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The map label for this gene is lipB [H]

Identifier: 218549794

GI number: 218549794

Start: 2539278

End: 2539928

Strand: Direct

Name: lipB [H]

Synonym: EFER_2475

Alternate gene names: 218549794

Gene position: 2539278-2539928 (Clockwise)

Preceding gene: 218549793

Following gene: 218549795

Centisome position: 55.34

GC content: 49.77

Gene sequence:

>651_bases
TTGTATCAGGATAAAATTCTCGTCCGCCAGCTCGGCCTACAGCCCTATGAGCCGGTTTCCCAGGCAATGCATGAATTCAC
CGATACCCGCGATGAAAACACTCCTGACGAAATTTGGCTGGTTGAGCATTACCCTGTTTTTACTCAGGGTCAGGCCGGAA
AAGCAGAGCATGTGTTAATGCCGGGCGATATTCCAGTGATTCAAAGCGATCGGGGTGGCCAGGTAACCTACCATGGCCCT
GGACAGCAGGTGATGTATGTGCTGCTTAACCTGAAACGTCGCAAACTGGGCGTGCGTGAACTGGTGACTTTACTTGAGCA
AACTGTGGTTAATACTCTTGCAGAACTTGGAATTGACGCTCACCCACGTGCTGATGCTCCGGGCGTCTATGTAGGAGAAA
AGAAAATTTGTTCGTTGGGATTACGGATTCGCCGCGGCTGCTCATTTCACGGGTTAGCATTAAACGTCAATATGGACCTC
GAGCCTTTCCAGCGGATTAACCCCTGTGGCTATGCCGGCATGGAGATGGCAAAAGTTGCGCAATGGCAAAGTGAGGCGAC
CACCGATACGATTTCCCCACGCTTGTTGGCAAACATTTTAGCGTTGCTGAATAATCCACCTTACGAGTACGTTACTTTTT
CTCAACAGTAA

Upstream 100 bases:

>100_bases
TTCGCATCTCCGTTACCCGGCCCCTGGTCGGGTAACTCCCCTCTCCTCACTGTGATATACTTTTTCCCCTCTTTTACTCA
TTCTCCACGGAGATGCCGTT

Downstream 100 bases:

>100_bases
GCACTGCTGAAATGGCACAAAACAACAACTATTTTACATTTTAGCGACCAGTCAGGCTGCTTTAAAGGCAGTTTCAATGA
TATACTGCCAGTCGTTAATT

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MYQDKILVRQLGLQPYEPVSQAMHEFTDTRDENTPDEIWLVEHYPVFTQGQAGKAEHVLMPGDIPVIQSDRGGQVTYHGP
GQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIDAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
EPFQRINPCGYAGMEMAKVAQWQSEATTDTISPRLLANILALLNNPPYEYVTFSQQ

Sequences:

>Translated_216_residues
MYQDKILVRQLGLQPYEPVSQAMHEFTDTRDENTPDEIWLVEHYPVFTQGQAGKAEHVLMPGDIPVIQSDRGGQVTYHGP
GQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIDAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
EPFQRINPCGYAGMEMAKVAQWQSEATTDTISPRLLANILALLNNPPYEYVTFSQQ
>Mature_216_residues
MYQDKILVRQLGLQPYEPVSQAMHEFTDTRDENTPDEIWLVEHYPVFTQGQAGKAEHVLMPGDIPVIQSDRGGQVTYHGP
GQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIDAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
EPFQRINPCGYAGMEMAKVAQWQSEATTDTISPRLLANILALLNNPPYEYVTFSQQ

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Homo sapiens, GI221554520, Length=132, Percent_Identity=37.8787878787879, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI87081767, Length=212, Percent_Identity=91.0377358490566, Blast_Score=411, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6323268, Length=172, Percent_Identity=33.1395348837209, Blast_Score=89, Evalue=4e-19,
Organism=Drosophila melanogaster, GI21355483, Length=147, Percent_Identity=34.6938775510204, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 24232; Mature: 24232

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00092 N6_MTASE ; PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQDKILVRQLGLQPYEPVSQAMHEFTDTRDENTPDEIWLVEHYPVFTQGQAGKAEHVLM
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCCCEEEC
PGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIDA
CCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
HPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLEPFQRINPCGYAGMEMAKVA
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCCCHHHHHHHH
QWQSEATTDTISPRLLANILALLNNPPYEYVTFSQQ
HHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC
>Mature Secondary Structure
MYQDKILVRQLGLQPYEPVSQAMHEFTDTRDENTPDEIWLVEHYPVFTQGQAGKAEHVLM
CCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEECCCCCCCCEEEC
PGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELVTLLEQTVVNTLAELGIDA
CCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCC
HPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDLEPFQRINPCGYAGMEMAKVA
CCCCCCCCEEECCHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHCCCCCCCHHHHHHHH
QWQSEATTDTISPRLLANILALLNNPPYEYVTFSQQ
HHHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA