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The map label for this gene is ynfL [H]

Identifier: 218548801

GI number: 218548801

Start: 1483290

End: 1484189

Strand: Direct

Name: ynfL [H]

Synonym: EFER_1440

Alternate gene names: 218548801

Gene position: 1483290-1484189 (Clockwise)

Preceding gene: 218548797

Following gene: 218548802

Centisome position: 32.32

GC content: 50.56

Gene sequence:

>900_bases
ATGAATATAGAATTGCGTCATCTCCGCTATTTTGTCGCAGTGGCAGAGGAACTCCATTTTGGGCGAGCTGCGGCGCGGCT
TAACATTTCTCAGCCACCACTTAGCCAGCAAATCCAGATTCTGGAACAGCAAATTGGTGCGCGATTACTGGCACGAACAA
ACCGAAGTGTGGCGCTCACTGCGGCAGGAAGGCAGTTTTTGGCAGACTGTCGCCAAATCCTTGGACTGGTGAATGATGCC
GCTGCAAGGGCAGAGCGTCTGCATCAGGGCGAAGAAGGGGAGCTACGTATTGGCTTTACCTCGTCGGCGCCATTTATAAA
AGCTGTATCGGACACCTTGTCGTTATTTCGTCACGACTATCCTGAAGTGCATTTACAAACCAGGGAAATGAATACACGCG
AACAAATTGCGCCGCTTAACGAAGGTACGCTCGACCTCGGTTTGCTGCGCAATACACCGTTACCGGACACGCTGAATTAT
GCCGTCATCCGCCACGAACCGTTGATGGCCCTCGTTCCTCAAGCTCATGCTCTGGCGAAAAAAAAGAGAGTTACGCTGGC
TGAACTGGTTAAAGAACCCTTTGTTTTTTTTGATCCGCAGGTAGGTACTGGCCTGTATGACGACATTCTTGGTCTAATGC
GTCGCTATAACCTGACGCCATTGATTACTCAGGAAGTTGGTGAAGCAATGACCATTATTGGGCTGGTAGCAGCAGGGTTA
GGCGTTTCTATTCTTCCTGCGTCTTTCGAGCGGGTACAACTGGAGGAAATGTGTTGGTTACCCATCGCAGAAGAGGACGC
AGTATCTGAAATGTGGCTGGTTTGGCCAAAACATCGGGAACAGAGTCAAGCTGCCGTGCGCTTTCGTGATCAGCTGCTGA
ACGCAGCGAGATGCACATAA

Upstream 100 bases:

>100_bases
CCCCGTAATTATCGGTAACGGGTATGACCACACCCTAAGCGTAGAAAAATCCGATTATTATGTCTAATATATTAATAATC
TTAAATAAGACGTTTTAAAT

Downstream 100 bases:

>100_bases
TTTGGCTCAACAGTCATGAAAAATGTGCGTCAAATCACACGGCTAAGTAAAATTTTTCCGTTACGTGTTGAAGTGCTTCA
CCATAACCGACAAATTATTT

Product: DNA-binding transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQIGARLLARTNRSVALTAAGRQFLADCRQILGLVNDA
AARAERLHQGEEGELRIGFTSSAPFIKAVSDTLSLFRHDYPEVHLQTREMNTREQIAPLNEGTLDLGLLRNTPLPDTLNY
AVIRHEPLMALVPQAHALAKKKRVTLAELVKEPFVFFDPQVGTGLYDDILGLMRRYNLTPLITQEVGEAMTIIGLVAAGL
GVSILPASFERVQLEEMCWLPIAEEDAVSEMWLVWPKHREQSQAAVRFRDQLLNAARCT

Sequences:

>Translated_299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQIGARLLARTNRSVALTAAGRQFLADCRQILGLVNDA
AARAERLHQGEEGELRIGFTSSAPFIKAVSDTLSLFRHDYPEVHLQTREMNTREQIAPLNEGTLDLGLLRNTPLPDTLNY
AVIRHEPLMALVPQAHALAKKKRVTLAELVKEPFVFFDPQVGTGLYDDILGLMRRYNLTPLITQEVGEAMTIIGLVAAGL
GVSILPASFERVQLEEMCWLPIAEEDAVSEMWLVWPKHREQSQAAVRFRDQLLNAARCT
>Mature_299_residues
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQIGARLLARTNRSVALTAAGRQFLADCRQILGLVNDA
AARAERLHQGEEGELRIGFTSSAPFIKAVSDTLSLFRHDYPEVHLQTREMNTREQIAPLNEGTLDLGLLRNTPLPDTLNY
AVIRHEPLMALVPQAHALAKKKRVTLAELVKEPFVFFDPQVGTGLYDDILGLMRRYNLTPLITQEVGEAMTIIGLVAAGL
GVSILPASFERVQLEEMCWLPIAEEDAVSEMWLVWPKHREQSQAAVRFRDQLLNAARCT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=297, Percent_Identity=85.1851851851852, Blast_Score=513, Evalue=1e-147,
Organism=Escherichia coli, GI1788887, Length=275, Percent_Identity=36.3636363636364, Blast_Score=157, Evalue=8e-40,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=34.7328244274809, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1788748, Length=249, Percent_Identity=29.718875502008, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1790399, Length=248, Percent_Identity=29.4354838709677, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI145693105, Length=295, Percent_Identity=26.1016949152542, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081904, Length=195, Percent_Identity=34.3589743589744, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI157672245, Length=249, Percent_Identity=31.3253012048193, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1788297, Length=274, Percent_Identity=28.4671532846715, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790208, Length=248, Percent_Identity=27.0161290322581, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790262, Length=254, Percent_Identity=26.7716535433071, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787530, Length=269, Percent_Identity=25.6505576208178, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789506, Length=286, Percent_Identity=23.4265734265734, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1787589, Length=123, Percent_Identity=36.5853658536585, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786713, Length=246, Percent_Identity=27.6422764227642, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787601, Length=149, Percent_Identity=32.8859060402685, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33530; Mature: 33530

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQIGARLLARTNRSVALT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRQFLADCRQILGLVNDAAARAERLHQGEEGELRIGFTSSAPFIKAVSDTLSLFRHDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PEVHLQTREMNTREQIAPLNEGTLDLGLLRNTPLPDTLNYAVIRHEPLMALVPQAHALAK
CCEEEECCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KKRVTLAELVKEPFVFFDPQVGTGLYDDILGLMRRYNLTPLITQEVGEAMTIIGLVAAGL
HHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GVSILPASFERVQLEEMCWLPIAEEDAVSEMWLVWPKHREQSQAAVRFRDQLLNAARCT
CCEECCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQILEQQIGARLLARTNRSVALT
CCCCHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEE
AAGRQFLADCRQILGLVNDAAARAERLHQGEEGELRIGFTSSAPFIKAVSDTLSLFRHDY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCC
PEVHLQTREMNTREQIAPLNEGTLDLGLLRNTPLPDTLNYAVIRHEPLMALVPQAHALAK
CCEEEECCCCCCHHHHCCCCCCCEEEEECCCCCCCCCCCEEEEECCCHHHHHHHHHHHHH
KKRVTLAELVKEPFVFFDPQVGTGLYDDILGLMRRYNLTPLITQEVGEAMTIIGLVAAGL
HHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
GVSILPASFERVQLEEMCWLPIAEEDAVSEMWLVWPKHREQSQAAVRFRDQLLNAARCT
CCEECCCCHHHHHHHHHHCCCCCCHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]