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The map label for this gene is kstD [H]

Identifier: 218549910

GI number: 218549910

Start: 2664604

End: 2666307

Strand: Direct

Name: kstD [H]

Synonym: EFER_2595

Alternate gene names: 218549910

Gene position: 2664604-2666307 (Clockwise)

Preceding gene: 218549909

Following gene: 218549911

Centisome position: 58.07

GC content: 52.41

Gene sequence:

>1704_bases
ATGAGCGGGCAAATCAAGGTTGATGTTCTGGTGGTCGGTTCAGGTGCGGCTGGTTTATCAGCGGCAGTAACTGCCGCCCA
TTTTGGCGCCAAAGTCCTGGTGGCGGAAAAAGAGTCTGTTATCGGTGGCACCAGTGCCTGGTCGGGTGGTTGGTTGTGGA
TCCCGCGTAATCCACTCGCCCGGGAAGAAGGTTTTCAGGAAGATGCCAGTGCACCGCTCAATTATCTCAGCCACGAAATG
GCAGGTCAGGCAGCTGATGCCAGGCTGAAAGCATTTTTACACTATGGCCCGGAAATGATTGAGTTTTTTCGTCAGAACAC
TGCTGTCCAGTTTTTGTCCGGTAGCAGAATGCCTGATTTTCATAACTCGCCAGGATTTGCTCATGGAGGGCGCTCAGTTA
CGGCACAACCTTTCGATGGAAAACAGTTAGGAGAATGGCTATACCGGTTGCGTCCACCACTGGAAGTTATTAGCCTGGCA
GGCATGGGGATTGCTGGCGGAAGCGATATGGCGCATTTTTTTAATGCTACTCGTTCGCCGCGCTCTGCCTTTTATGCTAC
ACGTCGCCTGTTGCGTCATGGATGGCAGCGTTTGCGTTATGGTCGTGGACAGCATCTGGTGAATGGCAATGCTCTGGTGG
CACGCTTGCTGCGTTCGGCACTGGATCTGGGCGTAAAGTTTCAGCTGAATGCCCCGGTTGGACAACTCATTCACTCACTG
AAAGGCATTGAAGGTGCAACGCTGCGAACAGACAGCGGCAATCTGACTGTTAAAGCTAACGCGGTGATTCTTGCCTGCGG
TGGTTTTCCCCATGATCGCCAGCGTCTGGCAAAACATGTACCACACGCCACTGAAGGGTACGGGCATTTTTCCGCCGCTC
CGCCAGGTAATCAGGGTGATGGTATCCGGCTGGGAGAATCCGTTGGTGGACAATTTGATCACACTCTCAAGCATCCAATG
GCGTGGGCCCCGGTGTCACGTGTCACTCTCGATAGTGGATTACAGCTGGTTTTCCCGCATCTGGTTGAACGCGCCAAACC
TGGTTTTATCGCTGTTCTCCCTAATGGTAAGCGCTTTGTTAATGAAGCTGATTCATACCATGATTTTATCGCTGCTTTAC
TCGCCGCCACACCTGAAGGCGAAACACCACACGCCTGGCTACTGGCAGACGGCCCCTCCATGCGCCGATACGGCCTTGGT
CACGCACGCCCGTCACCTTTCAATGCCCAGGCATGGGTCCGTTCAGGGTATCTGCAAACCGCCAACTCACTGACAGCCTT
AGCAGAAAAGTGCGCGATTGATGGCCCCCAATTGATGGCAACGGTGTCACAGTTTAATGCTGCAGCAGAAAGTGGGGTTG
ATAACGAATTTCAACGTGGAGCATCCGCATACAACCTGGCACAAGGCGACACGCAACATCCTTTGCATCCTACGCTTGGT
CCATTATGCCATCCTCCATTTTACGCTGTACGCATTCTTCCGGGATCACTCGGTTCATTTAGCGGATTGATTACTGATGA
ACACGCCCGTGTTCTGAATGCCCAACAACAACCAATAAACGGCCTGTATGCCATTGGTAATGATATGTCGAGTGTGATGC
GTGGGTTTTATCCCAGCGGTGGTATTACGCTCGGCCCGGCCATGACCTTCGGTTATATGGTTGGGAAACAACTGTCAGCA
GAAAGAGAGAAAAGCATCGATTAA

Upstream 100 bases:

>100_bases
ACCTTTTCGCGCAAGAATCGCGAACTGGCCGCTGTTGAACGTCTTGAGTCGGTGTTGATGCGTCCGACAGATTTCTCCCC
ACTGCAATAAGGAGCAGGCC

Downstream 100 bases:

>100_bases
AAGTTAATTTCCCAGGGAAGGCAGCACACATAACAACAATTCACCTGCAACTGTACTGGAGTCATTTATGAACACACGTA
CCCTGTCTATTGCCGCCTTA

Product: hypothetical protein

Products: NA

Alternate protein names: 3-keto-Delta(4)-steroid Delta(1)-dehydrogenase; KSDD; 3-oxo-Delta(4)-steroid 1-dehydrogenase; KSTD [H]

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MSGQIKVDVLVVGSGAAGLSAAVTAAHFGAKVLVAEKESVIGGTSAWSGGWLWIPRNPLAREEGFQEDASAPLNYLSHEM
AGQAADARLKAFLHYGPEMIEFFRQNTAVQFLSGSRMPDFHNSPGFAHGGRSVTAQPFDGKQLGEWLYRLRPPLEVISLA
GMGIAGGSDMAHFFNATRSPRSAFYATRRLLRHGWQRLRYGRGQHLVNGNALVARLLRSALDLGVKFQLNAPVGQLIHSL
KGIEGATLRTDSGNLTVKANAVILACGGFPHDRQRLAKHVPHATEGYGHFSAAPPGNQGDGIRLGESVGGQFDHTLKHPM
AWAPVSRVTLDSGLQLVFPHLVERAKPGFIAVLPNGKRFVNEADSYHDFIAALLAATPEGETPHAWLLADGPSMRRYGLG
HARPSPFNAQAWVRSGYLQTANSLTALAEKCAIDGPQLMATVSQFNAAAESGVDNEFQRGASAYNLAQGDTQHPLHPTLG
PLCHPPFYAVRILPGSLGSFSGLITDEHARVLNAQQQPINGLYAIGNDMSSVMRGFYPSGGITLGPAMTFGYMVGKQLSA
EREKSID

Sequences:

>Translated_567_residues
MSGQIKVDVLVVGSGAAGLSAAVTAAHFGAKVLVAEKESVIGGTSAWSGGWLWIPRNPLAREEGFQEDASAPLNYLSHEM
AGQAADARLKAFLHYGPEMIEFFRQNTAVQFLSGSRMPDFHNSPGFAHGGRSVTAQPFDGKQLGEWLYRLRPPLEVISLA
GMGIAGGSDMAHFFNATRSPRSAFYATRRLLRHGWQRLRYGRGQHLVNGNALVARLLRSALDLGVKFQLNAPVGQLIHSL
KGIEGATLRTDSGNLTVKANAVILACGGFPHDRQRLAKHVPHATEGYGHFSAAPPGNQGDGIRLGESVGGQFDHTLKHPM
AWAPVSRVTLDSGLQLVFPHLVERAKPGFIAVLPNGKRFVNEADSYHDFIAALLAATPEGETPHAWLLADGPSMRRYGLG
HARPSPFNAQAWVRSGYLQTANSLTALAEKCAIDGPQLMATVSQFNAAAESGVDNEFQRGASAYNLAQGDTQHPLHPTLG
PLCHPPFYAVRILPGSLGSFSGLITDEHARVLNAQQQPINGLYAIGNDMSSVMRGFYPSGGITLGPAMTFGYMVGKQLSA
EREKSID
>Mature_566_residues
SGQIKVDVLVVGSGAAGLSAAVTAAHFGAKVLVAEKESVIGGTSAWSGGWLWIPRNPLAREEGFQEDASAPLNYLSHEMA
GQAADARLKAFLHYGPEMIEFFRQNTAVQFLSGSRMPDFHNSPGFAHGGRSVTAQPFDGKQLGEWLYRLRPPLEVISLAG
MGIAGGSDMAHFFNATRSPRSAFYATRRLLRHGWQRLRYGRGQHLVNGNALVARLLRSALDLGVKFQLNAPVGQLIHSLK
GIEGATLRTDSGNLTVKANAVILACGGFPHDRQRLAKHVPHATEGYGHFSAAPPGNQGDGIRLGESVGGQFDHTLKHPMA
WAPVSRVTLDSGLQLVFPHLVERAKPGFIAVLPNGKRFVNEADSYHDFIAALLAATPEGETPHAWLLADGPSMRRYGLGH
ARPSPFNAQAWVRSGYLQTANSLTALAEKCAIDGPQLMATVSQFNAAAESGVDNEFQRGASAYNLAQGDTQHPLHPTLGP
LCHPPFYAVRILPGSLGSFSGLITDEHARVLNAQQQPINGLYAIGNDMSSVMRGFYPSGGITLGPAMTFGYMVGKQLSAE
REKSID

Specific function: Catalyzes the elimination of the C-1 and C-2 hydrogen atoms of the A-ring from the polycyclic ring structure of 3- ketosteroids. Is also involved in the formation of 3-keto-1,4- diene-steroid from 3-keto-4-ene-steroid [H]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. 3-oxosteroid dehydrogenase subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.3.99.4 [H]

Molecular weight: Translated: 60801; Mature: 60669

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGQIKVDVLVVGSGAAGLSAAVTAAHFGAKVLVAEKESVIGGTSAWSGGWLWIPRNPLA
CCCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCEEEECCCCCC
REEGFQEDASAPLNYLSHEMAGQAADARLKAFLHYGPEMIEFFRQNTAVQFLSGSRMPDF
HHCCCCHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCC
HNSPGFAHGGRSVTAQPFDGKQLGEWLYRLRPPLEVISLAGMGIAGGSDMAHFFNATRSP
CCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCH
RSAFYATRRLLRHGWQRLRYGRGQHLVNGNALVARLLRSALDLGVKFQLNAPVGQLIHSL
HHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
KGIEGATLRTDSGNLTVKANAVILACGGFPHDRQRLAKHVPHATEGYGHFSAAPPGNQGD
CCCCCCEEEECCCCEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GIRLGESVGGQFDHTLKHPMAWAPVSRVTLDSGLQLVFPHLVERAKPGFIAVLPNGKRFV
CEEECHHCCCCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHH
NEADSYHDFIAALLAATPEGETPHAWLLADGPSMRRYGLGHARPSPFNAQAWVRSGYLQT
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHCHHHH
ANSLTALAEKCAIDGPQLMATVSQFNAAAESGVDNEFQRGASAYNLAQGDTQHPLHPTLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCCCCCCCCC
PLCHPPFYAVRILPGSLGSFSGLITDEHARVLNAQQQPINGLYAIGNDMSSVMRGFYPSG
CCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCC
GITLGPAMTFGYMVGKQLSAEREKSID
CEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGQIKVDVLVVGSGAAGLSAAVTAAHFGAKVLVAEKESVIGGTSAWSGGWLWIPRNPLA
CCCEEEEEEEEECCCCCHHHHHHHHHCCCEEEEECCCHHCCCCCCCCCCEEEECCCCCC
REEGFQEDASAPLNYLSHEMAGQAADARLKAFLHYGPEMIEFFRQNTAVQFLSGSRMPDF
HHCCCCHHHCCHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHCCCEEECCCCCCCCC
HNSPGFAHGGRSVTAQPFDGKQLGEWLYRLRPPLEVISLAGMGIAGGSDMAHFFNATRSP
CCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCCCHHHHHHHCCCCH
RSAFYATRRLLRHGWQRLRYGRGQHLVNGNALVARLLRSALDLGVKFQLNAPVGQLIHSL
HHHHHHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCEEEEECCCHHHHHHHH
KGIEGATLRTDSGNLTVKANAVILACGGFPHDRQRLAKHVPHATEGYGHFSAAPPGNQGD
CCCCCCEEEECCCCEEEEEEEEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
GIRLGESVGGQFDHTLKHPMAWAPVSRVTLDSGLQLVFPHLVERAKPGFIAVLPNGKRFV
CEEECHHCCCCCHHHHHCCCCCCCHHHHHHHCCHHHHHHHHHHHCCCCEEEECCCCHHHH
NEADSYHDFIAALLAATPEGETPHAWLLADGPSMRRYGLGHARPSPFNAQAWVRSGYLQT
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHCHHHH
ANSLTALAEKCAIDGPQLMATVSQFNAAAESGVDNEFQRGASAYNLAQGDTQHPLHPTLG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCCHHCCCCCCCCCCCCCCCC
PLCHPPFYAVRILPGSLGSFSGLITDEHARVLNAQQQPINGLYAIGNDMSSVMRGFYPSG
CCCCCCCEEEEECCCCCCCCCCCEEHHHHHHHCCCCCCCCCEEEECCCHHHHHHCCCCCC
GITLGPAMTFGYMVGKQLSAEREKSID
CEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036 [H]