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The map label for this gene is tusB [H]

Identifier: 218550604

GI number: 218550604

Start: 3375663

End: 3375950

Strand: Reverse

Name: tusB [H]

Synonym: EFER_3313

Alternate gene names: 218550604

Gene position: 3375950-3375663 (Counterclockwise)

Preceding gene: 218550605

Following gene: 218550603

Centisome position: 73.57

GC content: 50.69

Gene sequence:

>288_bases
ATGCTGCACACATTACATCGCTCACCATGGCTGACGGATTTTGCTGCGCTGCTGCGTCTGCTCAGTGAGGGCGACGAACT
GCTGTTATTGCAAGATGGCGTAACTGCCGCAGTTGACGGTAACCGCTACCTTGAAAGCCTGCGTAATGCCCCCATTAAGG
TCTATGCCCTTAACGAAGACCTTATTGCCCGCGGTTTGACTGGTCAAATTTCGAACGACATCATCCCTATTGACTATACT
GATTTCGTCAGACTTACGGTTAAGCATACCAGCCAGTTGGCCTGGTGA

Upstream 100 bases:

>100_bases
CCGCAGACATCCTTTGTTGTCGAAGCCACGCCGCTCGAAGCAGATGCCTTACGCCGCGAACTCGCCAACTACGATGTTAT
TTTGAGGTTTTGAGGCGTTT

Downstream 100 bases:

>100_bases
TGGCGGGATCGTTGTATATTTCTTGACACCTTTTCGGCATCGCCCTAAAATTCGGCGTCCTCATATTGTGTGAGGACGTT
TTATTACGTGTTTACGAAGC

Product: sulfur transfer complex subunit TusB

Products: NA

Alternate protein names: tRNA 2-thiouridine synthesizing protein B [H]

Number of amino acids: Translated: 95; Mature: 95

Protein sequence:

>95_residues
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYT
DFVRLTVKHTSQLAW

Sequences:

>Translated_95_residues
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYT
DFVRLTVKHTSQLAW
>Mature_95_residues
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYT
DFVRLTVKHTSQLAW

Specific function: Part of a sulfur-relay system required for 2-thiolation of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at tRNA wobble positions [H]

COG id: COG2168

COG function: function code P; Uncharacterized conserved protein involved in oxidation of intracellular sulfur

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dsrH/tusB family [H]

Homologues:

Organism=Escherichia coli, GI1789741, Length=95, Percent_Identity=96.8421052631579, Blast_Score=186, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007215 [H]

Pfam domain/function: PF04077 DsrH [H]

EC number: NA

Molecular weight: Translated: 10688; Mature: 10688

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNED
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHCCCCEEEEEECHH
LIARGLTGQISNDIIPIDYTDFVRLTVKHTSQLAW
HHHCCCCCCCCCCEECCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYLESLRNAPIKVYALNED
CCCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHCCCCEEEEEECHH
LIARGLTGQISNDIIPIDYTDFVRLTVKHTSQLAW
HHHCCCCCCCCCCEECCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA