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The map label for this gene is acrA [H]

Identifier: 218549869

GI number: 218549869

Start: 2618859

End: 2620052

Strand: Direct

Name: acrA [H]

Synonym: EFER_2554

Alternate gene names: 218549869

Gene position: 2618859-2620052 (Clockwise)

Preceding gene: 218549866

Following gene: 218549870

Centisome position: 57.07

GC content: 52.93

Gene sequence:

>1194_bases
ATGAACAAAAACAGAGGATTTACGCCTCTGGCGGCCGTTCTGTTGCTTTCAGGCAGCTTAGCGCTAACAGGATGCGACGA
CAAACAGGCCCAACAAGGGGGCCAGCAGATGCCAGAAGTTGGGGTTGTGACGCTCAAAACTGAACCTCTACAAATCACTA
CTGAGCTTCCGGGGCGTACCAGCGCTTTCCGTATTGCGGAAGTCCGTCCTCAGGTTAGCGGTATTATCCTGAAGCGCAAT
TTTAAAGAAGGCAGTGATATCGAAGCTGGCGTCTCTCTGTATCAGATTGATCCTGCAACTTATCAGGCTGCCTATGACAG
CGCGAAAGGCGATCTGGCGAAAGCTCAGGCTGCGGCTAATATCGCGCAACTGACAGTTAATCGTTATCAGAAACTGCTCG
GTACGCAGTACATCAGTAAGCAGGAGTACGATCAGGCACTGGCTGACGCGCAACAGGCTAACGCAGCGGTGACCGCGGCG
AAAGCGGCTGTTGAAACCGCACGGATTAACCTGGCATACACCAAAGTCACCTCTCCAATTAATGGTCGCATTGGTAAGTC
GAATGTGACTGAAGGTGCCCTGGTGCAAAACGGTCAGGCGACTGCGCTGGCAACCGTACAGCAACTTGATCCGATCTATG
TTGATGTGACTCAGTCCAGCAACGACTTTCTGCGCCTGAAACAGGAACTGGCGAATGGCACGCTGAAACAAGAGAACGGC
AAAGCCAAAGTGTCGCTGATCACCAGTGACGGCATTAAGTTCCCACAGGACGGTACGCTGGAGTTCTCTGACGTTACTGT
CGATCAGACCACCGGGTCTATCACCCTACGCGCTATCTTCCCGAACCCGGATCATACTCTGCTGCCGGGTATGTTCGTGC
GTGCACGTCTGGAAGAAGGGCTTAATCCAAACGCCATTTTAGTCCCGCAACAGGGTGTAACCCGTATACCGCGTGGCGAT
GCTACCGTACTGGTGGTTGGCGCGGATGACAAAGTGGAAACCCGTCCGATCGTTGCAAGCCAGGCTATTGGCGATAAGTG
GCTGGTGACAGAAGGTCTGAAAGCAGGCGATCGCGTAGTAATAAGTGGGCTGCAGAAAGTGCGTCCTGGTGTCCAGGTAA
AAGCACAAGAAGTTACCGCTGATAATAACCAGCAAGCCGCAAGCGGTACTCAGCCTGAACAGTCCAAGTCTTAA

Upstream 100 bases:

>100_bases
ATGTATGTACCATATCACGACGATAATATAAACGCAGCAATGGGTTTAGGGGCTTTTTTGCCCATTGATCAATTTGAAAT
CGGACACTCGAGGTTTACAT

Downstream 100 bases:

>100_bases
CTTAAACAGGAGCCGTTAAGACATGCCTAATTTCTTTATCGATCGCCCGATTTTTGCGTGGGTGATCGCCATCATTATCA
TGTTGGCAGGGGGGCTGGCG

Product: multidrug efflux system

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MNKNRGFTPLAAVLLLSGSLALTGCDDKQAQQGGQQMPEVGVVTLKTEPLQITTELPGRTSAFRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPINGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRIPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGTQPEQSKS

Sequences:

>Translated_397_residues
MNKNRGFTPLAAVLLLSGSLALTGCDDKQAQQGGQQMPEVGVVTLKTEPLQITTELPGRTSAFRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPINGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRIPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGTQPEQSKS
>Mature_397_residues
MNKNRGFTPLAAVLLLSGSLALTGCDDKQAQQGGQQMPEVGVVTLKTEPLQITTELPGRTSAFRIAEVRPQVSGIILKRN
FKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
KAAVETARINLAYTKVTSPINGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRIPRGD
ATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGTQPEQSKS

Specific function: AcrAB is a drug efflux protein with a broad substrate specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786668, Length=397, Percent_Identity=97.7329974811083, Blast_Score=739, Evalue=0.0,
Organism=Escherichia coli, GI1789665, Length=375, Percent_Identity=68.5333333333333, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1789929, Length=382, Percent_Identity=54.9738219895288, Blast_Score=406, Evalue=1e-114,
Organism=Escherichia coli, GI87082036, Length=338, Percent_Identity=30.1775147928994, Blast_Score=141, Evalue=9e-35,
Organism=Escherichia coli, GI1789637, Length=150, Percent_Identity=30.6666666666667, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI87081951, Length=117, Percent_Identity=35.042735042735, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42246; Mature: 42246

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNRGFTPLAAVLLLSGSLALTGCDDKQAQQGGQQMPEVGVVTLKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCC
SAFRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAAN
CEEEEEECCCCCEEEEEEECCCCCCCCHHCEEEEEECCHHHHHHHHCCCCHHHHHHHHHH
IAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEECCCC
NGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
CCCCCCCCCCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
LNPNAILVPQQGVTRIPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVV
CCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEHHHHCCCCEEEECCCCCCCHHH
ISGLQKVRPGVQVKAQEVTADNNQQAASGTQPEQSKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKNRGFTPLAAVLLLSGSLALTGCDDKQAQQGGQQMPEVGVVTLKTEPLQITTELPGRT
CCCCCCCCHHHHHHHHCCCEEEECCCHHHHHHCCCCCCCCCEEEEECCCEEEEECCCCCC
SAFRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQAAYDSAKGDLAKAQAAAN
CEEEEEECCCCCEEEEEEECCCCCCCCHHCEEEEEECCHHHHHHHHCCCCHHHHHHHHHH
IAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAAKAAVETARINLAYTKVTSPI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEEEEEECCCC
NGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQSSNDFLRLKQELANGTLKQENG
CCCCCCCCCCCCCEEECCCCCEEHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCEECCCC
KAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITLRAIFPNPDHTLLPGMFVRARLEEG
CEEEEEEECCCCCCCCCCCEEEEEEEEECCCCEEEEEEECCCCCCCCCCHHHHHHHHHCC
LNPNAILVPQQGVTRIPRGDATVLVVGADDKVETRPIVASQAIGDKWLVTEGLKAGDRVV
CCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCEEHHHHCCCCEEEECCCCCCCHHH
ISGLQKVRPGVQVKAQEVTADNNQQAASGTQPEQSKS
HHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]