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The map label for this gene is cpsA1 [H]

Identifier: 218549134

GI number: 218549134

Start: 1838898

End: 1840199

Strand: Reverse

Name: cpsA1 [H]

Synonym: EFER_1786

Alternate gene names: 218549134

Gene position: 1840199-1838898 (Counterclockwise)

Preceding gene: 218549136

Following gene: 218549133

Centisome position: 40.1

GC content: 45.39

Gene sequence:

>1302_bases
GTGAAACTTAATAAAGCCACACTGTTTACCTCGTTATTATTCTGTACGGGATTATCGTTTGCATCAACAACATGGATGGA
AGAGGGCATCAATAAAAACTACGCCGAAACTGTTCAGTTGCGCAGAGATATACACCGGCATCCAGAGTTGGGTAATCAGG
AAATAAATACCCAACAAAAAGTGGTTAATTACCTTAAACAACAAGGTATAGAGGTCGTAACCGGTTGGAAAAATGCACCT
ACCGCTGTGATTGGCATCATTAACCCTGATAAAAAGAACACAGTTGCTCTGCGTAGTGAGCTTGACGCTTTGCCGATTAA
AGAAAACACCGGTTTGTCATTTGCGAGTCAGAGTACCGGGCAATTTATGGGGCAAGAAGTGCCGGTATCACATATGTGTG
GTCACGACGTGCATATGGCAATGTTGCTTTCTGCAGCAAAAATATTGCAACAGAACAAAGACAAGTTTAATAACCGGGTA
GTGTTAATTTTCCAGCCAGCGGAAGAGGGCGACAGTATCCATGATCTTTTTGATGAGAATCTTCCTGTAAGCGGGGCCAA
TGCTCTTGTGCAGGATGGCTTAATGGAAAAATATGCCATTAAGCGCGTGTTTGGTATTCACGTTATGGCAAGGCTACCTG
CAGGGAAAATGTGGATTGCGCAAGGACCGGCGTTGAATAGTGTCGATGATTTCATTATTAAGGTTGAAGGTAAACAGTCT
CATGGTGCGATGCCGTGGGCCGGAAGTGATGCCACGCTTACAGCTGCAAACATTGTTGTCGCGTTACAACAGATTGTCAG
CCGTAATGCTGATTTATCCTCGGGAATGGGCGTAATCACTGTGGGCAAATTGACCGCAGGTGAAACCGCAAATGTGATGA
GCGGGAAAGCGGAGATGGTTGGTACAATTCGCAGTAATAATGCCGATATTCGTCAAACCTTGTTAACGCGCATTCCTCAG
GTTGCCGAAGGTATTGCGGACGCGGCCGGAGCGAAAGCTATAACGCAAATTGTGAAAATATATCCAGTGACAATGAATAA
TCCGCAGTTGGTCGAGACGATGGTTCCGTTGCTGCAAAAACAGGGCATTGATGCTGAGATCAACAACTGGAATCCTGGCG
CATCTGAAGACTTCAGTTTTTATGCCCAAAAAGCACCGGGTATGTTTATGTTTCTCGGTGCTGATGCTGCCGGAACGTCT
GATGTACAAAATAATCACAGTGATAAATTTAACGTAGATGAAACCACCATGCGCACGGGGGTGATGGCTCATCTGGTAGC
AGCAACGTCGGAGCTTGAGTAA

Upstream 100 bases:

>100_bases
TGATATTTGCATGTCGTGTTTAATAATTTGATTTTTATTGACTATTTTCAGGTTTTACGCAAATCTCAATTGCACAATAA
AATAACCATCTGGAGATTAT

Downstream 100 bases:

>100_bases
CATCTGTTGACACTACATTAAAATACAGTAATACCTGCCGAGAAATTCGGCAGGTTACTTTTCAGAGCTCTCAGAGATTT
ATATTTCAACAGTATAGTTA

Product: Thermostable carboxypeptidase 1

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MKLNKATLFTSLLFCTGLSFASTTWMEEGINKNYAETVQLRRDIHRHPELGNQEINTQQKVVNYLKQQGIEVVTGWKNAP
TAVIGIINPDKKNTVALRSELDALPIKENTGLSFASQSTGQFMGQEVPVSHMCGHDVHMAMLLSAAKILQQNKDKFNNRV
VLIFQPAEEGDSIHDLFDENLPVSGANALVQDGLMEKYAIKRVFGIHVMARLPAGKMWIAQGPALNSVDDFIIKVEGKQS
HGAMPWAGSDATLTAANIVVALQQIVSRNADLSSGMGVITVGKLTAGETANVMSGKAEMVGTIRSNNADIRQTLLTRIPQ
VAEGIADAAGAKAITQIVKIYPVTMNNPQLVETMVPLLQKQGIDAEINNWNPGASEDFSFYAQKAPGMFMFLGADAAGTS
DVQNNHSDKFNVDETTMRTGVMAHLVAATSELE

Sequences:

>Translated_433_residues
MKLNKATLFTSLLFCTGLSFASTTWMEEGINKNYAETVQLRRDIHRHPELGNQEINTQQKVVNYLKQQGIEVVTGWKNAP
TAVIGIINPDKKNTVALRSELDALPIKENTGLSFASQSTGQFMGQEVPVSHMCGHDVHMAMLLSAAKILQQNKDKFNNRV
VLIFQPAEEGDSIHDLFDENLPVSGANALVQDGLMEKYAIKRVFGIHVMARLPAGKMWIAQGPALNSVDDFIIKVEGKQS
HGAMPWAGSDATLTAANIVVALQQIVSRNADLSSGMGVITVGKLTAGETANVMSGKAEMVGTIRSNNADIRQTLLTRIPQ
VAEGIADAAGAKAITQIVKIYPVTMNNPQLVETMVPLLQKQGIDAEINNWNPGASEDFSFYAQKAPGMFMFLGADAAGTS
DVQNNHSDKFNVDETTMRTGVMAHLVAATSELE
>Mature_433_residues
MKLNKATLFTSLLFCTGLSFASTTWMEEGINKNYAETVQLRRDIHRHPELGNQEINTQQKVVNYLKQQGIEVVTGWKNAP
TAVIGIINPDKKNTVALRSELDALPIKENTGLSFASQSTGQFMGQEVPVSHMCGHDVHMAMLLSAAKILQQNKDKFNNRV
VLIFQPAEEGDSIHDLFDENLPVSGANALVQDGLMEKYAIKRVFGIHVMARLPAGKMWIAQGPALNSVDDFIIKVEGKQS
HGAMPWAGSDATLTAANIVVALQQIVSRNADLSSGMGVITVGKLTAGETANVMSGKAEMVGTIRSNNADIRQTLLTRIPQ
VAEGIADAAGAKAITQIVKIYPVTMNNPQLVETMVPLLQKQGIDAEINNWNPGASEDFSFYAQKAPGMFMFLGADAAGTS
DVQNNHSDKFNVDETTMRTGVMAHLVAATSELE

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=390, Percent_Identity=25.3846153846154, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 46906; Mature: 46906

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLNKATLFTSLLFCTGLSFASTTWMEEGINKNYAETVQLRRDIHRHPELGNQEINTQQK
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
VVNYLKQQGIEVVTGWKNAPTAVIGIINPDKKNTVALRSELDALPIKENTGLSFASQSTG
HHHHHHHCCCEEEECCCCCCEEEEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCHH
QFMGQEVPVSHMCGHDVHMAMLLSAAKILQQNKDKFNNRVVLIFQPAEEGDSIHDLFDEN
HHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHCC
LPVSGANALVQDGLMEKYAIKRVFGIHVMARLPAGKMWIAQGPALNSVDDFIIKVEGKQS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCEEEEEECCCC
HGAMPWAGSDATLTAANIVVALQQIVSRNADLSSGMGVITVGKLTAGETANVMSGKAEMV
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCHHHE
GTIRSNNADIRQTLLTRIPQVAEGIADAAGAKAITQIVKIYPVTMNNPQLVETMVPLLQK
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
QGIDAEINNWNPGASEDFSFYAQKAPGMFMFLGADAAGTSDVQNNHSDKFNVDETTMRTG
CCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
VMAHLVAATSELE
HHHHHHHHHHCCC
>Mature Secondary Structure
MKLNKATLFTSLLFCTGLSFASTTWMEEGINKNYAETVQLRRDIHRHPELGNQEINTQQK
CCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHH
VVNYLKQQGIEVVTGWKNAPTAVIGIINPDKKNTVALRSELDALPIKENTGLSFASQSTG
HHHHHHHCCCEEEECCCCCCEEEEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCCCCCHH
QFMGQEVPVSHMCGHDVHMAMLLSAAKILQQNKDKFNNRVVLIFQPAEEGDSIHDLFDEN
HHCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHCC
LPVSGANALVQDGLMEKYAIKRVFGIHVMARLPAGKMWIAQGPALNSVDDFIIKVEGKQS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCCCCCCCCEEEEEECCCC
HGAMPWAGSDATLTAANIVVALQQIVSRNADLSSGMGVITVGKLTAGETANVMSGKAEMV
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCHHHCCCHHHE
GTIRSNNADIRQTLLTRIPQVAEGIADAAGAKAITQIVKIYPVTMNNPQLVETMVPLLQK
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHH
QGIDAEINNWNPGASEDFSFYAQKAPGMFMFLGADAAGTSDVQNNHSDKFNVDETTMRTG
CCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHH
VMAHLVAATSELE
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]