Click here to switch to the map view.

The map label for this gene is gntR

Identifier: 218550703

GI number: 218550703

Start: 3483310

End: 3484305

Strand: Reverse

Name: gntR

Synonym: EFER_3414

Alternate gene names: 218550703

Gene position: 3484305-3483310 (Counterclockwise)

Preceding gene: 218550704

Following gene: 218550702

Centisome position: 75.93

GC content: 54.52

Gene sequence:

>996_bases
ATGAAAAAGAAAAGACCCGTACTTCAGGATGTGGCTGACCGTGTAGGCGTGACCAAAATGACGGTCAGCCGTTTTTTACG
CAACCCGGAGCAGGTTTCCGTCGCTCTACGTGGCAAGATTGCCGCCGCTCTTGATGAACTGGGTTATATTCCCAATCGCG
CGCCCGATATCCTCTCTAACGCCACCAGCCGGGCGATTGGCGTCCTGTTACCTTCACTCACCAACCAGGTTTTCGCTGAA
GTATTACGCGGTATCGAAAGCGTCACCGACGCACACGGTTATCAGACGATGCTGGCGCACTACGGTTATAAACCGGAAAT
GGAGCAAGAACGTCTCGAGTCGATGCTTTCCTGGAATATCGACGGGCTTATTCTCACCGAACGTACCCACACGCCCCGCA
CCTTAAAGATGATTGAAGTAGCGGGTATTCCGGTGGTGGAGCTGATGGACAGTAAGTCGCCGTGCCTCGATATCGCCGTC
GGTTTTGATAACTTTGAAGCGGCACGCCAGATGACCACCGCCATTATTGCCCGCGGGCATCGTCACATTGCCTATCTCGG
CGCACGCCTCGACGAACGTACTATCATCAAACAGAAGGGATATGAACAGGCGATGCTGGATGCAGGCCTGGTGCCGTATA
GCGTGATGGTCGAGCAATCTTCTTCTTACTCTTCCGGTATTGAACTGATTCGCCAGGCGCGGCGGGAATATCCGCAGCTG
GATGGCGTATTTTGTACTAACGATGACCTGGCGGTCGGTGCGGCATTTGAATGTCAGCGTCTGGGGCTGAAAGTTCCTGA
CGATATGGCGATTGCCGGTTTCCACGGTCATGACATTGGTCAGGTGATGGAGCCACGGCTGGCGAGCGTGCTGACGCCGC
GTGAGCGGATGGGCAGCATTGGCGCTGAGCGCCTGCTGGCGCGTATTCGTGGCGAATCTGTGACACCGAAAATGTTAGAT
TTAGGTTTCACCTTGTCACCGGGCGGATCTATTTAA

Upstream 100 bases:

>100_bases
CAGTATAAATTTATCTCACAACCTTCCTGAGGAATCGGGAAGGTTCTGATGCGTCCGTGGTAAACTAAAGGAATCTATCC
CTTTTGTACCTTTCAGGACG

Downstream 100 bases:

>100_bases
GCTCACAAATTTGAAGTAGCTCACACTTATACACTTAAGGCACGGATGGATATTGCTTCTGTTTTGGTCCGGCTAGACAA
TGTTACCGATAACAGTTACC

Product: DNA-binding transcriptional repressor

Products: NA

Alternate protein names: Gluconate utilization system GNT-I transcriptional repressor

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI

Sequences:

>Translated_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI
>Mature_331_residues
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAE
VLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAV
GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLD
LGFTLSPGGSI

Specific function: Negative regulator for the gluconate utilization system GNT-I, the gntUKR operon

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI48994940, Length=331, Percent_Identity=100, Blast_Score=679, Evalue=0.0,
Organism=Escherichia coli, GI1790715, Length=332, Percent_Identity=46.0843373493976, Blast_Score=304, Evalue=7e-84,
Organism=Escherichia coli, GI1790369, Length=323, Percent_Identity=29.4117647058824, Blast_Score=138, Evalue=6e-34,
Organism=Escherichia coli, GI1790194, Length=328, Percent_Identity=28.6585365853659, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787948, Length=319, Percent_Identity=27.5862068965517, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787906, Length=330, Percent_Identity=27.8787878787879, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788474, Length=307, Percent_Identity=25.7328990228013, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1787580, Length=317, Percent_Identity=25.5520504731861, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1789068, Length=317, Percent_Identity=25.2365930599369, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1786540, Length=325, Percent_Identity=26.1538461538462, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1789202, Length=293, Percent_Identity=22.8668941979522, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=23.2415902140673, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GNTR_ECOL6 (P0ACP6)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_756091.1
- ProteinModelPortal:   P0ACP6
- SMR:   P0ACP6
- EnsemblBacteria:   EBESCT00000040774
- GeneID:   1036975
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c4227
- GeneTree:   EBGT00050000009236
- HOGENOM:   HBG753640
- OMA:   EVVTPQM
- ProtClustDB:   PRK14987
- GO:   GO:0005622
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761
- SMART:   SM00354

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1; SSF47413 Lambda_like_DNA

EC number: NA

Molecular weight: Translated: 36423; Mature: 36423

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00356 HTH_LACI_1; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAVGFDNFEAARQMTTAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
HHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGESVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC
>Mature Secondary Structure
MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAALDELGYIPNRAPDILSN
CCCCCCHHHHHHHHHCHHHHHHHHHHCCHHHEEHHHHHHHHHHHHHHCCCCCCCHHHHHH
ATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYGYKPEMEQERLESMLSWNI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
DGLILTERTHTPRTLKMIEVAGIPVVELMDSKSPCLDIAVGFDNFEAARQMTTAIIARGH
CEEEEECCCCCCCCEEEEEECCCCHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCC
RHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVEQSSSYSSGIELIRQARREYPQL
HHHHHHHCCCCHHHHHHHCCHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
DGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHGHDIGQVMEPRLASVLTPRERMGSI
CCEEECCCCCEECCHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHHHHHCCCHHHHHCC
GAERLLARIRGESVTPKMLDLGFTLSPGGSI
CHHHHHHHHCCCCCCCHHHHCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157