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The map label for this gene is purU [H]

Identifier: 218549073

GI number: 218549073

Start: 1771443

End: 1772285

Strand: Direct

Name: purU [H]

Synonym: EFER_1723

Alternate gene names: 218549073

Gene position: 1771443-1772285 (Clockwise)

Preceding gene: 218549072

Following gene: 218549086

Centisome position: 38.6

GC content: 47.69

Gene sequence:

>843_bases
ATGCATTCACTACAACGAAAAGTTCTGCGTACTATTTGCCCTGATCAAAAAGGCCTTATCGCGCGTATTACCAATATTTG
CTACAAGCACGAGTTAAATATCGTGCAGAACAATGAATTTGTTGATCACCGTACCGGGCGCTTTTTTATGCGCACGGAAC
TGGAAGGGATTTTTAATGATTCCACCCTGCTGGCGGATCTCGATAGCGCATTGCCAGAAGGCTCCGTGCGTGAACTGAAT
CCCGCCGGTCGTCGCCGGATAGTGATTCTGGTCACTAAAGAAGCGCATTGCCTGGGCGATCTGCTAATGAAAGCCAACTA
CGGTGGCCTGGATGTTGAAATCGCGGCGGTTATTGGTAACCACGATACCCTGCGTCCATTGGTGGAACGATTTGATATAC
CGTTTGAACTGGTAAGCCATGAAGGGTTAAGCCGCAACGAGCACGATCAGAAGATGGCGGATGCCATTGAAGCACATCAA
CCTGATTATGTCGTGCTGGCGAAATATATGCGGGTATTGACGCCGGAGTTTGTCGCTCGTTTCCCGAACAAAATTATCAA
TATTCACCATTCATTCCTGCCTGCGTTTATTGGTGCACGTCCCTATCATCAGGCTTATGAACGCGGCGTAAAGATTATTG
GCGCAACGGCACACTACGTGAATGACAATCTGGATGAAGGCCCAATCATTATGCAGGACGTTATTCATGTAGATCACACT
TACACTGCCGAAGATATGATGCGTGCGGGTCGTGATGTCGAGAAAAACGTATTAAGCCGCGCGCTTTACCAGGTTTTGGC
GCAGCGTGTATTTGTTTACGGTAACCGGACGATTATTCTTTAA

Upstream 100 bases:

>100_bases
TGCCCTTGTGGTTCAGGTAAAAAATTTAAAAAGTGCTGCGGCCAATAACAGCTGACGGCATGTAACCATGCAAACACTCT
CTTACAAGGTTTTACCAGCA

Downstream 100 bases:

>100_bases
TCGCTGGCAAAATAGAACTGGTTGCCTTTAATCCGTTACGGATGAAAATTACGCAACCAGTTCATTTTTCTCAACGTAAC
CCTTTACAGCGGCGTGTCAT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRPLVERFDIPFELVSHEGLSRNEHDQKMADAIEAHQ
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYQVLAQRVFVYGNRTIIL

Sequences:

>Translated_280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRPLVERFDIPFELVSHEGLSRNEHDQKMADAIEAHQ
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYQVLAQRVFVYGNRTIIL
>Mature_280_residues
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELN
PAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGNHDTLRPLVERFDIPFELVSHEGLSRNEHDQKMADAIEAHQ
PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
YTAEDMMRAGRDVEKNVLSRALYQVLAQRVFVYGNRTIIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=30, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=30, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=30, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1787483, Length=280, Percent_Identity=98.2142857142857, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1788846, Length=94, Percent_Identity=40.4255319148936, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=31.5508021390374, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=30.188679245283, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 31919; Mature: 31919

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFND
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHCCC
STLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGN
CHHHHHHHHHCCCCCCHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECC
HDTLRPLVERFDIPFELVSHEGLSRNEHDQKMADAIEAHQPDYVVLAKYMRVLTPEFVAR
CHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHH
FPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHCCCC
YTAEDMMRAGRDVEKNVLSRALYQVLAQRVFVYGNRTIIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHRTGRFFMRTELEGIFND
CCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEECCCCCCCCCCCEEEEEHHHHHCCC
STLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLLMKANYGGLDVEIAAVIGN
CHHHHHHHHHCCCCCCHHCCCCCCEEEEEEEECCHHHHHHHHHHCCCCCCCEEEEEEECC
HDTLRPLVERFDIPFELVSHEGLSRNEHDQKMADAIEAHQPDYVVLAKYMRVLTPEFVAR
CHHHHHHHHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHH
FPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIMQDVIHVDHT
CCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEEEHHHHCCCCCCCCHHHHHHHHCCCC
YTAEDMMRAGRDVEKNVLSRALYQVLAQRVFVYGNRTIIL
CCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]