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The map label for this gene is ftsI [H]

Identifier: 218687961

GI number: 218687961

Start: 93600

End: 95366

Strand: Direct

Name: ftsI [H]

Synonym: ECED1_0085

Alternate gene names: 218687961

Gene position: 93600-95366 (Clockwise)

Preceding gene: 218687960

Following gene: 218687962

Centisome position: 1.8

GC content: 53.71

Gene sequence:

>1767_bases
ATGAAAGCAGCGGCGAAAACGCAGAAACCAAAACGTCAGGAAGAACATGCCAACTTTATCAGTTGGCGTTTTGCGTTGTT
ATGCGGCTGTATTCTCCTGGCGCTGGCTTTTCTGCTCGGACGCGTAGCGTGGTTACAAGTTATCTCCCCGGATATGCTGG
TGAAAGAGGGCGACATGCGTTCTCTTCGCGTTCAGCAAGTTTCCACCTCCCGCGGCATGATTACTGACCGTTCTGGTCGC
CCGTTAGCGGTGAGCGTGCCGGTTAAAGCGATTTGGGCTGACCCGAAAGAAGTGCATGACGCTGGCGGTATCAGCGTCGG
TGAACGCTGGAAGGCGCTGGCTAACGCGCTCAATATTCCGCTGGATCAGCTCTCAGCCCGCATTAACGCCAACCCGAAAG
GGCGCTTTATTTATCTGGCGCGTCAGGTAAACCCTGACATGGCGGACTACATCAAAAAACTGAAACTGCCGGGGATTCAT
CTGCGTGAAGAATCTCGCCGTTATTATCCATCCGGCGAAGTGACTGCTCACCTCATCGGTTTTACTAACGTCGATAGCCA
AGGGATTGAGGGTGTCGAGAAGAGTTTCGATAAATGGCTTACCGGGCAGCCGGGTGAGCGCATTGTGCGTAAAGACCGCT
ATGGTCGCGTAATTGAAGATATTTCTTCTACTGACAGCCAGGCAGCGCACAACCTGACGCTGAGTATTGATGAACGCCTG
CAGGCGCTGGTTTATCGCGAACTGAACAACGCAGTGGCCTTTAACAAGGCTGAATCTGGTAGCGCCGTGCTGGTGGATGT
CAACACCGGTGAAGTGCTGGCGATGGCTAACAGCCCGTCATACAACCCAAATAATCTGAGCGGCACGCCGAAAGAAGCGA
TGCGTAACCGTACCATCACCGACGTGTTTGAACCGGGCTCAACGGTTAAACCGATGGTGGTGATGACCGCGTTGCAACGT
GGCGTGGTGCGGGAAAACTCGGTACTCAATACCGTTCCTTATCGAATTAACGGCCACGAAATCAAAGACGTGGCGCGCTA
CAGCGAATTAACCCTGACCGGGGTATTACAGAAGTCGAGTAACGTCGGTGTTTCCAAACTGGCGTTAGCGATGCCGTCCT
CAGCGTTAGTAGATACTTACTCACGTTTTGGACTGGGAAAAGCGACCAATTTGGGGTTGGTCGGAGAACGCAGTGGCTTA
TATCCTCAAAAACAACGGTGGTCTGACATAGAGAGGGCCACCTTCTCTTTCGGCTACGGGCTAATGGTAACACCGTTACA
GTTAGCGCGAGTCTACGCAACTATCGGCAGCTACGGCATTTATCGCCCACTGTCGATTACCAAAGTTGACCCCCCGGTTC
CCGGTGAACGTGTCTTCCCGGAATCCATTGTTCGCACCGTGGTGCATATGATGGAAAGCGTGGCGCTACCGGGCGGCGGC
GGCGTGAAGGCGGCGATTAAAGGCTATCGTATCGCCATTAAAACCGGTACCGCGAAAAAGGTCGGGCCGGACGGTCGCTA
CATCAATAAATATATTGCTTATACCGCAGGCGTTGCGCCTGCGAGTCAGCCGCGCTTCGCGCTGGTTGTTGTTATCAACG
ATCCGCAGGCGGGTAAATACTACGGCGGCGCCGTTTCCGCGCCGGTCTTTGGTGCCATCATGGGCGGCGTATTGCGTACC
ATGAACATCGAGCCGGATGCGCTGACAACGGGCGATAAAAATGAATTTGTGATTAATCAAGGCGAGGGTACAGGTGGCAG
ATCGTAA

Upstream 100 bases:

>100_bases
TAGCCGGGTGGAAAGGATAGCCACGGAAAAGCTGCAAATGCAGCATGTTGATCCGTCACAAGAAAATATCGTAGTGCAAA
AATAAGGATAAACGCGACGC

Downstream 100 bases:

>100_bases
TTTGCGCGACCTTCTTGCTCCGTGGGTGCCAGACGCACCTTCGCGAGCACTGCGAGAGATGACACTCGACAGCCGTGTGG
CTGCGGCGGGCGATCTCTTT

Product: transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3)

Products: NA

Alternate protein names: Penicillin-binding protein 3; PBP-3; Peptidoglycan glycosyltransferase 3 [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGR
PLAVSVPVKAIWADPKEVHDAGGISVGERWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLTLSIDERL
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQR
GVVRENSVLNTVPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGL
YPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRT
MNIEPDALTTGDKNEFVINQGEGTGGRS

Sequences:

>Translated_588_residues
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGR
PLAVSVPVKAIWADPKEVHDAGGISVGERWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLTLSIDERL
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQR
GVVRENSVLNTVPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGL
YPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRT
MNIEPDALTTGDKNEFVINQGEGTGGRS
>Mature_588_residues
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGR
PLAVSVPVKAIWADPKEVHDAGGISVGERWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLTLSIDERL
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQR
GVVRENSVLNTVPYRINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGL
YPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRT
MNIEPDALTTGDKNEFVINQGEGTGGRS

Specific function: Cell wall formation. Essential for the formation of a septum of the murein sacculus. Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein; Periplasmic side. Note=The bulk of the molecule, except for the N-terminal membrane anchor region, protrudes into the periplasmic space, where it acts on murein (By similarity) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=588, Percent_Identity=99.4897959183673, Blast_Score=1198, Evalue=0.0,
Organism=Escherichia coli, GI1786854, Length=636, Percent_Identity=25, Blast_Score=139, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 63908; Mature: 63908

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCH
SLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGERWKALANALNIP
HHHHHHHHCCCCEEECCCCCCEEEECCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHCCC
LDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIG
HHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEE
FTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLTLSIDERL
EECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEHHHHH
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTIT
HHHHHHHHHCCEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCH
DVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPYRINGHEIKDVARYSELTLTGVLQKSS
HHCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCHHHHHHHHHHHEEEEEECCCC
NVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYG
CCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCC
LMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKY
CCEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
YGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFVINQGEGTGGRS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAWLQVISPDMLVKEGDMR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCH
SLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAGGISVGERWKALANALNIP
HHHHHHHHCCCCEEECCCCCCEEEECCCCEEECCHHHHHCCCCCCHHHHHHHHHHHHCCC
LDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIHLREESRRYYPSGEVTAHLIG
HHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCEECCCCCCCCCCCCCEEEEEE
FTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVIEDISSTDSQAAHNLTLSIDERL
EECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHCEEEEEHHHHH
QALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMANSPSYNPNNLSGTPKEAMRNRTIT
HHHHHHHHHCCEEECCCCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCHHHHHHCCCCH
DVFEPGSTVKPMVVMTALQRGVVRENSVLNTVPYRINGHEIKDVARYSELTLTGVLQKSS
HHCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCEEECCCHHHHHHHHHHHEEEEEECCCC
NVGVSKLALAMPSSALVDTYSRFGLGKATNLGLVGERSGLYPQKQRWSDIERATFSFGYG
CCCHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHCCCC
LMVTPLQLARVYATIGSYGIYRPLSITKVDPPVPGERVFPESIVRTVVHMMESVALPGGG
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
GVKAAIKGYRIAIKTGTAKKVGPDGRYINKYIAYTAGVAPASQPRFALVVVINDPQAGKY
CCEEEECCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCC
YGGAVSAPVFGAIMGGVLRTMNIEPDALTTGDKNEFVINQGEGTGGRS
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]