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The map label for this gene is ycdW

Identifier: 218549237

GI number: 218549237

Start: 1939077

End: 1940015

Strand: Reverse

Name: ycdW

Synonym: EFER_1897

Alternate gene names: 218549237

Gene position: 1940015-1939077 (Counterclockwise)

Preceding gene: 218549238

Following gene: 218549236

Centisome position: 42.28

GC content: 51.44

Gene sequence:

>939_bases
ATGGATATCCTTTTTTATCACCCAACGTTCGACACCACATGGTGGATTAATGCCCTGAGAGAGGCGTTGCCTGAGGCAAA
AGTGCGTGAGTGGAAATGCGGCGATCACGATCCAGCTGATTATGCGCTGGTCTGGCATCCGCCCGTTGAGATGCTGGCTG
GGCGAACATTGAAGGCTGTGTTTGCTCTCGGTGCCGGTGTCGATTCGATTTTGAGTAAGTTACAGGCGCATCCTGAAATG
TTAGCCCCCTCCGTCCCACTGTTTCGACTCGAAGATACCGGGATGGGCGAGCAGATGCAGGAATATGCCGTAAGCCAGGT
GTTGCACTGGTTTCGTCGGTTTGATGATTATCAGGCACTGAAGAATGAATCTCGTTGGGAACCCCTGCCAGAATATCGCA
AGGAAGATTTTAGTATCGGGATCATGGGCGCAGGTGTGCTGGGCAGTAAAGTAGCAGAAAGCCTGAAAGTCTGGGGATTC
CCGCTACGTTGCTGGAGTCGTAGCCGAAAATCCTGGCCAGGCGTCGAAAGTTTTGCCGGGAAAGAGGAGTTGGGCGCTTT
TCTGCGTAAGACGCGAGTATTAATTAACCTGTTACCCAACACGCCAGAAACCGTCGGTATTATTAATCTGCATTTGCTCA
ACCAACTGCAGGACAATGCATACCTGCTTAACCTCGCGCGGGGTGTACATGTTGTCGAAGATGACCTGCTGATTGCCCTG
AATAACGGCAAACTGAAAGGTGCGATGCTGGATGTTTTTAGTCGGGAACCTCTGCCAGCCGAAAGCCCGTTGTGGAAACA
TCCGCGCGTGGCGATGACACCACATGTCGCAGCAGTAACACGTCCGGCTGAAGCTGTGGCGTATATTTCAAAAACGATTA
ACCGTCTTGAGAAAGGTGAACCTGTTACCGGTCAAGTTGATCGTATCCGTGGCTACTGA

Upstream 100 bases:

>100_bases
TTTTTTTGCCACCGGCAGTGAATTTGTTATGCTGATGGCTCTAAATAATTATGACAATAAGTGCGAGTAAGTTTTGCACA
ACAACCCTCAGGAGTGAGTT

Downstream 100 bases:

>100_bases
TGACAACCCGGTGAAAGCCGGGTTTCAACTAATAAACACCTGCGATTCCTGCTATCCTTGTCGCAAAATGACTACCGGAG
AGAACTATGTATCCCGTCGA

Product: 2-ketoacid reductase

Products: NA

Alternate protein names: 2-ketoacid reductase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDILFYHPTFDTTWWINALREALPEAKVREWKCGDHDPADYALVWHPPVEMLAGRTLKAVFALGAGVDSILSKLQAHPEM
LAPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYQALKNESRWEPLPEYRKEDFSIGIMGAGVLGSKVAESLKVWGF
PLRCWSRSRKSWPGVESFAGKEELGAFLRKTRVLINLLPNTPETVGIINLHLLNQLQDNAYLLNLARGVHVVEDDLLIAL
NNGKLKGAMLDVFSREPLPAESPLWKHPRVAMTPHVAAVTRPAEAVAYISKTINRLEKGEPVTGQVDRIRGY

Sequences:

>Translated_312_residues
MDILFYHPTFDTTWWINALREALPEAKVREWKCGDHDPADYALVWHPPVEMLAGRTLKAVFALGAGVDSILSKLQAHPEM
LAPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYQALKNESRWEPLPEYRKEDFSIGIMGAGVLGSKVAESLKVWGF
PLRCWSRSRKSWPGVESFAGKEELGAFLRKTRVLINLLPNTPETVGIINLHLLNQLQDNAYLLNLARGVHVVEDDLLIAL
NNGKLKGAMLDVFSREPLPAESPLWKHPRVAMTPHVAAVTRPAEAVAYISKTINRLEKGEPVTGQVDRIRGY
>Mature_312_residues
MDILFYHPTFDTTWWINALREALPEAKVREWKCGDHDPADYALVWHPPVEMLAGRTLKAVFALGAGVDSILSKLQAHPEM
LAPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYQALKNESRWEPLPEYRKEDFSIGIMGAGVLGSKVAESLKVWGF
PLRCWSRSRKSWPGVESFAGKEELGAFLRKTRVLINLLPNTPETVGIINLHLLNQLQDNAYLLNLARGVHVVEDDLLIAL
NNGKLKGAMLDVFSREPLPAESPLWKHPRVAMTPHVAAVTRPAEAVAYISKTINRLEKGEPVTGQVDRIRGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily

Homologues:

Organism=Homo sapiens, GI6912396, Length=265, Percent_Identity=27.1698113207547, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI145580578, Length=240, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI4557499, Length=240, Percent_Identity=27.0833333333333, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI23308577, Length=263, Percent_Identity=26.615969581749, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4557497, Length=243, Percent_Identity=27.9835390946502, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI61743967, Length=243, Percent_Identity=27.9835390946502, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI145580575, Length=236, Percent_Identity=26.6949152542373, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI87081824, Length=312, Percent_Identity=81.4102564102564, Blast_Score=536, Evalue=1e-154,
Organism=Escherichia coli, GI87082289, Length=165, Percent_Identity=28.4848484848485, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=283, Percent_Identity=26.5017667844523, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324964, Length=209, Percent_Identity=25.8373205741627, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324055, Length=179, Percent_Identity=28.4916201117318, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28574286, Length=266, Percent_Identity=29.3233082706767, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24585514, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574282, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552429, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28574284, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45551003, Length=240, Percent_Identity=26.25, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24585516, Length=234, Percent_Identity=26.9230769230769, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GHRA_ESCF3 (B7LTA2)

Other databases:

- EMBL:   CU928158
- RefSeq:   YP_002383028.1
- EnsemblBacteria:   EBESCT00000122408
- GeneID:   7119914
- GenomeReviews:   CU928158_GR
- KEGG:   efe:EFER_1897
- GeneTree:   EBGT00050000009080
- HOGENOM:   HBG731446
- ProtClustDB:   PRK15469
- BioCyc:   EFER585054:EFER_1897-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01666
- InterPro:   IPR006140
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720

Pfam domain/function: PF02826 2-Hacid_dh_C

EC number: =1.1.1.79; =1.1.1.81

Molecular weight: Translated: 35230; Mature: 35230

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1; PS00670 D_2_HYDROXYACID_DH_2; PS00671 D_2_HYDROXYACID_DH_3

Important sites: ACT_SITE 227-227 ACT_SITE 275-275

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILFYHPTFDTTWWINALREALPEAKVREWKCGDHDPADYALVWHPPVEMLAGRTLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEECCCHHHHHCCHHHHH
FALGAGVDSILSKLQAHPEMLAPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYQAL
HHHHCCHHHHHHHHHCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KNESRWEPLPEYRKEDFSIGIMGAGVLGSKVAESLKVWGFPLRCWSRSRKSWPGVESFAG
HCCCCCCCCHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHCC
KEELGAFLRKTRVLINLLPNTPETVGIINLHLLNQLQDNAYLLNLARGVHVVEDDLLIAL
HHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCEEEEHHCCCEEECCCEEEEE
NNGKLKGAMLDVFSREPLPAESPLWKHPRVAMTPHVAAVTRPAEAVAYISKTINRLEKGE
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHCCC
PVTGQVDRIRGY
CCCCCHHHHCCC
>Mature Secondary Structure
MDILFYHPTFDTTWWINALREALPEAKVREWKCGDHDPADYALVWHPPVEMLAGRTLKAV
CCEEEECCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCEEEEECCCHHHHHCCHHHHH
FALGAGVDSILSKLQAHPEMLAPSVPLFRLEDTGMGEQMQEYAVSQVLHWFRRFDDYQAL
HHHHCCHHHHHHHHHCCHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KNESRWEPLPEYRKEDFSIGIMGAGVLGSKVAESLKVWGFPLRCWSRSRKSWPGVESFAG
HCCCCCCCCHHHCCCCCEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCCCHHHHCC
KEELGAFLRKTRVLINLLPNTPETVGIINLHLLNQLQDNAYLLNLARGVHVVEDDLLIAL
HHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHCCCCEEEEHHCCCEEECCCEEEEE
NNGKLKGAMLDVFSREPLPAESPLWKHPRVAMTPHVAAVTRPAEAVAYISKTINRLEKGE
CCCCCCHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHHHHHHCCC
PVTGQVDRIRGY
CCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA