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The map label for this gene is rrmA [H]

Identifier: 218548619

GI number: 218548619

Start: 1291467

End: 1292276

Strand: Direct

Name: rrmA [H]

Synonym: EFER_1253

Alternate gene names: 218548619

Gene position: 1291467-1292276 (Clockwise)

Preceding gene: 218548618

Following gene: 218548626

Centisome position: 28.14

GC content: 47.04

Gene sequence:

>810_bases
ATGTCGTTTTCCTGTCCATTATGTCATCAGTCTCTTACTCAAACTCAAACCAGCTATGTTTGCCCACTGCGACACCAGTT
TGATGTAGCGAAAGAGGGATATGTGAATCTGCTGCCTGTACAACATAAGCGTTCTCGTGATCCTGGCGATAGTGCGGAAA
TGATGCAGGCTCGACGGGCTTTTCTCGATGCCGGACATTATCTTCCTTTGCGTGAAGCGATTGTCGCCATCCTGACTGAA
CAACTGAGCAAAAACGCCAGTGCAATTCTGGATATTGGTTGTGGGGAAGGGTATTACACGCACGCATTTGCAGATGCGGT
TCCCGCAGTTACGACTTATGGGTTGGATGTCTCTAAGGTCGCTATCAGAGCTGCAGCGAAGCGTTATTCACAAGTCAAAT
TTTGCGTGGCCTCCAGTCATCGGTTACCGTTTGCTGATGCCTGTATGGACGCAATTATTCGCATTTATGCACCCTGCAAA
GCTGAAGAGTTAGCGCGAGTGGTAAAACCCGGTGGATTAGTGATCACTGCAACGCCTGGTCCACACCACTTAATGGAACT
GAAAGGACTTATTTATGAACAGGTACGGTTACACGATCCACATACAGAAGTACTGGAAGGTTTCAATTTGGTGAAGGGGA
TTTCATTAAGCTACCCAATGCATTTGAAAGGCAGTGAAGCGGTTGCGTTACTTCAGATGACACCATTTGCGTGGCGCGCG
AAGACAGAAGTATGGGAAGAGTTAGCTACCAAAGCTATATTTGACTGCCAGACGGATTTTAATCTTCACATCTGGCAGCG
GTCGAATTAA

Upstream 100 bases:

>100_bases
CAGTGCGAGGCTTTTTAACATCTGTATCCAGTGGGTTGTTGTTTGCCGGACCCGTTAATCGCGCTAAACTTGCGGCCTTT
AGTATACTGCCGGAGTAGCC

Downstream 100 bases:

>100_bases
CCGTGGAAATGCGTCCAGAGGATCTGGACGCCGATACCTATCAGTACCAGCCCACCCAGAATTTCAGCTTTTTTGCCAAG
AATCGGTCCGATAAAGCGTC

Product: Ribosomal RNA large subunit methyltransferase A (rRNA (guanine-N(1)-)-methyltransferase) (23S rRNA m1G745 methyltransferase)

Products: NA

Alternate protein names: 23S rRNA m1G745 methyltransferase; rRNA (guanine-N(1)-)-methyltransferase [H]

Number of amino acids: Translated: 269; Mature: 268

Protein sequence:

>269_residues
MSFSCPLCHQSLTQTQTSYVCPLRHQFDVAKEGYVNLLPVQHKRSRDPGDSAEMMQARRAFLDAGHYLPLREAIVAILTE
QLSKNASAILDIGCGEGYYTHAFADAVPAVTTYGLDVSKVAIRAAAKRYSQVKFCVASSHRLPFADACMDAIIRIYAPCK
AEELARVVKPGGLVITATPGPHHLMELKGLIYEQVRLHDPHTEVLEGFNLVKGISLSYPMHLKGSEAVALLQMTPFAWRA
KTEVWEELATKAIFDCQTDFNLHIWQRSN

Sequences:

>Translated_269_residues
MSFSCPLCHQSLTQTQTSYVCPLRHQFDVAKEGYVNLLPVQHKRSRDPGDSAEMMQARRAFLDAGHYLPLREAIVAILTE
QLSKNASAILDIGCGEGYYTHAFADAVPAVTTYGLDVSKVAIRAAAKRYSQVKFCVASSHRLPFADACMDAIIRIYAPCK
AEELARVVKPGGLVITATPGPHHLMELKGLIYEQVRLHDPHTEVLEGFNLVKGISLSYPMHLKGSEAVALLQMTPFAWRA
KTEVWEELATKAIFDCQTDFNLHIWQRSN
>Mature_268_residues
SFSCPLCHQSLTQTQTSYVCPLRHQFDVAKEGYVNLLPVQHKRSRDPGDSAEMMQARRAFLDAGHYLPLREAIVAILTEQ
LSKNASAILDIGCGEGYYTHAFADAVPAVTTYGLDVSKVAIRAAAKRYSQVKFCVASSHRLPFADACMDAIIRIYAPCKA
EELARVVKPGGLVITATPGPHHLMELKGLIYEQVRLHDPHTEVLEGFNLVKGISLSYPMHLKGSEAVALLQMTPFAWRAK
TEVWEELATKAIFDCQTDFNLHIWQRSN

Specific function: Specifically methylates the guanosine in position 745 of 23S rRNA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmA family [H]

Homologues:

Organism=Escherichia coli, GI1788125, Length=268, Percent_Identity=79.4776119402985, Blast_Score=458, Evalue=1e-130,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013216
- InterPro:   IPR016718 [H]

Pfam domain/function: PF08241 Methyltransf_11 [H]

EC number: =2.1.1.187 [H]

Molecular weight: Translated: 29929; Mature: 29798

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSCPLCHQSLTQTQTSYVCPLRHQFDVAKEGYVNLLPVQHKRSRDPGDSAEMMQARRA
CCCCCCHHHHHHHHHHHHEECCCHHHHCCCCCCCEEEEECCHHCCCCCCCHHHHHHHHHH
FLDAGHYLPLREAIVAILTEQLSKNASAILDIGCGEGYYTHAFADAVPAVTTYGLDVSKV
HHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHH
AIRAAAKRYSQVKFCVASSHRLPFADACMDAIIRIYAPCKAEELARVVKPGGLVITATPG
HHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCC
PHHLMELKGLIYEQVRLHDPHTEVLEGFNLVKGISLSYPMHLKGSEAVALLQMTPFAWRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEECCCHHHH
KTEVWEELATKAIFDCQTDFNLHIWQRSN
HHHHHHHHHHHHHHCCCCCCEEEEEECCC
>Mature Secondary Structure 
SFSCPLCHQSLTQTQTSYVCPLRHQFDVAKEGYVNLLPVQHKRSRDPGDSAEMMQARRA
CCCCCHHHHHHHHHHHHEECCCHHHHCCCCCCCEEEEECCHHCCCCCCCHHHHHHHHHH
FLDAGHYLPLREAIVAILTEQLSKNASAILDIGCGEGYYTHAFADAVPAVTTYGLDVSKV
HHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHH
AIRAAAKRYSQVKFCVASSHRLPFADACMDAIIRIYAPCKAEELARVVKPGGLVITATPG
HHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCC
PHHLMELKGLIYEQVRLHDPHTEVLEGFNLVKGISLSYPMHLKGSEAVALLQMTPFAWRA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEEECCCHHHH
KTEVWEELATKAIFDCQTDFNLHIWQRSN
HHHHHHHHHHHHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7984109; 9097040; 9278503; 9440525 [H]