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The map label for this gene is pflC [H]

Identifier: 218549331

GI number: 218549331

Start: 2053328

End: 2054275

Strand: Direct

Name: pflC [H]

Synonym: EFER_1996

Alternate gene names: 218549331

Gene position: 2053328-2054275 (Clockwise)

Preceding gene: 218549330

Following gene: 218549332

Centisome position: 44.75

GC content: 49.26

Gene sequence:

>948_bases
GTGAGCGCAAATAAAGAATTAAGCGGACGAATTTTCAATATCCAGAAATATTCGATCTACGACGGTGACGGTATCCGCAC
ACTGATTTTTTTTAAAGGCTGCAATCTTCGCTGCCCGTGGTGTGCCAACCCGGAAGGGTTAAGCAGCCAGTTTCAGGTGA
TGTTCTCTCAGGATAAATGCATCAACTGCGGCGACTGCGTCAATGTATGCCCGGCGGGGATCCACTACCGTGCGGAAGTC
AACGGAGAAATGAAGCATTTCGTCAACCGTAATAAAGACTGCATTGGTTGTCGCAAGTGTGAAGAAATTTGCACCCAAAA
TGCACTGGATATCATGGGTAAAGATGTCACGGTCAGTGAACTGATGGAGATCATCATGCAGGACTATGACTTTTACGTCT
CCTCCGGCGGTGGTGTCACTATTGGGGGCGGTGAGATGAGCTTGCAAACTGATTTTGCCGTTGCCCTGTTCAGTGAATGC
AAAAAGATGATGATCAACACCGCCGTTGAAACCCAGGGTACAACCCCGCTTGCAAACTATCAGAAGCTGGCTCCGGTCAC
CGACACCTTCCTGTTCGATATCAAACAGATCGATAGCAATCACCATAAAACGCTGTTCGGAATTGGCAACGAAGGCGTGC
GTCGCAATCTGGAGTGGCTGGTGGATTCGGGTGCCAACGTGATTGTTCGTATGCCGCTGATTCGTGGTTATAACGATTCG
TGGGAAGCGATTACCGGTGCTATCGACTACGTCCAGAAACTGGCCAAACGCGGCAATATTCGCCGAATCGATATGCTGCC
GTACCACCAGCTGGGGCGCAAAAAGTATGAACGACTGGAGATGCCCTACCCGATTGTTGAAGACCCGTCATACAGCGTGG
AAGAGCTGGACAAACTGGAGGCATTTTTCGCCCAGTTCGATTTTGATATTCGCTTAGTTCGTCATTAA

Upstream 100 bases:

>100_bases
GGAAGTTCAGGACGAAATCATCAGCCGTACGGTGATTGAGAAGTTCTGATAAGACGGATCGCAACCGGACAAGGATGTCC
GACATTTTCAGGAGGCAAGC

Downstream 100 bases:

>100_bases
AGGAGCCGTAAATGAACAGTTTAGGCGTAATTGAAACACGTGGTCTTGTAGCCGCAATTCAAGCCGTAGATGCCGCCTGC
AAAGCAGCCGGGGTGAGCTG

Product: pyruvate formate-lyase activating enzyme

Products: NA

Alternate protein names: Formate-C-acetyltransferase-activating enzyme 2; PFL-activating enzyme 2 [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANPEGLSSQFQVMFSQDKCINCGDCVNVCPAGIHYRAEV
NGEMKHFVNRNKDCIGCRKCEEICTQNALDIMGKDVTVSELMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSEC
KKMMINTAVETQGTTPLANYQKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLEWLVDSGANVIVRMPLIRGYNDS
WEAITGAIDYVQKLAKRGNIRRIDMLPYHQLGRKKYERLEMPYPIVEDPSYSVEELDKLEAFFAQFDFDIRLVRH

Sequences:

>Translated_315_residues
MSANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANPEGLSSQFQVMFSQDKCINCGDCVNVCPAGIHYRAEV
NGEMKHFVNRNKDCIGCRKCEEICTQNALDIMGKDVTVSELMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSEC
KKMMINTAVETQGTTPLANYQKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLEWLVDSGANVIVRMPLIRGYNDS
WEAITGAIDYVQKLAKRGNIRRIDMLPYHQLGRKKYERLEMPYPIVEDPSYSVEELDKLEAFFAQFDFDIRLVRH
>Mature_314_residues
SANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANPEGLSSQFQVMFSQDKCINCGDCVNVCPAGIHYRAEVN
GEMKHFVNRNKDCIGCRKCEEICTQNALDIMGKDVTVSELMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSECK
KMMINTAVETQGTTPLANYQKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLEWLVDSGANVIVRMPLIRGYNDSW
EAITGAIDYVQKLAKRGNIRRIDMLPYHQLGRKKYERLEMPYPIVEDPSYSVEELDKLEAFFAQFDFDIRLVRH

Specific function: Activation of pyruvate formate-lyase 2 under anaerobic conditions by generation of an organic free radical, using S- adenosylmethionine and reduced flavodoxin as cosubstrates to produce 5'-deoxy-adenosine [H]

COG id: COG1180

COG function: function code O; Pyruvate-formate lyase-activating enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI1790389, Length=284, Percent_Identity=35.9154929577465, Blast_Score=191, Evalue=5e-50,
Organism=Escherichia coli, GI226510931, Length=296, Percent_Identity=34.4594594594595, Blast_Score=150, Evalue=9e-38,
Organism=Escherichia coli, GI1790839, Length=249, Percent_Identity=28.9156626506024, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI1787130, Length=198, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006638
- InterPro:   IPR012839
- InterPro:   IPR011352
- InterPro:   IPR001989
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =1.97.1.4 [H]

Molecular weight: Translated: 35749; Mature: 35618

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS01087 RADICAL_ACTIVATING ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
7.6 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
7.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANPEGLSSQFQVMFSQDKC
CCCCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCEECCCCCCCCCCCCCEEEEECCCCC
INCGDCVNVCPAGIHYRAEVNGEMKHFVNRNKDCIGCRKCEEICTQNALDIMGKDVTVSE
CCCCCHHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHH
LMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSECKKMMINTAVETQGTTPLANY
HHHHHHHCCCEEEECCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLEWLVDSGANVIVRMPLIRGYNDS
HHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
WEAITGAIDYVQKLAKRGNIRRIDMLPYHQLGRKKYERLEMPYPIVEDPSYSVEELDKLE
HHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AFFAQFDFDIRLVRH
HHHHHCCCEEEEECC
>Mature Secondary Structure 
SANKELSGRIFNIQKYSIYDGDGIRTLIFFKGCNLRCPWCANPEGLSSQFQVMFSQDKC
CCCCCCCCEEEEEEEEEEECCCCCEEEEEEECCCEECCCCCCCCCCCCCEEEEECCCCC
INCGDCVNVCPAGIHYRAEVNGEMKHFVNRNKDCIGCRKCEEICTQNALDIMGKDVTVSE
CCCCCHHHHCCCCEEEEEECCCHHHHHHCCCCCCCHHHHHHHHHHCCHHHHCCCCCCHHH
LMEIIMQDYDFYVSSGGGVTIGGGEMSLQTDFAVALFSECKKMMINTAVETQGTTPLANY
HHHHHHHCCCEEEECCCCEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QKLAPVTDTFLFDIKQIDSNHHKTLFGIGNEGVRRNLEWLVDSGANVIVRMPLIRGYNDS
HHHCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
WEAITGAIDYVQKLAKRGNIRRIDMLPYHQLGRKKYERLEMPYPIVEDPSYSVEELDKLE
HHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHH
AFFAQFDFDIRLVRH
HHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]