Click here to switch to the map view.

The map label for this gene is ampD [H]

Identifier: 218687984

GI number: 218687984

Start: 120918

End: 121469

Strand: Direct

Name: ampD [H]

Synonym: ECED1_0108

Alternate gene names: 218687984

Gene position: 120918-121469 (Clockwise)

Preceding gene: 218687979

Following gene: 218687985

Centisome position: 2.32

GC content: 54.17

Gene sequence:

>552_bases
ATGTTGTTAGAACAGGGGTGGCTGGTTGGCGCGCGCCGCGTTCCCTCACCACATTACGATTGCCGCCCGGATGACGAAAC
ACCCACCCTGCTGGTGGTGCACAATATTAGCCTGCCGCCAGGCGAGTTTGGCGGTCCGTGGATCGACGCATTATTCACTG
GAACTATTGATCCGCAGGCACATTCTTTCTTTGCTGAGATCGCCCATTTGCGCGTCTCCGCTCACTGTTTGATTCGCCGC
GATGGTGAAATAGTCCAGTATGTTCCTTTCGATAAACGTGCCTGGCATGCGGGAGTCTCTCAGTATCAGGGGCGTGAACG
CTGCAATGATTTTTCTATTGGGATTGAGCTGGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTG
CGGCGGTTACGCGGGCACTGATTGATCGCTATCCAGATATCGCTAATAACATGACGGGCCATTGTGATATTGCGCCGGAT
CGGAAAACCGATCCCGGCCCTGCATTTGATTGGGCACGGTTTCGTGCACTGGTCAGCAAGGAGACAACATGA

Upstream 100 bases:

>100_bases
GGCGAGGCGGCATGTTAAAACTCCAGATAGCTAACGAATCATAAGGTAGAAACATGCTACTCTGAACCGGGTATTAGCAC
CACATATAAGGAGATCCTGC

Downstream 100 bases:

>100_bases
CGCTATTTACAACCTTACTGGTGTTAATTTTCGAGCGCCTGTTTAAGTTGGGCGAGCACTGGCAGCTTGATCATCGTCTT
GAAGCGTTCTTTCGGCGGGT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHSFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
RKTDPGPAFDWARFRALVSKETT

Sequences:

>Translated_183_residues
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHSFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
RKTDPGPAFDWARFRALVSKETT
>Mature_183_residues
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQAHSFFAEIAHLRVSAHCLIRR
DGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
RKTDPGPAFDWARFRALVSKETT

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=183, Percent_Identity=97.8142076502732, Blast_Score=372, Evalue=1e-105,
Organism=Escherichia coli, GI1787092, Length=129, Percent_Identity=40.3100775193798, Blast_Score=82, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 20537; Mature: 20537

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQA
CCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHEECCCCCHHH
HSFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGT
HHHHHHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHHHHHHCCHHHCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPDRKTDPGPAFDWARFRALVSK
CEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHC
ETT
CCC
>Mature Secondary Structure
MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFGGPWIDALFTGTIDPQA
CCCCCCCEECCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHEECCCCCHHH
HSFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQYQGRERCNDFSIGIELEGT
HHHHHHHHHHHHHEEEEEECCCCEEEECCCCCHHHHHHHHHHCCHHHCCCEEEEEEECCC
DTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPDRKTDPGPAFDWARFRALVSK
CEEEECCHHHHHHHHHHHHHHHHCCHHHCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHC
ETT
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2691840; 2607970; 9278503; 7959070 [H]