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The map label for this gene is nifA [H]

Identifier: 218550821

GI number: 218550821

Start: 3615732

End: 3617063

Strand: Reverse

Name: nifA [H]

Synonym: EFER_3536

Alternate gene names: 218550821

Gene position: 3617063-3615732 (Counterclockwise)

Preceding gene: 218550822

Following gene: 218550819

Centisome position: 78.83

GC content: 42.42

Gene sequence:

>1332_bases
TTGCGCTCACAGAGTGTGAAGCAGAAGACAAAAGAAATTCCCGTTGCGACTAAAATAGGGGTATCTCTTTTTCCATTGCA
GAAATCACCACCTCGTTATTGCTCACGACAGGAGGCAAGCGTGGTTACGATCCGCTGGGATATAAAAACAATTGATCGCC
TCTCAATGGTTGAGGATGTTCTCAAGGAGTTTTCCTGTTGCGATCTTAATATAGTTTCCATGAAAGTCACCCCAGGACGA
ATTCTCATAAAATCCTGGTGCCGCCAGGTGCAGGATATTCCTGTCCTCCAGACACGACTGGAACAGAATGCTGATATCGT
TAATGTGGCTTATCTTTCTGAGACGGAAAATAGTCTTTCTTCTCCAGATTCAACGCGCCCACGGTATTTTTCTGACATTA
TTTGTAATAGCCCTGCGATGAAAATCGTTATTGATAAGGCTATAAAAATTGTCGATAGCAAATATACGGTATTAATCCGG
GGTGAAACTGGAACAGGTAAAGATCTCCTGGCGCGAGCAATTCATAATACAGGTTCCCGACGTTTTTATCCTTTTATCCC
TGTTAACTGCTCAGCATTACCAGAATCGCTGATGGAAAGTGAGTTTTTCGGCTATGAAAAAGGGGCATTTAGTGGGGCCG
ATAGCCAGGGTAAAAAAGGCCTTTTTGAGATGGCAGACAAAGGAACACTTTTCCTTGATGAGTTGGGGGAATTACCGCTT
AATTTACAAGGTAAACTTTTACGGGTACTACAGGAAGGTGGGATTCGACGGGTCGGGGGTACGCAAATAATTCCAATTAA
TACGCGTATTATTGTCGCTACTAACGCTAATCTGGAAGAGATGATTGCTCATCGCACCTTGCGTGCCGACCTCTGGTATC
GTATCAATGTGCTTTCTCTGACTATACCGCCATTGCGTGAACGTCCACATGATCTCAACTTATTAATCCGTTATTTTGTA
CAGAAATATGCTAAGGAATTTAAACGGAATATCATTCTTGATAAAACTGCCTACGAAACTTTGCGTCAGCACACGTGGCC
TGGCAATATCAGAGAACTGGAAAATACCATTATCCGCCTGATGCTAATGTGTGAAAACGATATTATTCGCAGTGATGATT
TGGGACTGGATAAGAGTTTGTCTTCAGTATCAATAAACTCGCCAAATTCATTTAAAGATCAGGTGCTTCATACAGAGAGA
CAGTTAATAGAAAAAATGCTGGCTGACAATGTATCCTCGCGAGAAATAGCAAAAACATTAGGTGTTTCACACACGACAGT
ACTCAATAAAATTCATCGCTACCAACAGGGCATCAGCGCCGCCAGTGATTGA

Upstream 100 bases:

>100_bases
CGGTGGTCAACTGGTAGCTTGTTTTGCTGTTGATCAGGATGAAAATCCGCAGCGGTAATGTTCAAAACCGGAGCCTTGTG
CTCCGGTTCCACTTTTTCGT

Downstream 100 bases:

>100_bases
CGGCGCGATTTAATATCACTCAATAAAAGTCAGGAAAGGTGTTAAACAGAGCAGGATACCGTAAAGTAAAATAAGCCAAA
TTTTCAGGCCTCGCAGTTTC

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MRSQSVKQKTKEIPVATKIGVSLFPLQKSPPRYCSRQEASVVTIRWDIKTIDRLSMVEDVLKEFSCCDLNIVSMKVTPGR
ILIKSWCRQVQDIPVLQTRLEQNADIVNVAYLSETENSLSSPDSTRPRYFSDIICNSPAMKIVIDKAIKIVDSKYTVLIR
GETGTGKDLLARAIHNTGSRRFYPFIPVNCSALPESLMESEFFGYEKGAFSGADSQGKKGLFEMADKGTLFLDELGELPL
NLQGKLLRVLQEGGIRRVGGTQIIPINTRIIVATNANLEEMIAHRTLRADLWYRINVLSLTIPPLRERPHDLNLLIRYFV
QKYAKEFKRNIILDKTAYETLRQHTWPGNIRELENTIIRLMLMCENDIIRSDDLGLDKSLSSVSINSPNSFKDQVLHTER
QLIEKMLADNVSSREIAKTLGVSHTTVLNKIHRYQQGISAASD

Sequences:

>Translated_443_residues
MRSQSVKQKTKEIPVATKIGVSLFPLQKSPPRYCSRQEASVVTIRWDIKTIDRLSMVEDVLKEFSCCDLNIVSMKVTPGR
ILIKSWCRQVQDIPVLQTRLEQNADIVNVAYLSETENSLSSPDSTRPRYFSDIICNSPAMKIVIDKAIKIVDSKYTVLIR
GETGTGKDLLARAIHNTGSRRFYPFIPVNCSALPESLMESEFFGYEKGAFSGADSQGKKGLFEMADKGTLFLDELGELPL
NLQGKLLRVLQEGGIRRVGGTQIIPINTRIIVATNANLEEMIAHRTLRADLWYRINVLSLTIPPLRERPHDLNLLIRYFV
QKYAKEFKRNIILDKTAYETLRQHTWPGNIRELENTIIRLMLMCENDIIRSDDLGLDKSLSSVSINSPNSFKDQVLHTER
QLIEKMLADNVSSREIAKTLGVSHTTVLNKIHRYQQGISAASD
>Mature_443_residues
MRSQSVKQKTKEIPVATKIGVSLFPLQKSPPRYCSRQEASVVTIRWDIKTIDRLSMVEDVLKEFSCCDLNIVSMKVTPGR
ILIKSWCRQVQDIPVLQTRLEQNADIVNVAYLSETENSLSSPDSTRPRYFSDIICNSPAMKIVIDKAIKIVDSKYTVLIR
GETGTGKDLLARAIHNTGSRRFYPFIPVNCSALPESLMESEFFGYEKGAFSGADSQGKKGLFEMADKGTLFLDELGELPL
NLQGKLLRVLQEGGIRRVGGTQIIPINTRIIVATNANLEEMIAHRTLRADLWYRINVLSLTIPPLRERPHDLNLLIRYFV
QKYAKEFKRNIILDKTAYETLRQHTWPGNIRELENTIIRLMLMCENDIIRSDDLGLDKSLSSVSINSPNSFKDQVLHTER
QLIEKMLADNVSSREIAKTLGVSHTTVLNKIHRYQQGISAASD

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=322, Percent_Identity=45.0310559006211, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=44.6483180428135, Blast_Score=254, Evalue=8e-69,
Organism=Escherichia coli, GI1789087, Length=316, Percent_Identity=43.0379746835443, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1789233, Length=303, Percent_Identity=39.9339933993399, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI1790437, Length=303, Percent_Identity=37.953795379538, Blast_Score=220, Evalue=1e-58,
Organism=Escherichia coli, GI1787583, Length=312, Percent_Identity=37.8205128205128, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786524, Length=349, Percent_Identity=38.9684813753582, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1790299, Length=245, Percent_Identity=44.4897959183673, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI1788905, Length=257, Percent_Identity=44.7470817120623, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI87082152, Length=236, Percent_Identity=47.4576271186441, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI87081872, Length=233, Percent_Identity=43.3476394849785, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081858, Length=322, Percent_Identity=29.8136645962733, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789828, Length=252, Percent_Identity=32.1428571428571, Blast_Score=128, Evalue=6e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50153; Mature: 50153

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSQSVKQKTKEIPVATKIGVSLFPLQKSPPRYCSRQEASVVTIRWDIKTIDRLSMVEDV
CCCHHHHHHHHHCCCEEECCCEEEECCCCCCHHCCCCCCEEEEEEECHHHHHHHHHHHHH
LKEFSCCDLNIVSMKVTPGRILIKSWCRQVQDIPVLQTRLEQNADIVNVAYLSETENSLS
HHHCCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCHHCC
SPDSTRPRYFSDIICNSPAMKIVIDKAIKIVDSKYTVLIRGETGTGKDLLARAIHNTGSR
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
RFYPFIPVNCSALPESLMESEFFGYEKGAFSGADSQGKKGLFEMADKGTLFLDELGELPL
EEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEEHHHHCCCCC
NLQGKLLRVLQEGGIRRVGGTQIIPINTRIIVATNANLEEMIAHRTLRADLWYRINVLSL
CCHHHHHHHHHHCCCEECCCCEEEEECEEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEE
TIPPLRERPHDLNLLIRYFVQKYAKEFKRNIILDKTAYETLRQHTWPGNIRELENTIIRL
ECCHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCHHHHHHHHHHH
MLMCENDIIRSDDLGLDKSLSSVSINSPNSFKDQVLHTERQLIEKMLADNVSSREIAKTL
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GVSHTTVLNKIHRYQQGISAASD
CCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRSQSVKQKTKEIPVATKIGVSLFPLQKSPPRYCSRQEASVVTIRWDIKTIDRLSMVEDV
CCCHHHHHHHHHCCCEEECCCEEEECCCCCCHHCCCCCCEEEEEEECHHHHHHHHHHHHH
LKEFSCCDLNIVSMKVTPGRILIKSWCRQVQDIPVLQTRLEQNADIVNVAYLSETENSLS
HHHCCCCCEEEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEEEECCCCHHCC
SPDSTRPRYFSDIICNSPAMKIVIDKAIKIVDSKYTVLIRGETGTGKDLLARAIHNTGSR
CCCCCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCC
RFYPFIPVNCSALPESLMESEFFGYEKGAFSGADSQGKKGLFEMADKGTLFLDELGELPL
EEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHCCCCEEHHHHCCCCC
NLQGKLLRVLQEGGIRRVGGTQIIPINTRIIVATNANLEEMIAHRTLRADLWYRINVLSL
CCHHHHHHHHHHCCCEECCCCEEEEECEEEEEEECCCHHHHHHHHHHHHHHEEEEEEEEE
TIPPLRERPHDLNLLIRYFVQKYAKEFKRNIILDKTAYETLRQHTWPGNIRELENTIIRL
ECCHHHCCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHCCCCCCHHHHHHHHHHH
MLMCENDIIRSDDLGLDKSLSSVSINSPNSFKDQVLHTERQLIEKMLADNVSSREIAKTL
HHHHCCCCCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
GVSHTTVLNKIHRYQQGISAASD
CCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1779763; 1362170 [H]