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The map label for this gene is ybjI [H]

Identifier: 218548363

GI number: 218548363

Start: 1017454

End: 1018269

Strand: Reverse

Name: ybjI [H]

Synonym: EFER_0985

Alternate gene names: 218548363

Gene position: 1018269-1017454 (Counterclockwise)

Preceding gene: 218548364

Following gene: 218548362

Centisome position: 22.19

GC content: 42.03

Gene sequence:

>816_bases
ATGAGCATCAAATTAATTGCGGTAGATATGGATGGTACGTTTCTCAGCGATCAAAAAACCTATAACCGCGAGCGGTTTAT
GGCTCAGTATCAACAGATGAAAGCACAAGGAATTCGCTTTGTGGTCGCCAGTGGCAATCAATATTACCAGTTGATCTCTT
TTTTCCCGGAGATTGCCAGTGAGATCTCTTTTGTGGCAGAAAACGGTGGATGGGTGGTCAGCGAAGGTGAGGATATCTTT
AATGGCGAACTGGCAAAAGACGATTTTAATACCATTGTGGATCATTTACTAACGCGTTCGGATATAGAAGTTATCGCTTG
TGGTAAAAATAGCGCTTATACCCTGAAAGAATATAACGATGAGTTAAAAACGGTGGCAGCCATGTATTATCACCGTCTGG
AATTTGTCGATAGCTTCAAAAATATCAATGATGTATTTTTCAAATTTGGTCTTAATATCTCTGATGAGCGCATCCCTGAA
GTACAAAAAGCCCTGCATGAATCTATCGGCGATATTATGGTGCCAGTACATACTGGTTACGGCAGCATTGATTTAATTAT
CCCTGGTGTTCACAAAGCTAATGGTATCCGTTTGTTACAGCAACGCTGGGGTATAAAAGATGAGGAAGTTGTTACCTTTG
GCGACGGCGGAAATGATATTGAAATGTTGCGTCATGCGGGCTACAGCTTTGCTATGTCTCACGCGGGAGAAGCTGTTGTC
GCTGCTGCGAAGTACCGTGCAGGTTCGAATAATCAGGAAGCAGTGCTGGATGTTATCGACAAAATTTTAAATCACGAAGC
GCCATTTAATCAATAA

Upstream 100 bases:

>100_bases
CCATGTTAGTGGTGCTGGCACTGGTGATATTAGCAGCACTGGTCGCCAAAGCCGTCGCTAAACCGGATACAAAAACGCAA
TCTGCTTTGGAGAATGGATA

Downstream 100 bases:

>100_bases
AACAGCAGCACGCCCGGCAACACAATGCATAAATGAACTCAGCATAATTCGCACAAACATACTCGCCGGATAATTATTCC
GGCCTTCTTTCAGGCGAATT

Product: phosphatase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIASEISFVAENGGWVVSEGEDIF
NGELAKDDFNTIVDHLLTRSDIEVIACGKNSAYTLKEYNDELKTVAAMYYHRLEFVDSFKNINDVFFKFGLNISDERIPE
VQKALHESIGDIMVPVHTGYGSIDLIIPGVHKANGIRLLQQRWGIKDEEVVTFGDGGNDIEMLRHAGYSFAMSHAGEAVV
AAAKYRAGSNNQEAVLDVIDKILNHEAPFNQ

Sequences:

>Translated_271_residues
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIASEISFVAENGGWVVSEGEDIF
NGELAKDDFNTIVDHLLTRSDIEVIACGKNSAYTLKEYNDELKTVAAMYYHRLEFVDSFKNINDVFFKFGLNISDERIPE
VQKALHESIGDIMVPVHTGYGSIDLIIPGVHKANGIRLLQQRWGIKDEEVVTFGDGGNDIEMLRHAGYSFAMSHAGEAVV
AAAKYRAGSNNQEAVLDVIDKILNHEAPFNQ
>Mature_270_residues
SIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIASEISFVAENGGWVVSEGEDIFN
GELAKDDFNTIVDHLLTRSDIEVIACGKNSAYTLKEYNDELKTVAAMYYHRLEFVDSFKNINDVFFKFGLNISDERIPEV
QKALHESIGDIMVPVHTGYGSIDLIIPGVHKANGIRLLQQRWGIKDEEVVTFGDGGNDIEMLRHAGYSFAMSHAGEAVVA
AAKYRAGSNNQEAVLDVIDKILNHEAPFNQ

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates FMN and beta-glucose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI87081790, Length=271, Percent_Identity=82.6568265682657, Blast_Score=478, Evalue=1e-136,
Organism=Escherichia coli, GI1787043, Length=270, Percent_Identity=51.8518518518518, Blast_Score=294, Evalue=5e-81,
Organism=Escherichia coli, GI2367265, Length=279, Percent_Identity=26.5232974910394, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30408; Mature: 30276

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAS
CCEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHH
EISFVAENGGWVVSEGEDIFNGELAKDDFNTIVDHLLTRSDIEVIACGKNSAYTLKEYND
HHHEEECCCCEEEECCCHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHH
ELKTVAAMYYHRLEFVDSFKNINDVFFKFGLNISDERIPEVQKALHESIGDIMVPVHTGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGIRLLQQRWGIKDEEVVTFGDGGNDIEMLRHAGYSFAMSHAGEAVV
CCEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHCCCHHH
AAAKYRAGSNNQEAVLDVIDKILNHEAPFNQ
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVASGNQYYQLISFFPEIAS
CEEEEEEECCCCEECCCHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHH
EISFVAENGGWVVSEGEDIFNGELAKDDFNTIVDHLLTRSDIEVIACGKNSAYTLKEYND
HHHEEECCCCEEEECCCHHCCCCCCCCHHHHHHHHHHCCCCCEEEEECCCCCEEHHHHHH
ELKTVAAMYYHRLEFVDSFKNINDVFFKFGLNISDERIPEVQKALHESIGDIMVPVHTGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCEEEEEECCC
GSIDLIIPGVHKANGIRLLQQRWGIKDEEVVTFGDGGNDIEMLRHAGYSFAMSHAGEAVV
CCEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCHHHHHHCCCHHH
AAAKYRAGSNNQEAVLDVIDKILNHEAPFNQ
HHHHHCCCCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]