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The map label for this gene is gltJ

Identifier: 218549770

GI number: 218549770

Start: 2514261

End: 2515001

Strand: Direct

Name: gltJ

Synonym: EFER_2449

Alternate gene names: 218549770

Gene position: 2514261-2515001 (Clockwise)

Preceding gene: 218549769

Following gene: 218549771

Centisome position: 54.79

GC content: 51.15

Gene sequence:

>741_bases
ATGTCTATAGACTGGAACTGGGGTATTTTTTTACAACAAGCCCCGTTCGGCAACACCACCTATCTTGGTTGGATCTGGAG
TGGTTTTCAGGTGACGATTGCTTTGTCGATCTGCGCCTGGATTATCGCCTTCCTCGTCGGTTCATTTTTCGGCATTTTAC
GTACCGTTCCAAACCGTTTTCTCTCCGGTCTGGGTACGTTGTATGTCGAACTGTTCCGCAACGTGCCGTTGATTGTGCAA
TTCTTTACCTGGTATCTGGTTATCCCTGAACTGTTGCCGGAAAAAATCGGCATGTGGTTTAAGGCAGAACTAGACCCAAA
TATTCAGTTTTTCCTCTCCTCCATGCTCTGTCTGGGACTGTTTACTGCAGCTCGTGTTTGCGAACAAGTACGCGCCGCGA
TTCAGTCGTTGCCGCGTGGGCAAAAGAATGCCGCGCTGGCAATGGGTCTGACGCTACCGCAGGCTTACCGCTACGTACTG
TTGCCTAACGCTTATCGCGTTATCGTCCCGCCAATGACCTCTGAGATGATGAACCTGGTAAAAAACTCCGCCATCGCCTC
GACCATCGGTCTGGTGGATATGGCGGCGCAGGCCGGTAAATTGCTCGATTACTCGGCCCACGCCTGGGAGTCATTTACCG
CCATTACGCTGGCGTATGTGTTGATTAACGCTTTTATCATGCTGGTGATGACGCTGGTTGAACGTAAAGTTCGCCTGCCC
GGCAACATGGGGGGCAAATAA

Upstream 100 bases:

>100_bases
ATGGTCGTTCCCCATCGGGCCTGAAAACCGCAATACGCTGGGTAACAATCTTCGAGGGTAGCAGTTAACGCTGCTACCCT
CTTTTTTCTGGAGTAGATTT

Downstream 100 bases:

>100_bases
TGTACGAGTTTGACTGGAGTTCCATTGTTCCTTCCCTGCCATATCTGCTCGACGGGTTGGTGATCACCCTGAAAATCACC
ATCACGGCGGTGGTCATTGG

Product: glutamate and aspartate ABC transporter membrane protein

Products: ADP; phosphate; L-glutamate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQ
FFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
GNMGGK

Sequences:

>Translated_246_residues
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQ
FFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
GNMGGK
>Mature_245_residues
SIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQF
FTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLL
PNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLPG
NMGGK

Specific function: Part of the binding-protein-dependent transport system for aspartate/glutamate; probably responsible for the translocation of the substrate across the membrane

COG id: COG0765

COG function: function code E; ABC-type amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain

Homologues:

Organism=Escherichia coli, GI1786874, Length=246, Percent_Identity=100, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1788226, Length=198, Percent_Identity=32.8282828282828, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI48994930, Length=123, Percent_Identity=39.8373983739837, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1786873, Length=215, Percent_Identity=26.046511627907, Blast_Score=95, Evalue=3e-21,
Organism=Escherichia coli, GI1787030, Length=213, Percent_Identity=23.4741784037559, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788645, Length=217, Percent_Identity=27.6497695852535, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787086, Length=218, Percent_Identity=26.605504587156, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788646, Length=221, Percent_Identity=28.5067873303167, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082239, Length=131, Percent_Identity=28.2442748091603, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787087, Length=222, Percent_Identity=23.8738738738739, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLTJ_ECOL6 (P0AER4)

Other databases:

- EMBL:   AE014075
- RefSeq:   NP_752668.1
- ProteinModelPortal:   P0AER4
- EnsemblBacteria:   EBESCT00000044309
- GeneID:   1035918
- GenomeReviews:   AE014075_GR
- KEGG:   ecc:c0738
- GeneTree:   EBGT00050000008885
- HOGENOM:   HBG704821
- OMA:   FFIWYLV
- ProtClustDB:   CLSK879719
- InterPro:   IPR010065
- InterPro:   IPR000515
- TIGRFAMs:   TIGR01726

Pfam domain/function: PF00528 BPD_transp_1

EC number: NA

Molecular weight: Translated: 27503; Mature: 27372

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x2a4dee34)-; HASH(0x29d9c384)-; HASH(0x2825e9a8)-; HASH(0x2a7abff8)-; HASH(0x2a5ccf58)-;

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRF
CCCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEEECCCCCEEEECCHHHHHHHHH
KNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
HHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNMGGK
CCCCCH
>Mature Secondary Structure 
SIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAFLVGSFFGILRTVPNRF
CCCCCCCEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKAELDPNIQFFLSSMLCLGL
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHH
FTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVLLPNAYRVIVPPMTSEMMNLV
HHHHHHHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHEEECCCCCEEEECCHHHHHHHHH
KNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLAYVLINAFIMLVMTLVERKVRLP
HHHHHHHHHHHHHHHHHHCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GNMGGK
CCCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate [Periplasm]; H2O [C]

Specific reaction: ATP + L-glutamate [Periplasm] + H2O = ADP + phosphate + L-glutamate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157