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The map label for this gene is yneJ [H]

Identifier: 218548903

GI number: 218548903

Start: 1580598

End: 1581464

Strand: Reverse

Name: yneJ [H]

Synonym: EFER_1548

Alternate gene names: 218548903

Gene position: 1581464-1580598 (Counterclockwise)

Preceding gene: 218548910

Following gene: 218548902

Centisome position: 34.46

GC content: 51.79

Gene sequence:

>867_bases
ATGGATCTTACCCAACTGGAGATGTTTAACGCGGTTGCAGAAACGGGCAGCATTACCCAGGCTGCGGCAAAGGTGCATCG
TGTTCCGTCCAATCTCACTACTCGTATCCGCCAGCTGGAAGCTGATCTGGGCGTTGAGCTGTTTATTCGTGAGAATCAGC
GGCTGCGACTTTCGCCTGCCGGACATAATTTTTTGCGCTACAGCCAACAAATTTTGGCGCTGGTGGATGAGGCCAGAATG
GTGGTTGCGGGCGATGAACCGCAAGGTCTGTTTTCATTAGGCGCTCTGGAAAGTACAGCTGCCGTACGTATCCCCGCAGT
GCTGGCGCGGTTTAATCAGCGTTATCCGAAGATTCAGTTCTCACTATCAACCGGCCCTTCCGGCACCATGTTGGAAGGCG
TGCTTGAAGGCAGACTGAATGCTGCTTTTGTTGATGGACCAATTACTCATCCGGGTCTTGAGGGTATTCCGGTGTATGAG
GAAGAGATGATGATCGTGGCACCTCACGGACATGAACCGATATACCGTGCCAGCGAGGTAAATGGCTGCAATATTTACGC
TTTTCGCGCTAACTGTTCTTATCGTCGCCATTTTGAAAGCTGGTTTCATGCTGATAACGCCATGCCCGGTACAATTCACG
AAATGGAGTCGTATCACGGGATGTTAGCCTGTGCCATTGCAGGTGCGGGTATTGCGTTAATTCCTCGTACTATGCTGGAA
AGTATGCCAGGGCATCATCAGGTAGAAGCCTGGCCATTGGCTGAAAACTGGCGCTGGCTAACCACATGGCTGGTCTGGCG
TCGCGGCGCGAAGACACGACAGCTGGAGGCGTTTATCGCGTTACTGAATGCTGAAAAGAGAGATTAA

Upstream 100 bases:

>100_bases
CATTGTATTTCCCCCATGATGTATCGATTTATATTCGCTGCATTTACTCTTTCTGAAAAATGAATAATATTGACAACATT
ATTCTTAAATTGAGAACGCC

Downstream 100 bases:

>100_bases
TGCGATCTTCATCACAAATTTATTGTCTTTCGCCTTGCTATCGCTTTAAGATCGTCGCCTCACCTTTTCGCTACTAATCT
TATACATTCTTATGACAACG

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MDLTQLEMFNAVAETGSITQAAAKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPAGHNFLRYSQQILALVDEARM
VVAGDEPQGLFSLGALESTAAVRIPAVLARFNQRYPKIQFSLSTGPSGTMLEGVLEGRLNAAFVDGPITHPGLEGIPVYE
EEMMIVAPHGHEPIYRASEVNGCNIYAFRANCSYRRHFESWFHADNAMPGTIHEMESYHGMLACAIAGAGIALIPRTMLE
SMPGHHQVEAWPLAENWRWLTTWLVWRRGAKTRQLEAFIALLNAEKRD

Sequences:

>Translated_288_residues
MDLTQLEMFNAVAETGSITQAAAKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPAGHNFLRYSQQILALVDEARM
VVAGDEPQGLFSLGALESTAAVRIPAVLARFNQRYPKIQFSLSTGPSGTMLEGVLEGRLNAAFVDGPITHPGLEGIPVYE
EEMMIVAPHGHEPIYRASEVNGCNIYAFRANCSYRRHFESWFHADNAMPGTIHEMESYHGMLACAIAGAGIALIPRTMLE
SMPGHHQVEAWPLAENWRWLTTWLVWRRGAKTRQLEAFIALLNAEKRD
>Mature_288_residues
MDLTQLEMFNAVAETGSITQAAAKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPAGHNFLRYSQQILALVDEARM
VVAGDEPQGLFSLGALESTAAVRIPAVLARFNQRYPKIQFSLSTGPSGTMLEGVLEGRLNAAFVDGPITHPGLEGIPVYE
EEMMIVAPHGHEPIYRASEVNGCNIYAFRANCSYRRHFESWFHADNAMPGTIHEMESYHGMLACAIAGAGIALIPRTMLE
SMPGHHQVEAWPLAENWRWLTTWLVWRRGAKTRQLEAFIALLNAEKRD

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787806, Length=283, Percent_Identity=87.2791519434629, Blast_Score=522, Evalue=1e-149,
Organism=Escherichia coli, GI1788887, Length=292, Percent_Identity=26.027397260274, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI1790208, Length=251, Percent_Identity=27.0916334661355, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082132, Length=264, Percent_Identity=23.4848484848485, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788481, Length=167, Percent_Identity=29.940119760479, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI157672245, Length=285, Percent_Identity=25.9649122807018, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=285, Percent_Identity=22.8070175438596, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI145693105, Length=288, Percent_Identity=21.875, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787879, Length=282, Percent_Identity=25.177304964539, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786401, Length=165, Percent_Identity=26.6666666666667, Blast_Score=65, Evalue=4e-12,
Organism=Escherichia coli, GI1787601, Length=286, Percent_Identity=22.3776223776224, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1788297, Length=257, Percent_Identity=22.9571984435798, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788626, Length=132, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789204, Length=249, Percent_Identity=24.4979919678715, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32174; Mature: 32174

Theoretical pI: Translated: 6.59; Mature: 6.59

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLTQLEMFNAVAETGSITQAAAKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARMVVAGDEPQGLFSLGALESTAAVRIPAVLARFNQRYPKIQF
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
SLSTGPSGTMLEGVLEGRLNAAFVDGPITHPGLEGIPVYEEEMMIVAPHGHEPIYRASEV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCCEEEECCCCCCCCEECCCC
NGCNIYAFRANCSYRRHFESWFHADNAMPGTIHEMESYHGMLACAIAGAGIALIPRTMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAENWRWLTTWLVWRRGAKTRQLEAFIALLNAEKRD
HCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDLTQLEMFNAVAETGSITQAAAKVHRVPSNLTTRIRQLEADLGVELFIRENQRLRLSPA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEECCCEEEECCC
GHNFLRYSQQILALVDEARMVVAGDEPQGLFSLGALESTAAVRIPAVLARFNQRYPKIQF
CCHHHHHHHHHHHHHHHCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHCCCCEEEE
SLSTGPSGTMLEGVLEGRLNAAFVDGPITHPGLEGIPVYEEEMMIVAPHGHEPIYRASEV
EECCCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCCCEECCCEEEECCCCCCCCEECCCC
NGCNIYAFRANCSYRRHFESWFHADNAMPGTIHEMESYHGMLACAIAGAGIALIPRTMLE
CCCEEEEEECCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHEEEHHHCCCHHHHHHHHHH
SMPGHHQVEAWPLAENWRWLTTWLVWRRGAKTRQLEAFIALLNAEKRD
HCCCCCCEEEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]