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The map label for this gene is insF [H]

Identifier: 218550106

GI number: 218550106

Start: 2860840

End: 2861706

Strand: Direct

Name: insF [H]

Synonym: EFER_2792

Alternate gene names: 218550106

Gene position: 2860840-2861706 (Clockwise)

Preceding gene: 218550105

Following gene: 218550107

Centisome position: 62.35

GC content: 53.98

Gene sequence:

>867_bases
ATGAAGTATGTCTTTATTGAAAAACATCAGGCGGAGTTCAGCATCAAAGCAATGTGCCGTGTGCTCCGGGTGGCCCGCAG
CGGCTGGTATACATGGTGTCAGCGGCGTACAAGGATAAGCAAGCGTCAGCAGTTCCGTCAGCACTGCGACAGCGTTGTCC
GTGAGGCTTTTACCCGGTCAAAACAGCGTTACGGTGCACCACGCCTGACGGATGAACTGTGTGCTCAGGGTTACCACTTT
AACGTAAAAACCGTGGCGGCAAGCCTGCGCCGTCAGGGGCTGAGGGCAAAAGCCTCCCGGAAGTTCAGCCCGGTCAGTTA
CCGTGCACACAACCTGCCTGTGTCAGAAAATCTGCTGGAGCAGGATTTTTACGCCAGTGGCCCTAACCAGAAGTGGGCGG
GAGACATCACTTACTTACGTACAGATGAAGGCTGGCTGTATCTGGCAGTGGTCATTGACCTGTGGTCACGTGCCGTTATT
GGCTGGTCAATGTCGCCACGCATGACGGCGCAACTGGCCTGCGATGCCCTGCAGATGGCGCTGTGGCGGCGTAAGAGACC
CCGGAACGTTATCGTTCACACGGACCGCGGAAGCCAGTACTGTTCAGCAGATTATCAGGCGCTGCTGAAGCGGCATAATC
TGCGTGGCAGTATGAGCGCAAAGGGATGCTGCTACGATAATGCCTGCGTGGAAAGCTTCTTTCATTCGCTGAAAGTGGAA
TCTCTCCACGGAGAACACTTTATCAGCCGGGAAATAATGCGGGCAACAGTGTTTAATTATATCGAATGTGATTACAATCG
CTGGCGACGGCACAGTTGGTGTGGTGGTCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAG

Upstream 100 bases:

>100_bases
TGTCCACCGAGATTGCACGTCTCAAACGCCAGCTGGCAGAACGGGATGAAGAGCTGGCTATCCTCCAAAAGGCCGCGACA
TACTTTGCGAAGCGCCTGAA

Downstream 100 bases:

>100_bases
AGCTGTGTCCATATTACGAGGGTAGGATCAACGTTCAGCTATCTACCGTCATCCCGATGACTGGGATATATATCGACTTG
CAGAGGAGCTCATGCGTGAT

Product: IS3 element protein InsF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISKRQQFRQHCDSVVREAFTRSKQRYGAPRLTDELCAQGYHF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHNLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGSQYCSADYQALLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
SLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA

Sequences:

>Translated_288_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISKRQQFRQHCDSVVREAFTRSKQRYGAPRLTDELCAQGYHF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHNLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGSQYCSADYQALLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
SLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA
>Mature_288_residues
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISKRQQFRQHCDSVVREAFTRSKQRYGAPRLTDELCAQGYHF
NVKTVAASLRRQGLRAKASRKFSPVSYRAHNLPVSENLLEQDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVI
GWSMSPRMTAQLACDALQMALWRRKRPRNVIVHTDRGSQYCSADYQALLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
SLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA

Specific function: Involved in the transposition of the insertion sequence IS3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=242, Percent_Identity=33.4710743801653, Blast_Score=147, Evalue=9e-36,
Organism=Escherichia coli, GI1788405, Length=288, Percent_Identity=96.1805555555556, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1787263, Length=288, Percent_Identity=96.1805555555556, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1786753, Length=288, Percent_Identity=96.1805555555556, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1786570, Length=288, Percent_Identity=96.1805555555556, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1786490, Length=288, Percent_Identity=96.1805555555556, Blast_Score=578, Evalue=1e-166,
Organism=Escherichia coli, GI1789981, Length=273, Percent_Identity=31.5018315018315, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 33712; Mature: 33712

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISKRQQFRQHCDSVVREAFTRS
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELCAQGYHFNVKTVAASLRRQGLRAKASRKFSPVSYRAHNLPVSENLLE
HHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCEECCCCCCHHHHHH
QDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMA
HHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LWRRKRPRNVIVHTDRGSQYCSADYQALLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA
HHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MKYVFIEKHQAEFSIKAMCRVLRVARSGWYTWCQRRTRISKRQQFRQHCDSVVREAFTRS
CCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KQRYGAPRLTDELCAQGYHFNVKTVAASLRRQGLRAKASRKFSPVSYRAHNLPVSENLLE
HHHCCCCCCCHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCCCCCCEECCCCCCHHHHHH
QDFYASGPNQKWAGDITYLRTDEGWLYLAVVIDLWSRAVIGWSMSPRMTAQLACDALQMA
HHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LWRRKRPRNVIVHTDRGSQYCSADYQALLKRHNLRGSMSAKGCCYDNACVESFFHSLKVE
HHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHH
SLHGEHFISREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENQNLA
HHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987844; 9278503 [H]