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The map label for this gene is rihC [H]

Identifier: 218687904

GI number: 218687904

Start: 28515

End: 29429

Strand: Direct

Name: rihC [H]

Synonym: ECED1_0027

Alternate gene names: 218687904

Gene position: 28515-29429 (Clockwise)

Preceding gene: 218687903

Following gene: 218687905

Centisome position: 0.55

GC content: 55.08

Gene sequence:

>915_bases
ATGCGTTTACCTATCTTCCTCGATACAGACCCCGGCATTGACGATGCCGTTGCCATTGCCGCCGCGATTTTTGCCCCCGA
ACTCGATCTGCAACTGATGACCACTGTCGCGGGTAATGTTTCGGTAGAGAAAACCACCCGCAACGCCCTGCAACTGCTGC
ATTTCTGGAATGTGGATATTCCGCTTGCCCAGGGCGCTGCCGTACCACTGGTTCGTGCGCCGCGCGATGCGGCATCTGTA
CACGGTGAATCGGGAATGGCTGGCTATGACTTTGTTGAACACAACCGTCAGCCACTGGGTATACCGGCGTTTCTGGCAAT
TCGTGATGCCCTGATGCGTGCGCCAGAGCCCGTTACCCTGGTGGCCATCGGCCCGTTAACCAATATTGCGCTGTTACTTT
CACAATGCCCGGAATGTAAGCCGTATATTCGCCGTCTGGTGATCATGGGCGGTTCTGCTGGTCGCGGCAACTGCACACCA
AACGCCGAGTTTAATATCGCTGCCGATCCAGAAGCGGCAGCCTGTGTCTTCCGTAGCGGCATCGAAATCGTCATGTGCGG
TCTGGATGTCACCAATCAGGCAATATTAACCCCTGACTATCTCGCTACTTTGCCGGAATTGAATCGCACCGGAAAAATGC
TACACGCCCTGTTTAGCCACTACCGTAGCGGCAGTATGCAAAGTGGACTACGTATGCACGATCTTTGCGCTATCGCCTGG
CTGGTGCGCCCGGAATTGTTCACTCTCAAACCGTGTTTTGTCGCGGTAGAAACTCAGGGTGAATTTACCTCAGGCACGAC
GGTTGTTGATATCGACGGTTGCCTGGGCAAACCAGCCAATGTACAGGTGGCGCTGGATCTGGATGTGAAAGGCTTCCAGC
AATGGGTGGCTGAGGTGCTGGCCCTGGCGCTCTAA

Upstream 100 bases:

>100_bases
AAGAGCTGCGTGTCGATATTCGTGAAGTCGATTAAGTCATTAGCAGCCTAAGTTATGCGAAAATGCCGGTCTTGTTACCG
GCATTTTTTATGGAGAAAAC

Downstream 100 bases:

>100_bases
TAATAAAACAAGGAAGTGTTCTATGACGTATGGAGAAGCGTATCTGGAAGGGTGGAAAAACATCTTTAATTATGAAGGTG
TTTCCAATCGCTTCGAGTTC

Product: ribonucleoside hydrolase RihC

Products: uracil; ribose; xanthine; hypoxanthine; adenine; cytosine; guanine [C]

Alternate protein names: Purine/pyrimidine ribonucleoside hydrolase [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRQPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLDVKGFQQWVAEVLALAL

Sequences:

>Translated_304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRQPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLDVKGFQQWVAEVLALAL
>Mature_304_residues
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNVDIPLAQGAAVPLVRAPRDAASV
HGESGMAGYDFVEHNRQPLGIPAFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLDVKGFQQWVAEVLALAL

Specific function: Hydrolyzes both purine and pyrimidine ribonucleosides with a broad-substrate specificity [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=98.019801980198, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI1786871, Length=309, Percent_Identity=45.6310679611651, Blast_Score=235, Evalue=2e-63,
Organism=Escherichia coli, GI1788486, Length=305, Percent_Identity=39.344262295082, Blast_Score=201, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI17565698, Length=188, Percent_Identity=33.5106382978723, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32565474, Length=189, Percent_Identity=31.7460317460317, Blast_Score=71, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6320608, Length=342, Percent_Identity=29.2397660818713, Blast_Score=110, Evalue=4e-25,
Organism=Drosophila melanogaster, GI24641839, Length=196, Percent_Identity=33.1632653061224, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24583915, Length=191, Percent_Identity=34.0314136125654, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24641837, Length=328, Percent_Identity=30.4878048780488, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022976 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 32600; Mature: 32600

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNVDI
CCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCC
IEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
CEEEEECCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLDVKGFQQWVAEVL
HHCCCCEEECCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALAL
HHCC
>Mature Secondary Structure
MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEKTTRNALQLLHFWNVDI
CCCCEEECCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHCCCC
PLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRQPLGIPAFLAIRDALMRAPEPVTL
CCCCCCCCCEEECCCHHHHCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEE
VAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTPNAEFNIAADPEAAACVFRSG
EEECCHHHHHHHHHCCCCHHHHHHEEEEECCCCCCCCCCCCCCEEEECCCHHHHHHHHCC
IEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALFSHYRSGSMQSGLRMHDLCAIAW
CEEEEECCCCCCCEEECCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHCCCHHHHHHHHHH
LVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLGKPANVQVALDLDVKGFQQWVAEVL
HHCCCCEEECCEEEEEECCCCCCCCCEEEEECCCCCCCCCEEEEEECCHHHHHHHHHHHH
ALAL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; xanthosine; inosine; adenosine; cytidine; guanosine [C]

Specific reaction: uridine + H2O = uracil + ribose xanthosine + H2O = xanthine + ribose inosine + H2O = hypoxanthine + ribose adenosine + H2O = adenine + ribose cytidine + H2O = cytosine + ribose guanosine + H2O = guanine + ribose [C]

General reaction: Nonspecific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA