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The map label for this gene is pspE [H]

Identifier: 218549006

GI number: 218549006

Start: 1702640

End: 1702990

Strand: Reverse

Name: pspE [H]

Synonym: EFER_1654

Alternate gene names: 218549006

Gene position: 1702990-1702640 (Counterclockwise)

Preceding gene: 218549007

Following gene: 218549005

Centisome position: 37.11

GC content: 44.16

Gene sequence:

>351_bases
TTGCCGTTTTTTACTCACCATAAGGATGCTGAGATGTATAAAAAAGGGTTATTTGTACTGGCTCTGGCGTTTTCTGTACC
CACTTTTGCTGCTGAACACTGGATTGATGTTCGTATTCCTGAGCAGTATCAAAAGGAACATATCGAAGGGGCGACCAACA
TTCCGCTAAAAGATGTTAAAACACGTATTGCAGAAGTCGTCCCGGATAAGAACGACACCGTAAAACTCTATTGTAATGGT
GGCCGTCAGTCGGGCCAGGCAAAACAGATTTTGATCGATATGGGCTACACTAACGCCCAGAATGCGGGGGGCATTGATCG
TATTGATATGCCGAAAGTGAAAGAACAGTAA

Upstream 100 bases:

>100_bases
GCTGGAACCATTGTTAACCCGTACAGCGAATAAAGTGGCACGTCGCTATAAGCGTTGAGTTCCGATTCGCATTATTCATT
AACAAAAAGGCGTATAGTGA

Downstream 100 bases:

>100_bases
TTTCCGCAGATCTAACAGGCGGCGTAAAATACGCCGCTCTGTCTCATCAAATCATTAAGTCAGATAATTCCCGAATCGGT
TTCTCAGTAATGCCTGCGGT

Product: thiosulfate:cyanide sulfurtransferase

Products: NA

Alternate protein names: TST; Phage shock protein E [H]

Number of amino acids: Translated: 116; Mature: 115

Protein sequence:

>116_residues
MPFFTHHKDAEMYKKGLFVLALAFSVPTFAAEHWIDVRIPEQYQKEHIEGATNIPLKDVKTRIAEVVPDKNDTVKLYCNG
GRQSGQAKQILIDMGYTNAQNAGGIDRIDMPKVKEQ

Sequences:

>Translated_116_residues
MPFFTHHKDAEMYKKGLFVLALAFSVPTFAAEHWIDVRIPEQYQKEHIEGATNIPLKDVKTRIAEVVPDKNDTVKLYCNG
GRQSGQAKQILIDMGYTNAQNAGGIDRIDMPKVKEQ
>Mature_115_residues
PFFTHHKDAEMYKKGLFVLALAFSVPTFAAEHWIDVRIPEQYQKEHIEGATNIPLKDVKTRIAEVVPDKNDTVKLYCNGG
RQSGQAKQILIDMGYTNAQNAGGIDRIDMPKVKEQ

Specific function: The phage shock protein (psp) operon (pspABCDE) may play a significant role in the competition for survival under nutrient- or energy-limited conditions. PspE catalyzes the sulfur-transfer reaction from thiosulfate to cyanide, to form sulfite and thiocyan

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Escherichia coli, GI1787567, Length=103, Percent_Identity=73.7864077669903, Blast_Score=170, Evalue=2e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014323
- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.1 [H]

Molecular weight: Translated: 13117; Mature: 12986

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPFFTHHKDAEMYKKGLFVLALAFSVPTFAAEHWIDVRIPEQYQKEHIEGATNIPLKDVK
CCCCCCCCCHHHHHHHHHHHHHHHHCCHHCHHCEEEEECCHHHHHHHCCCCCCCCHHHHH
TRIAEVVPDKNDTVKLYCNGGRQSGQAKQILIDMGYTNAQNAGGIDRIDMPKVKEQ
HHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PFFTHHKDAEMYKKGLFVLALAFSVPTFAAEHWIDVRIPEQYQKEHIEGATNIPLKDVK
CCCCCCCCHHHHHHHHHHHHHHHHCCHHCHHCEEEEECCHHHHHHHCCCCCCCCHHHHH
TRIAEVVPDKNDTVKLYCNGGRQSGQAKQILIDMGYTNAQNAGGIDRIDMPKVKEQ
HHHHHHCCCCCCEEEEEECCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1712397; 9097039; 9278503; 9868784 [H]