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The map label for this gene is poxB [H]

Identifier: 218548390

GI number: 218548390

Start: 1041449

End: 1043167

Strand: Reverse

Name: poxB [H]

Synonym: EFER_1014

Alternate gene names: 218548390

Gene position: 1043167-1041449 (Counterclockwise)

Preceding gene: 218548392

Following gene: 218548389

Centisome position: 22.73

GC content: 53.05

Gene sequence:

>1719_bases
ATGAAACAAACGGTTGCAGCTTATATCGCCAAAACACTCGAATCAGCAGGGGTAAAACGCATTTGGGGAGTGACGGGCGA
CTCTCTGAACGGTCTTAGCGACAGTCTTAATCGCATGGGCACCATCGAGTGGATGTCCACCCGTCATGAAGAAGTGGCGG
CCTTTGCCGCTGGCGCTGAAGCCCAACTTAGCGGGGAACTGGCGGTCTGCGCCGGATCATGCGGACCTGGCAACCTGCAC
TTAATCAACGGTTTGTTCGATTGTCACCGTAATCACGTTCCGGTACTGGCAATTGCCGCTCATATCCCTTCCAGCGAAAT
TGGCAGCGGCTATTTCCAGGAAACCCACCCGCAAGAGTTATTCCGCGAATGTAGCCATTATTGCGAGCTGGTTTCCAGCC
CGGAGCAGATCCCACAAGTGCTGGCGATTGCTATGCGTAAAGCGGTGCTAAACCGTGGCGTTTCGGTTGTCGTTTTACCG
GGCGACGTGGCGCTAAAACCTGCACCGGAAGGGGCAACTACCCACTGGTATCACGCCCCCTTACCCGTTGTCACACCTGA
AGAAGAAGAGTTACGCAAACTGGCGCAACTGCTGCGTTACTCAAGCAATATCGCCCTGATGTGCGGTAGCGGCTGCGCGG
GGGCGCATAAAGAGCTCGTCGAGTTTGCCGGAAAAATTAAAGCGCCAATTGTTCATGCCCTGCGCGGTAAAGAGCATGTC
GAATACGATAATCCGTATGATGTCGGAATGACGGGATTAATCGGCTTCTCGTCAGGTTTTCATACCATGATGAACGCCGA
CACGTTAGTGCTGCTCGGCACCCAATTCCCCTACCGTGCGTTCTACCCGACCGATGCCAAAATTATTCAGATTGATATCA
ACCCGGCCAGCATCGGCGCGCACAGTAAGGTGGATATGGCACTGGTCGGTGATATCAAATCGACTCTGCGTGCATTGCTG
CCTTTAGTGGAAGAAAAAACCGATCGCAAATTCCTTGATAAAGCGCTGGAAAATTACCGCGATGCCCGTAAAGGGCTGGA
CGATTTAGCTAAACCGAGTGAGAAAGCCATTCACCCGCAATATCTGGCGCAGCAAATTAGCCATTTTGCTGCCGATGACG
CCATTTTCACCTGTGATGTCGGCACACCAACGGTATGGGCGGCGCGTTATCTGAAAATGAACGGCAAGCGCCGCCTGTTA
GGTTCTTTTAACCACGGTTCGATGGCTAATGCCATGCCTCAGGCGCTGGGCGCGCAGGCAACCGAGCCGGAACGTCAGGT
GGTCGCCATGTGCGGTGATGGCGGTTTCAGTATGTTGATGGGCGATTTTCTCTCAGTGGTACAAATGAAGTTGCCGGTAA
AAATTATCGTCTTTAACAACAGTGTGCTGGGCTTTGTGGCGATGGAGATGAAAGCTGGTGGCTACCTGACTGACGGCACC
GAACTGCATGACACCAACTTTGCCCGCATTGCTGAAGCGTGCGGCATTATGGGTATTCGCGTGGAAAAAGCGTCTGAAGT
CGATGAAGCCCTGCAACGCGCATTCTCCATCGACGGCCCGGTACTGGTAGATGTGGTGGTTGCGAAAGAAGAATTAGCCA
TTCCGCCGCAGATCAAACTCGAACAAGCCAAAGGTTTTAGCCTGTATATGCTGCGCGCAATCATCAGCGGACGCGGCGAT
GAAGTGATCGAACTGGCGAAAACGAACTGGCTAAGGTAA

Upstream 100 bases:

>100_bases
TTACCGGATTAAAAGCAACTGAAATATCCAGATTTGGCCAGCGTAACAGGCAGCATCTACTAAACTTGTTACCGTTATCA
CATTCAGGAGATGGAGAACC

Downstream 100 bases:

>100_bases
AAAGGGTGGCATTTCCCGTCATAATAAGGACATGCCATGATTGATTTACGCAGTGATACCGTTACCCGACCGAGCCGCGC
CATGCTCGAAGCAATGATGG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 572; Mature: 572

Protein sequence:

>572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPLPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKTDRKFLDKALENYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGIMGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Sequences:

>Translated_572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPLPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKTDRKFLDKALENYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGIMGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR
>Mature_572_residues
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
GDVALKPAPEGATTHWYHAPLPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALL
PLVEEKTDRKFLDKALENYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLL
GSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
ELHDTNFARIAEACGIMGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGD
EVIELAKTNWLR

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=559, Percent_Identity=23.7924865831843, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI21361361, Length=586, Percent_Identity=23.5494880546075, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1787096, Length=572, Percent_Identity=98.9510489510489, Blast_Score=1172, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=30.1785714285714, Blast_Score=219, Evalue=4e-58,
Organism=Escherichia coli, GI1790104, Length=545, Percent_Identity=28.9908256880734, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1786717, Length=548, Percent_Identity=25, Blast_Score=151, Evalue=1e-37,
Organism=Escherichia coli, GI1788716, Length=540, Percent_Identity=22.962962962963, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17531299, Length=559, Percent_Identity=26.4758497316637, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17531301, Length=565, Percent_Identity=27.2566371681416, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17542570, Length=583, Percent_Identity=21.9554030874786, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=555, Percent_Identity=25.4054054054054, Blast_Score=176, Evalue=8e-45,
Organism=Saccharomyces cerevisiae, GI6320816, Length=542, Percent_Identity=23.2472324723247, Blast_Score=103, Evalue=8e-23,
Organism=Drosophila melanogaster, GI19922626, Length=473, Percent_Identity=25.369978858351, Blast_Score=124, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62040; Mature: 62040

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHCCCC
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCEEECC
LPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEECCHHCCCC
HSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDKALENYRDARKGLDDLAKPSEKAIHPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGIMGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEWMSTRHEEVAAFAAGAE
CCHHHHHHHHHHHHHCCCCEEECCCCCCCCCHHHHHHHCEEHHHHHHHHHHHHHHHCCCC
AQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEIGSGYFQETHPQEL
CEECCCEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCHHCCCCCCCCCCHHHH
FRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLPGDVALKPAPEGATTHWYHAP
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEEECCCCCCCCCCEEECC
LPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKELVEFAGKIKAPIVHALRGKEHV
CCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQFPYRAFYPTDAKIIQIDINPASIGA
CCCCCCCCCCCEEEECCCCCHHHHCCCEEEEEECCCCCEEECCCCCEEEEEECCHHCCCC
HSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDKALENYRDARKGLDDLAKPSEKAIHPQ
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCCCHH
YLAQQISHFAADDAIFTCDVGTPTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQA
HHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCC
TEPERQVVAMCGDGGFSMLMGDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGT
CCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCEEEEEECCCEEEEEEEEECCCCEECCCC
ELHDTNFARIAEACGIMGIRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKL
CCCCCHHHHHHHHHCEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEEEHHHHCCCCCCCH
EQAKGFSLYMLRAIISGRGDEVIELAKTNWLR
HCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]