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The map label for this gene is cls [H]

Identifier: 218549057

GI number: 218549057

Start: 1751082

End: 1752542

Strand: Direct

Name: cls [H]

Synonym: EFER_1705

Alternate gene names: 218549057

Gene position: 1751082-1752542 (Clockwise)

Preceding gene: 218549056

Following gene: 218549058

Centisome position: 38.16

GC content: 48.67

Gene sequence:

>1461_bases
ATGACAACCTTCTACACGGTGGTGAGTTGGCTGGTCATTCTGGGATACTGGTTACTCATCGCTGGCGTAACATTACGTAT
TCTAATGAAACGTCGTGCAGTTCCCTCCGCAATGGCCTGGTTGCTGATTATCTACATCCTGCCGCTGGTGGGCATTATTG
CTTATCTCTCTTTTGGTGAACTTCATTTAGGTAAACGCCGTGCTGAACGAGCCAGGGCGATGTGGCCTTCAACCGCAAAA
TGGCTTAACGACCTGAAATCCTGCAAGCACATATTTGCAGAAGAGAACAGTAGTGTTGCTTCTTCTTTATTTAAGTTGTG
TGAACGTCGCCAGGGGATCGCAGGAGTAAAAGGTAACCAATTACAACTGCTCACCGATTCTGATGATGTGATGCAAGCTC
TAATCCGCGATATTCAGCTTGCCCGCCATAATATTGAGATGGTGTTTTATATCTGGCAGCCTGGTGGCATGGCCGATAAA
GTCGCTGAGTCATTAATGGCTGCAGCTCGTCGTGGTATTCATTGTCGACTGATGCTTGATTCTGCTGGCAGTGTGGCTTT
TTTCCGTAGCCCATGGGCAGCCATGATGCGAAATGCAGGTATTGAAGTAGTCGAGGCGCTGAAAGTTAACCTGATGCGTG
TCTTTTTGCGACGCATGGACCTTCGCCAGCACCGCAAAATGATCATGATCGACAATTATATCGCTTACACTGGCAGCATG
AATATGGTCGATCCGCGCTTCTTTAAACAAGATGCCGGTGTAGGCCAGTGGGTAGATGTCATGGCGCGCATGGAAGGCCC
CATCGCGACAGCTATGGGCGTGATTTATTCCTGCGACTGGGAAATTGAAACCGGTAAACGCATTCTGCCACCGCCGCCTG
ACGTCAATATCATGCCATTTGAGCAAGCCAGCGGTCATACCATTCACACCATTGCTTCTGGCCCTGGCTTCCCGGAGGAT
TTGATTCACCAGGCCTTGCTGACTGCAACATATTCAGCTCGTGAATATTTAATTATGACGACACCCTACTTCGTGCCCAG
TGATGACTTGCTACATGCAATTTGTACTGCTGCACAGCGTGGCGTGGACGTCAGCATTATCCTGCCACGTAAAAATGACT
CTATGCTGGTTGGTTGGGCCAGTCGTGCTTTCTTCACCGAATTGCTGGCAGCCGGAGTTAAAATTTACCAGTTTGAAGGT
GGTTTATTACATACCAAGAGCGTACTGGTTGATGGTGAGCTTAGTCTTGTCGGTACAGTTAATCTGGATATGCGCAGTTT
GTGGCTTAATTTTGAAATCACACTCGCCATTGACGACAAAGGATTCGGCGCTGATCTCGCCGCAGTACAGGATGACTATA
TCTCCCGTTCCCGGCTTCTTGATGAACGGTTGTGGTTAAAACGACCACTCTGGCAGCGAGTCGCAGAGCGATTGTTTTAC
TTCTTCAGCCCGTTGCTGTAA

Upstream 100 bases:

>100_bases
ACATCATGAGCAAACCAGTTAATTTCCCCTGTTTTTATTTGCCAGATGAATCCTTATGCGTAAACTCATAACAATACGCT
TTCAAAAGGATTTTTAATCT

Downstream 100 bases:

>100_bases
AACGTGCCAAACGAAGTTAACCAGGTAGTCATTATGGATATGGATCTGAACAATCGCCTGACTGAAGATGAAACGCTTGA
GCAGGCTTATGATATTTTTC

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTFYTVVSWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARAMWPSTAK
WLNDLKSCKHIFAEENSSVASSLFKLCERRQGIAGVKGNQLQLLTDSDDVMQALIRDIQLARHNIEMVFYIWQPGGMADK
VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
NMVDPRFFKQDAGVGQWVDVMARMEGPIATAMGVIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPED
LIHQALLTATYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEG
GLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDERLWLKRPLWQRVAERLFY
FFSPLL

Sequences:

>Translated_486_residues
MTTFYTVVSWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARAMWPSTAK
WLNDLKSCKHIFAEENSSVASSLFKLCERRQGIAGVKGNQLQLLTDSDDVMQALIRDIQLARHNIEMVFYIWQPGGMADK
VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
NMVDPRFFKQDAGVGQWVDVMARMEGPIATAMGVIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPED
LIHQALLTATYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEG
GLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDERLWLKRPLWQRVAERLFY
FFSPLL
>Mature_485_residues
TTFYTVVSWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGELHLGKRRAERARAMWPSTAKW
LNDLKSCKHIFAEENSSVASSLFKLCERRQGIAGVKGNQLQLLTDSDDVMQALIRDIQLARHNIEMVFYIWQPGGMADKV
AESLMAAARRGIHCRLMLDSAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSMN
MVDPRFFKQDAGVGQWVDVMARMEGPIATAMGVIYSCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDL
IHQALLTATYSAREYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGG
LLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDERLWLKRPLWQRVAERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=95.8847736625514, Blast_Score=967, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=386, Percent_Identity=26.6839378238342, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54903; Mature: 54772

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFYTVVSWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERARAMWPSTAKWLNDLKSCKHIFAEENSSVASSLFKLCERRQGIAGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCE
LQLLTDSDDVMQALIRDIQLARHNIEMVFYIWQPGGMADKVAESLMAAARRGIHCRLMLD
EEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEC
SAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
CCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRFFKQDAGVGQWVDVMARMEGPIATAMGVIYSCDWEIETGKRILPPPPDVNIMPF
CCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHEECCEEECCCCCCCCCCCCCEEEEC
EQASGHTIHTIASGPGFPEDLIHQALLTATYSAREYLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCEEEECEEEECCCEEEEEEE
NLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDERLWLKRPLWQRVAERLFY
CCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FFSPLL
HHHCCC
>Mature Secondary Structure 
TTFYTVVSWLVILGYWLLIAGVTLRILMKRRAVPSAMAWLLIIYILPLVGIIAYLSFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCH
LHLGKRRAERARAMWPSTAKWLNDLKSCKHIFAEENSSVASSLFKLCERRQGIAGVKGNQ
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCE
LQLLTDSDDVMQALIRDIQLARHNIEMVFYIWQPGGMADKVAESLMAAARRGIHCRLMLD
EEEEECCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEC
SAGSVAFFRSPWAAMMRNAGIEVVEALKVNLMRVFLRRMDLRQHRKMIMIDNYIAYTGSM
CCCCEEEECCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRFFKQDAGVGQWVDVMARMEGPIATAMGVIYSCDWEIETGKRILPPPPDVNIMPF
CCCCCHHHHHCCCCCHHHHHHHHHCCCHHHHHHHHEECCEEECCCCCCCCCCCCCEEEEC
EQASGHTIHTIASGPGFPEDLIHQALLTATYSAREYLIMTTPYFVPSDDLLHAICTAAQR
CCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHHC
GVDVSIILPRKNDSMLVGWASRAFFTELLAAGVKIYQFEGGLLHTKSVLVDGELSLVGTV
CCEEEEEEECCCCCEEEEEHHHHHHHHHHHCCEEEEEECCCEEEECEEEECCCEEEEEEE
NLDMRSLWLNFEITLAIDDKGFGADLAAVQDDYISRSRLLDERLWLKRPLWQRVAERLFY
CCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
FFSPLL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA