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The map label for this gene is mdlA [H]

Identifier: 218548830

GI number: 218548830

Start: 1510509

End: 1511717

Strand: Reverse

Name: mdlA [H]

Synonym: EFER_1473

Alternate gene names: 218548830

Gene position: 1511717-1510509 (Counterclockwise)

Preceding gene: 218548833

Following gene: 218548829

Centisome position: 32.94

GC content: 47.89

Gene sequence:

>1209_bases
ATGGAGATAATCATGTCCTTAAGTGCCAATTCTGAAGCGGTCAGTTACGCCAAAGTTGCAAACGGCAAAACTGCTGCAGA
AACCGGTGATCGTATTGAGTGGGTAAAATTGTCACTGGCTTTTTTGCCGTTAGCTACGCCTGTCAGCGACGCCAAAGTCT
TAACAGGCAGACAAAAGCCATTAACGGAAGTGGCGATTATCATTGCGGAAATTCGATCTCGTGATGGTTTTGAAGGAGTA
GGCTTCAGCTATTCAAAACGCGCTGGTGGCCAGGGGATTTATGCACATGCAAAGGAGATTGCCGATAATCTGCTTGGGGA
AGATCCGAATGATATCGACAAGATTTATAGCAAATTGCTGTGGGCTGGAGCCTCTGTTGGACGCAGTGGAATGGCTGTAC
AGGCCATCTCCCCTATTGATATCGCCTTGTGGGATATGAAAGCTAAAAGAGCCGGGTTGCCATTAGCAAAATTACTGGGT
TCACATCGGGATTCAGTGCAGTGCTACAACACATCCGGTGGTTTTCTGCATACTCCGCTCGATCAGGTATTAAAAAATGT
TGTTATCTCGCGTGAAAACGGAATTGGCGGTATCAAATTAAAAGTTGGACAGCCTGACTGTGCGGAAGATATTCGTCGAC
TTACTGCGGTACGTGAAGCACTGGGGGATGATTTCCCGCTAATGGTAGATGCCAATCAACAATGGGATCGGGAAACAGCT
ATCAGGATGGGGCGAAAAATGGAACACTTTAATCTCATCTGGATTGAAGAGCCGCTGGATGCCTATGACATAGAGGGGCA
CGCACAGCTAGCAGCTGCGCTGGATACCCCTATAGCAACCGGAGAGATGCTGACCAGTTTCCGCGAGCATGAGCAATTGA
TTCTTGGAAATGCCAGCGATTTTGTGCAGCCAGATGCTCCACGCGTGGGCGGTATCTCACCATTTTTAAAAATCATGGAT
CTGGCGGCGAAACATGGTCGCAAACTGGCACCGCATTTTGCAATGGAGGTTCATTTGCATCTTGCTGCAGCTTACCCACT
GGAACCGTGGCTGGAACATTTTGAATGGTTAAATCCGCTATTTAACGAACAACTGGAACTGCGCGATGGACGGATGTGGG
TTTCTGAACGTCATGGTTTAGGTTTTACGCTGAGTGAACAGGCACGTCGTTGGACACAGTTAAGCTGTGAATTCGGGAAA
CAGCCGTAA

Upstream 100 bases:

>100_bases
ATTCGGTCTTAAGTTGTGAGCTATTGTTCAATTATAACTCTTTCAACTGGAATGGTTATTTCATAGAGATTACTAATGTT
AGCGCAAACATAATTGATAA

Downstream 100 bases:

>100_bases
ACAGGCAATAAATACTGAATTACAGTTCTACCCTGCTGATACCAGTGAAATGCTTAATTATTTTCAAAATACTGTTCCGG
GAATACATCCGGAGCAGCAT

Product: mandelate racemase

Products: NA

Alternate protein names: MR [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MEIIMSLSANSEAVSYAKVANGKTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEVAIIIAEIRSRDGFEGV
GFSYSKRAGGQGIYAHAKEIADNLLGEDPNDIDKIYSKLLWAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLG
SHRDSVQCYNTSGGFLHTPLDQVLKNVVISRENGIGGIKLKVGQPDCAEDIRRLTAVREALGDDFPLMVDANQQWDRETA
IRMGRKMEHFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQPDAPRVGGISPFLKIMD
LAAKHGRKLAPHFAMEVHLHLAAAYPLEPWLEHFEWLNPLFNEQLELRDGRMWVSERHGLGFTLSEQARRWTQLSCEFGK
QP

Sequences:

>Translated_402_residues
MEIIMSLSANSEAVSYAKVANGKTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEVAIIIAEIRSRDGFEGV
GFSYSKRAGGQGIYAHAKEIADNLLGEDPNDIDKIYSKLLWAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLG
SHRDSVQCYNTSGGFLHTPLDQVLKNVVISRENGIGGIKLKVGQPDCAEDIRRLTAVREALGDDFPLMVDANQQWDRETA
IRMGRKMEHFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQPDAPRVGGISPFLKIMD
LAAKHGRKLAPHFAMEVHLHLAAAYPLEPWLEHFEWLNPLFNEQLELRDGRMWVSERHGLGFTLSEQARRWTQLSCEFGK
QP
>Mature_402_residues
MEIIMSLSANSEAVSYAKVANGKTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKPLTEVAIIIAEIRSRDGFEGV
GFSYSKRAGGQGIYAHAKEIADNLLGEDPNDIDKIYSKLLWAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLG
SHRDSVQCYNTSGGFLHTPLDQVLKNVVISRENGIGGIKLKVGQPDCAEDIRRLTAVREALGDDFPLMVDANQQWDRETA
IRMGRKMEHFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASDFVQPDAPRVGGISPFLKIMD
LAAKHGRKLAPHFAMEVHLHLAAAYPLEPWLEHFEWLNPLFNEQLELRDGRMWVSERHGLGFTLSEQARRWTQLSCEFGK
QP

Specific function: Probably Involved In The Degradation Of Homoserine Lactone (Hsl) Or Of A Metabolite Of Hsl That Signals Starvation. [C]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544119, Length=328, Percent_Identity=27.1341463414634, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI42544117, Length=325, Percent_Identity=25.5384615384615, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI186972148, Length=167, Percent_Identity=34.1317365269461, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI1787864, Length=368, Percent_Identity=24.4565217391304, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI226510960, Length=324, Percent_Identity=27.4691358024691, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994953, Length=263, Percent_Identity=28.8973384030418, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR015654
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =5.1.2.2 [H]

Molecular weight: Translated: 44426; Mature: 44426

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIMSLSANSEAVSYAKVANGKTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKP
CCEEEEECCCCCHHHHHHHCCCCCHHCCCCCEEEEEEEEHHHCCCCCCCCCHHHCCCCCC
LTEVAIIIAEIRSRDGFEGVGFSYSKRAGGQGIYAHAKEIADNLLGEDPNDIDKIYSKLL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
WAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGSHRDSVQCYNTSGGFLHTPL
HHCCCCCCCCCEEEECCCCEEEEECCHHHHCCCCHHHHHCCCCCCEEEECCCCCEEECCH
DQVLKNVVISRENGIGGIKLKVGQPDCAEDIRRLTAVREALGDDFPLMVDANQQWDRETA
HHHHHHHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
IRMGRKMEHFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASD
HHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCHH
FVQPDAPRVGGISPFLKIMDLAAKHGRKLAPHFAMEVHLHLAAAYPLEPWLEHFEWLNPL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEEECCCHHHHHHHHHHHHHH
FNEQLELRDGRMWVSERHGLGFTLSEQARRWTQLSCEFGKQP
HCCCEECCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEIIMSLSANSEAVSYAKVANGKTAAETGDRIEWVKLSLAFLPLATPVSDAKVLTGRQKP
CCEEEEECCCCCHHHHHHHCCCCCHHCCCCCEEEEEEEEHHHCCCCCCCCCHHHCCCCCC
LTEVAIIIAEIRSRDGFEGVGFSYSKRAGGQGIYAHAKEIADNLLGEDPNDIDKIYSKLL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHH
WAGASVGRSGMAVQAISPIDIALWDMKAKRAGLPLAKLLGSHRDSVQCYNTSGGFLHTPL
HHCCCCCCCCCEEEECCCCEEEEECCHHHHCCCCHHHHHCCCCCCEEEECCCCCEEECCH
DQVLKNVVISRENGIGGIKLKVGQPDCAEDIRRLTAVREALGDDFPLMVDANQQWDRETA
HHHHHHHHEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHH
IRMGRKMEHFNLIWIEEPLDAYDIEGHAQLAAALDTPIATGEMLTSFREHEQLILGNASD
HHHCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHEEECCCHH
FVQPDAPRVGGISPFLKIMDLAAKHGRKLAPHFAMEVHLHLAAAYPLEPWLEHFEWLNPL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHEEEEEEEEECCCHHHHHHHHHHHHHH
FNEQLELRDGRMWVSERHGLGFTLSEQARRWTQLSCEFGKQP
HCCCEECCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2831968; 2271624; 2215699; 1909893; 1892834; 7893689; 7893690 [H]