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The map label for this gene is sbcD [H]

Identifier: 218549943

GI number: 218549943

Start: 2698251

End: 2699495

Strand: Direct

Name: sbcD [H]

Synonym: EFER_2628

Alternate gene names: 218549943

Gene position: 2698251-2699495 (Clockwise)

Preceding gene: 218549940

Following gene: 218549944

Centisome position: 58.8

GC content: 51.65

Gene sequence:

>1245_bases
ATGGGTATAATCGCCTTCACGTTTTTTCGCCAGGAAACTGTTATGCGCATCCTCCACACTTCAGACTGGCATCTGGGCCA
GAATTTTTACAGCAAGAGCCGCGCCGCGGAGCATCAGGCATTTCTCGACTGGCTGCTCACAACGGCACAAACTCATCAGG
TGGATGCGATAATTGTTGCCGGAGATATTTTCGATACCGGATCACCACCAAGTTATGCCCGCGAACTGTACAACCGCTTC
GTGGTCAATTTACAGCAAACGGGGTGTCATCTGGTGGTACTGGCAGGAAATCACGACTCGGTCGCCACACTGAATGAATC
ACGAGATATTCTCGCCTTTCTTAATACGACCGTTGTCGCCAGTGCCAACCTACGCCCACAGCTGCTGAAACGGCGTGACG
GCACTCCGGGTGCTGTTCTGTGCCCCATTCCGTTTTTACGTCCACGCGACATCGTGACCAGCCAGGCAGGGCTGTCAGGC
AGTGAAAAACAGCAACATCTGCTGGCCACAATCACGGACTACTACCAACAACAATATACGGAAGCCTGCAACCTGCGCGG
TGATCAGCAACTGCCGATTATTGCTACCGGACACCTTACCACCGTGGGTGCCAGTAAAAGTGACGCTGTACGTGACATTT
ATATCGGCACGCTGGATGCATTTCCGGCACAAAACTTCCCACCTGTAGATTACATCGCCCTTGGGCATATTCACCGGGCG
CAAATTGTTGGCGGACAGGAACATATTCGCTATTGCGGTTCCCCCATCCCGCTGAGTTTTGATGAGTGTGGCAAAACTAA
AAGCGTACATCTGGTGAGCTTTGCAGAAGGCAAATTACACAGTGTCGAAGCGCTGCCCGTGCCAATGACTCAGCCACTGG
CCGTATTGAAAGGTGATCTGGCTGCAATTACTGAACAGCTTGAACAATGGCGTGATACCGAACTGCAGACGCCAGTATGG
CTGGATATCGAAATCACGACTGATGACTATCTGCATGATATGCAGAGGAAAATCCAGACTTTAACCGAGTCACTGCCGGT
AGAAGTGTTACTGGTGCGTCGCAGTCGCGAGCAGCGTGAACGGATTATAGCGAGCGAGAAACGCGAAACACTCAATGAAT
TAAGCGTCGAGGAAGTTTTTAATCGTCGCCTGGCACTGGAAGAACTGGACGAGTCGCAACATGCGCGTATGCAACATCTT
TTCGCCACGACGTTGCAGACCCTTGCAGGAGATCACGAAGCATGA

Upstream 100 bases:

>100_bases
ATTATGCGTCAGTTTTATGACAGATTTATGAAAAGATCGTCGATGTTTCGTACAGGTTATTGATTTGTAGAGGTCATAAA
ACAGAAATTAATGCCTGTCT

Downstream 100 bases:

>100_bases
AAATCCTCAGCCTGCGCCTGAAAAACCTGAACTCTCTGAAAGGCGAATGGAAGATTGATTTCACCACAGAACCGTTTGCC
AGCAACGGACTGTTTGCCAT

Product: exonuclease subunit SbcD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MGIIAFTFFRQETVMRILHTSDWHLGQNFYSKSRAAEHQAFLDWLLTTAQTHQVDAIIVAGDIFDTGSPPSYARELYNRF
VVNLQQTGCHLVVLAGNHDSVATLNESRDILAFLNTTVVASANLRPQLLKRRDGTPGAVLCPIPFLRPRDIVTSQAGLSG
SEKQQHLLATITDYYQQQYTEACNLRGDQQLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPVDYIALGHIHRA
QIVGGQEHIRYCGSPIPLSFDECGKTKSVHLVSFAEGKLHSVEALPVPMTQPLAVLKGDLAAITEQLEQWRDTELQTPVW
LDIEITTDDYLHDMQRKIQTLTESLPVEVLLVRRSREQRERIIASEKRETLNELSVEEVFNRRLALEELDESQHARMQHL
FATTLQTLAGDHEA

Sequences:

>Translated_414_residues
MGIIAFTFFRQETVMRILHTSDWHLGQNFYSKSRAAEHQAFLDWLLTTAQTHQVDAIIVAGDIFDTGSPPSYARELYNRF
VVNLQQTGCHLVVLAGNHDSVATLNESRDILAFLNTTVVASANLRPQLLKRRDGTPGAVLCPIPFLRPRDIVTSQAGLSG
SEKQQHLLATITDYYQQQYTEACNLRGDQQLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPVDYIALGHIHRA
QIVGGQEHIRYCGSPIPLSFDECGKTKSVHLVSFAEGKLHSVEALPVPMTQPLAVLKGDLAAITEQLEQWRDTELQTPVW
LDIEITTDDYLHDMQRKIQTLTESLPVEVLLVRRSREQRERIIASEKRETLNELSVEEVFNRRLALEELDESQHARMQHL
FATTLQTLAGDHEA
>Mature_413_residues
GIIAFTFFRQETVMRILHTSDWHLGQNFYSKSRAAEHQAFLDWLLTTAQTHQVDAIIVAGDIFDTGSPPSYARELYNRFV
VNLQQTGCHLVVLAGNHDSVATLNESRDILAFLNTTVVASANLRPQLLKRRDGTPGAVLCPIPFLRPRDIVTSQAGLSGS
EKQQHLLATITDYYQQQYTEACNLRGDQQLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPVDYIALGHIHRAQ
IVGGQEHIRYCGSPIPLSFDECGKTKSVHLVSFAEGKLHSVEALPVPMTQPLAVLKGDLAAITEQLEQWRDTELQTPVWL
DIEITTDDYLHDMQRKIQTLTESLPVEVLLVRRSREQRERIIASEKRETLNELSVEEVFNRRLALEELDESQHARMQHLF
ATTLQTLAGDHEA

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=400, Percent_Identity=86.5, Blast_Score=728, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46435; Mature: 46304

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIIAFTFFRQETVMRILHTSDWHLGQNFYSKSRAAEHQAFLDWLLTTAQTHQVDAIIVA
CCEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GDIFDTGSPPSYARELYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDILAFLNTTVVA
CCCCCCCCCHHHHHHHHHHHHEEEECCCEEEEEEECCCCCEEEECCCCCHHHHHHHHEEE
SANLRPQLLKRRDGTPGAVLCPIPFLRPRDIVTSQAGLSGSEKQQHLLATITDYYQQQYT
ECCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EACNLRGDQQLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPVDYIALGHIHRA
HHHCCCCCCCCCEEEECCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCEEECCCCCHH
QIVGGQEHIRYCGSPIPLSFDECGKTKSVHLVSFAEGKLHSVEALPVPMTQPLAVLKGDL
HCCCCHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHHHH
AAITEQLEQWRDTELQTPVWLDIEITTDDYLHDMQRKIQTLTESLPVEVLLVRRSREQRE
HHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
RIIASEKRETLNELSVEEVFNRRLALEELDESQHARMQHLFATTLQTLAGDHEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
GIIAFTFFRQETVMRILHTSDWHLGQNFYSKSRAAEHQAFLDWLLTTAQTHQVDAIIVA
CEEEEEHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEE
GDIFDTGSPPSYARELYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDILAFLNTTVVA
CCCCCCCCCHHHHHHHHHHHHEEEECCCEEEEEEECCCCCEEEECCCCCHHHHHHHHEEE
SANLRPQLLKRRDGTPGAVLCPIPFLRPRDIVTSQAGLSGSEKQQHLLATITDYYQQQYT
ECCCCHHHHHHCCCCCCEEECCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
EACNLRGDQQLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPVDYIALGHIHRA
HHHCCCCCCCCCEEEECCEEEECCCCCCCHHEEEEEECCCCCCCCCCCCCEEECCCCCHH
QIVGGQEHIRYCGSPIPLSFDECGKTKSVHLVSFAEGKLHSVEALPVPMTQPLAVLKGDL
HCCCCHHHHHHCCCCCCCCHHHHCCCCCEEEEEECCCCCCCEEECCCCCCCCHHHHHHHH
AAITEQLEQWRDTELQTPVWLDIEITTDDYLHDMQRKIQTLTESLPVEVLLVRRSREQRE
HHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHH
RIIASEKRETLNELSVEEVFNRRLALEELDESQHARMQHLFATTLQTLAGDHEA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]