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The map label for this gene is prmC [H]

Identifier: 218549097

GI number: 218549097

Start: 1798061

End: 1798891

Strand: Reverse

Name: prmC [H]

Synonym: EFER_1749

Alternate gene names: 218549097

Gene position: 1798891-1798061 (Counterclockwise)

Preceding gene: 218549098

Following gene: 218549096

Centisome position: 39.2

GC content: 54.39

Gene sequence:

>831_bases
ATGGAATATCAACACTGGCTGCGTGAAGCCATTAACCAATTACAGGGAAGCGAAAGCCCACGCCGTGACGCTGAAATCTT
ATTGGAGTATGTCACTGGCAGGGGGCGAACTTTTATTCTCGCCTTTGGCGAAACGCAACTGACTGATGAACAACTTCAGC
AACTTGACGCGCTACTGGCGCGTCGTCGCGCTGGAGAACCTGTCGCCCATTTGACGGGGCAGCGTGAATTCTGGTCACTG
CCGTTATTTGTATCGCCAGCCACCTTGATTCCGCGCCCGGACACCGAATGTCTGGTGGAACAGGCACTGGAGCGCCTACC
TGAACAACCCTGTCGCATTCTCGATCTCGGTACGGGCACCGGGGCGATTGCGCTTGCGCTTGCCAGCGAGCGCCCGGACT
GCGAAATTACCGCTGTAGATCGCATGCCCGATGCTGTCGCTCTGGCACAACGTAATGCGCAGCATCTGGCAATCAAAAAT
ATCCACATTCTGCAAAGCGACTGGTTTAGCGCGCTAGCCGGGCAGCAGTTTGCGATGATTGTCAGTAACCCACCGTATAT
CGACGAGCAGGACCCGCATCTTCAACAAGGTGACGTGCGCTTTGAGCCACTCACGGCGTTAGTTGCAGGTGATCACGGGA
TGGCGGATATTGTGCATATTATTGAGCACTCCCGTGCAGCTCTGGCGAAAGGGGGGTATTTATTGCTGGAGCACGGCTGG
CAGCAGGGAGCGGCAGTAAGACAGGCATTTATCCAGGCAGGATACAGTAATGTAGAAACCTGCAGGGATTATGGCGGTAA
TGAACGAATTACCCTCGGACGTTTGCTTTAA

Upstream 100 bases:

>100_bases
GCCTGGATGAAGTGATGGAAGGTAAGCTGGATATGCTGATTGAACCGATTATCCAGGAACATCAGGCCGACCAACTGGCG
GCGTTGTCCGAGCAGGAATA

Downstream 100 bases:

>100_bases
GCGCACATAGTGTTATTTCCATAACCTCTGTATGGCTGACTGAGACGCAGTTTGGGGTTATCATTTAAAGAGTTTTGGGT
TTACAAGCGCATGATTACCA

Product: N5-glutamine S-adenosyl-L-methionine-dependent methyltransferase

Products: NA

Alternate protein names: M.EcoKHemKP; Protein-(glutamine-N(5)) MTase hemK; Protein-glutamine N-methyltransferase hemK [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MEYQHWLREAINQLQGSESPRRDAEILLEYVTGRGRTFILAFGETQLTDEQLQQLDALLARRRAGEPVAHLTGQREFWSL
PLFVSPATLIPRPDTECLVEQALERLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKN
IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAGDHGMADIVHIIEHSRAALAKGGYLLLEHGW
QQGAAVRQAFIQAGYSNVETCRDYGGNERITLGRLL

Sequences:

>Translated_276_residues
MEYQHWLREAINQLQGSESPRRDAEILLEYVTGRGRTFILAFGETQLTDEQLQQLDALLARRRAGEPVAHLTGQREFWSL
PLFVSPATLIPRPDTECLVEQALERLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKN
IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAGDHGMADIVHIIEHSRAALAKGGYLLLEHGW
QQGAAVRQAFIQAGYSNVETCRDYGGNERITLGRLL
>Mature_276_residues
MEYQHWLREAINQLQGSESPRRDAEILLEYVTGRGRTFILAFGETQLTDEQLQQLDALLARRRAGEPVAHLTGQREFWSL
PLFVSPATLIPRPDTECLVEQALERLPEQPCRILDLGTGTGAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKN
IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAGDHGMADIVHIIEHSRAALAKGGYLLLEHGW
QQGAAVRQAFIQAGYSNVETCRDYGGNERITLGRLL

Specific function: Methylates the translation termination release factor RF1/PrfA on 'Gln-235' and RF2/PrfB on 'Gln-252' [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=252, Percent_Identity=33.7301587301587, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787463, Length=274, Percent_Identity=91.2408759124088, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI87082085, Length=182, Percent_Identity=37.3626373626374, Blast_Score=107, Evalue=7e-25,
Organism=Saccharomyces cerevisiae, GI6324266, Length=156, Percent_Identity=29.4871794871795, Blast_Score=66, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24582226, Length=236, Percent_Identity=30.5084745762712, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 2.1.1.-

Molecular weight: Translated: 30660; Mature: 30660

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEYQHWLREAINQLQGSESPRRDAEILLEYVTGRGRTFILAFGETQLTDEQLQQLDALLA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHH
RRRAGEPVAHLTGQREFWSLPLFVSPATLIPRPDTECLVEQALERLPEQPCRILDLGTGT
HHHCCCCHHHHCCCHHHEECCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCC
GAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKNIHILQSDWFSALAGQQFAMI
CEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEEEHHHHHHHHCCCCEEEE
VSNPPYIDEQDPHLQQGDVRFEPLTALVAGDHGMADIVHIIEHSRAALAKGGYLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
QQGAAVRQAFIQAGYSNVETCRDYGGNERITLGRLL
CCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCC
>Mature Secondary Structure
MEYQHWLREAINQLQGSESPRRDAEILLEYVTGRGRTFILAFGETQLTDEQLQQLDALLA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHH
RRRAGEPVAHLTGQREFWSLPLFVSPATLIPRPDTECLVEQALERLPEQPCRILDLGTGT
HHHCCCCHHHHCCCHHHEECCEEECCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCC
GAIALALASERPDCEITAVDRMPDAVALAQRNAQHLAIKNIHILQSDWFSALAGQQFAMI
CEEEEEEECCCCCCEEEEECCCCHHHHHHHCCCCCEEEEEEEEEHHHHHHHHCCCCEEEE
VSNPPYIDEQDPHLQQGDVRFEPLTALVAGDHGMADIVHIIEHSRAALAKGGYLLLEHGW
ECCCCCCCCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEECCC
QQGAAVRQAFIQAGYSNVETCRDYGGNERITLGRLL
CCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7883187; 7543480; 11805295; 8905232; 9278503 [H]