Click here to switch to the map view.

The map label for this gene is PduD [H]

Identifier: 218549344

GI number: 218549344

Start: 2064227

End: 2064889

Strand: Direct

Name: PduD [H]

Synonym: EFER_2011

Alternate gene names: 218549344

Gene position: 2064227-2064889 (Clockwise)

Preceding gene: 218549343

Following gene: 218549345

Centisome position: 44.98

GC content: 50.83

Gene sequence:

>663_bases
ATGGAAATTAATGAAAAACTGCTGCGCCAGATAATTGAACAAGTGTTATGTGAAATGCAAACCAGCGATAAGCCGGTTTC
CTTTCAGGCATCCACAGCAACCGCAGCTCCGGCAGCTTCAGACAGCTTTCTGACTGAAATTGGGGAAGCCCGCCAGGGGA
CACAGCAGGATGAAGTCATCATCGCTGTTGGCCCGGCATTTGGTCTTTCACAAACGGTCAATATCGTGGGCTTGTCGCAT
AAAAGCATTCTGCGGGAAGTCATAGCGGGTATCGAAGAAGAAGGCATTAAGGCCAGGGTGATCCGCTGCTTTAAATCTTC
CGATGTGGCCTTTGTCGCGGTTGAAGGTAATCGCCTGAGCGGTTCTGGCATATCTATCGGTATTCAGTCTAAAGGCACCA
CCGTTATTCACCAACAGGGGCTGCCACCGCTGTCTAACCTGGAGTTGTTCCCACAAGCTCCCTTGCTGACGCTGGAAACG
TATCGCCAGATTGGCAAAAACGCTGCGCGCTATGCCAAACGTGAGTCACCGCAACCTGTACCAACGCTTAATGATCAGAT
GGCACGCCCGAAATATCAGGCTAAATCCGCCATTTTGCATATCAAAGAGACCAAGTACGTCGTGACCGGTAAAAACCCGC
AGGAACTGCGCGTGGCGCTTTAA

Upstream 100 bases:

>100_bases
GGTCCGGCAACAGGTTACCGCCTGCAAGGCGAACGCTGGGAAGAGATTAAAAATATCCCTGGCGCACTTGATCCCAACGA
ACTTGGCTAAGGGGTGAAAA

Downstream 100 bases:

>100_bases
TAAAGGATTTCCTCATGAATACCGATGCAATTGAATCGATGGTTCGGGACGTACTGAGCCGCATGAATAGCCTGGAGGGC
GCAGCTCCTGCCCCTGCTCC

Product: Propanediol utilization: diol dehydratase, medium subunit

Products: NA

Alternate protein names: Propanediol utilization protein pduD [H]

Number of amino acids: Translated: 220; Mature: 220

Protein sequence:

>220_residues
MEINEKLLRQIIEQVLCEMQTSDKPVSFQASTATAAPAASDSFLTEIGEARQGTQQDEVIIAVGPAFGLSQTVNIVGLSH
KSILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTLET
YRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL

Sequences:

>Translated_220_residues
MEINEKLLRQIIEQVLCEMQTSDKPVSFQASTATAAPAASDSFLTEIGEARQGTQQDEVIIAVGPAFGLSQTVNIVGLSH
KSILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTLET
YRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL
>Mature_220_residues
MEINEKLLRQIIEQVLCEMQTSDKPVSFQASTATAAPAASDSFLTEIGEARQGTQQDEVIIAVGPAFGLSQTVNIVGLSH
KSILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLSGSGISIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTLET
YRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL

Specific function: Part of the pduCDE complex that catalyzes the dehydration of 1,2-propanediol to propionaldehyde. Is required for S.typhimurium growth on 1,2-propanediol as the sole carbon and energy source [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diol/glycerol dehydratase medium subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010254
- InterPro:   IPR003208 [H]

Pfam domain/function: PF02288 Dehydratase_MU [H]

EC number: =4.2.1.28 [H]

Molecular weight: Translated: 23892; Mature: 23892

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEINEKLLRQIIEQVLCEMQTSDKPVSFQASTATAAPAASDSFLTEIGEARQGTQQDEVI
CCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
IAVGPAFGLSQTVNIVGLSHKSILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLS
EEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCEEC
GSGISIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTLETYRQIGKNAARYAKRESPQPV
CCCEEEEECCCCCEEEEECCCCCCCCCEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
PTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL
CCCCHHHCCCCHHHHHHEEEEECCEEEEECCCHHHEEEEC
>Mature Secondary Structure
MEINEKLLRQIIEQVLCEMQTSDKPVSFQASTATAAPAASDSFLTEIGEARQGTQQDEVI
CCHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEE
IAVGPAFGLSQTVNIVGLSHKSILREVIAGIEEEGIKARVIRCFKSSDVAFVAVEGNRLS
EEECCCCCCCCEEEEEECCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEEECCEEC
GSGISIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTLETYRQIGKNAARYAKRESPQPV
CCCEEEEECCCCCEEEEECCCCCCCCCEECCCCCEEHHHHHHHHHHHHHHHHHCCCCCCC
PTLNDQMARPKYQAKSAILHIKETKYVVTGKNPQELRVAL
CCCCHHHCCCCHHHHHHEEEEECCEEEEECCCHHHEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9352910; 10498708; 11677609 [H]