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYL
TYARMAQILDTTPQPAQNIAPSAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTIY
HEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTVIGNGVIIDNQCQIAHNV
VIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVM
NIDDMSKRLKSLERKVNQQD

Specific function: Lipid A biosynthesis; third step. [C]

COG id: COG1044

COG function: function code M; UDP-3-O-[3-hydroxymyristoyl] glucosamine N-acyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family. LpxD subfamily

Homologues:

Organism=Escherichia coli, GI1786376, Length=341, Percent_Identity=99.7067448680352, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1786378, Length=217, Percent_Identity=27.6497695852535, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LPXD_ECO57 (P65323)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E85502
- PIR:   E90651
- RefSeq:   NP_285873.1
- RefSeq:   NP_308208.1
- ProteinModelPortal:   P65323
- SMR:   P65323
- EnsemblBacteria:   EBESCT00000025922
- EnsemblBacteria:   EBESCT00000057533
- GeneID:   913891
- GeneID:   956910
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z0191
- KEGG:   ecs:ECs0181
- GeneTree:   EBGT00050000010008
- HOGENOM:   HBG469615
- OMA:   CFVGKNT
- ProtClustDB:   PRK00892
- BioCyc:   ECOL83334:ECS0181-MONOMER
- HAMAP:   MF_00523
- InterPro:   IPR001451
- InterPro:   IPR018357
- InterPro:   IPR011004
- InterPro:   IPR007691
- InterPro:   IPR020573
- TIGRFAMs:   TIGR01853

Pfam domain/function: PF00132 Hexapep; PF04613 LpxD; SSF51161 Trimer_LpxA_like

EC number: 2.3.1.-

Molecular weight: Translated: 36025; Mature: 35894

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVV
CCCCHHHHHHHHHCHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHHHCCCCCCEEE
MTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAPSAVIDATAKLGNNVSIGA
EEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCEEECC
NAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTIYHEIQIGQNCLIQSGTVVGA
HHHHHCCHHCCCCEEEECEEEECCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCEEEC
DGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTVIGNGVIIDNQCQIAHN
CCCCCCCCCCCEEECCCCCEEEECCEEEECEECCCCCCCCCCEEECCCEEECCCCEEEEE
VVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGV
EEECCCCEEECCEEEECCEEECCEEEECCHHHCCCHHHHHCEEEEECCCEEEECCCCCCC
YSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLERKVNQQD
CCCCCCCCCHHHHHHEEHEEEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVNPKYREHLGLCQASAVV
CCCHHHHHHHHHCHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCHHHHHCCCCCCEEE
MTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAPSAVIDATAKLGNNVSIGA
EEECCCCCCCCCEEEEECCCHHHHHHHHHHCCCCCCHHHCCCHHHHHHHHHCCCCEEECC
NAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTIYHEIQIGQNCLIQSGTVVGA
HHHHHCCHHCCCCEEEECEEEECCCCCCCCCCEEEEEEEEEEEEECCCCEEEECCCEEEC
DGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRGALDDTVIGNGVIIDNQCQIAHN
CCCCCCCCCCCEEECCCCCEEEECCEEEECEECCCCCCCCCCEEECCCEEECCCCEEEEE
VVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGHMEICDKVTVTGMGMVMRPITEPGV
EEECCCCEEECCEEEECCEEECCEEEECCHHHCCCHHHHHCEEEEECCCEEEECCCCCCC
YSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLERKVNQQD
CCCCCCCCCHHHHHHEEHEEEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases; Transferring groups other than amino-acyl groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796