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The map label for this gene is rimO [H]

Identifier: 218548354

GI number: 218548354

Start: 1008798

End: 1010123

Strand: Reverse

Name: rimO [H]

Synonym: EFER_0976

Alternate gene names: 218548354

Gene position: 1010123-1008798 (Counterclockwise)

Preceding gene: 218548357

Following gene: 218548347

Centisome position: 22.01

GC content: 49.77

Gene sequence:

>1326_bases
ATGAGCAAAGTAACCCATCAGCCGAAAATCGGCTTCGTTTCATTAGGCTGCCCTAAAAACCTCGTTGACTCGGAACGTAT
CCTCACTGAGCTGCGTACCGAAGGGTATGACGTTGTGCCGAGCTATGACGACGCCGATATGGTTATCGTTAACACCTGTG
GCTTTATAGACAGCGCGGTACAGGAGTCTCTGGAAGCCATTGGCGAAGCATTGAATGAAAACGGTAAAGTGATCGTTACC
GGCTGCCTTGGGGCTAAAGAAGATCAGATCCGTGAAGTCCATCCGAAAGTGCTGGAGATCACTGGTCCTCATAGCTACGA
GCAAGTTCTGGAGCATGTTCATCACTACGTGCCAAAACCGAAACACAATCCATTCCTCAGCCTGGTGCCAGAGCAAGGTG
TCAAACTAACACCACGTCATTATGCTTATCTGAAGATTTCCGAAGGCTGTAACCATCGCTGTACGTTCTGCATTATTCCG
TCTATGCGTGGCGATCTGGTAAGCCGCCCCATCGGTGATGTTTTAAGTGAAGCCAAACGTCTGGTGGATGCGGGCGTTAA
AGAGATCCTGGTGATTTCACAGGACACTTCAGCCTATGGTGTAGACGTAAAACACCGTACCGGTTTTTATAACGGCGAGC
CGGTTAAAACCAGCATGGTGAGTCTGTGTGAGCAGTTATCTAAACTGGGGATCTGGACGCGTTTACATTACGTCTATCCT
TATCCACATGTTGATGACGTGATCCCGCTGATGGCGGAAGGTAAAATTCTGCCTTACCTCGATATTCCGTTACAGCACGC
CAGTCCGCGCATTCTTAAGCTGATGAAGCGACCAGGATCCGTTGATCGTCAGCTGGCACGTATTAAACAGTGGCGCGAAA
TTTGCCCGGAACTCACACTACGTTCGACCTTTATTGTTGGCTTCCCTGGCGAAACAGAAGAAGATTTCCAGATGCTACTC
GACTTCCTGAAAGAAGCGCGTCTGGATCGTGTTGGTTGCTTTAAATACAGCCCGGTTGAAGGCGCAGATGCCAACGCACT
GCCTGACCAGGTGCCGGAAGAAGTCAAAGAAGAACGCTGGAACCGCTTTATGCAGCTGCAACAGCAGATCTCTGCCGAGC
GCCTGCAGGAAAAAGTCGGCAAAGAAATTCTGGTGATTATCGATGAAGTGGATGATGAAGGTGCGATTGGTCGTAGCATG
GCAGACGCCCCTGAAATCGACGGAGCGGTCTATCTCAACGGTGAAAGCAACGTTAAACCTGGCGATATTGTTCGCGTGAA
AGTTGAACATGCCGACGAGTACGATTTGTGGGGAAGTCGGGTTTAA

Upstream 100 bases:

>100_bases
ACGTTGCTGGGCCACGGGGGCAGATAGCGATACAATTTTTGTGCGGAACTCTGTAGAATCCCTGCCCTGACCATTCCTAT
AACTGAATATTTTTTAAGCT

Downstream 100 bases:

>100_bases
TCTCACTGACCCGGCGAGTTGTTCGCCGGGTTTTCCTTTATTATTGCCAGGTAATCTGCGCCCGGCCGTTCTTTTTGCTC
TTATACAGACGTTCATCTGC

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSKVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVT
GCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIGDVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFYNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLL
DFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGKEILVIIDEVDDEGAIGRSM
ADAPEIDGAVYLNGESNVKPGDIVRVKVEHADEYDLWGSRV

Sequences:

>Translated_441_residues
MSKVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVT
GCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
SMRGDLVSRPIGDVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFYNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLL
DFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGKEILVIIDEVDDEGAIGRSM
ADAPEIDGAVYLNGESNVKPGDIVRVKVEHADEYDLWGSRV
>Mature_440_residues
SKVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTG
CLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPS
MRGDLVSRPIGDVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFYNGEPVKTSMVSLCEQLSKLGIWTRLHYVYPY
PHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLD
FLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGKEILVIIDEVDDEGAIGRSMA
DAPEIDGAVYLNGESNVKPGDIVRVKVEHADEYDLWGSRV

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=409, Percent_Identity=26.6503667481663, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=26.1052631578947, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=26.1052631578947, Blast_Score=143, Evalue=3e-34,
Organism=Escherichia coli, GI1787057, Length=441, Percent_Identity=98.4126984126984, Blast_Score=894, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=384, Percent_Identity=28.6458333333333, Blast_Score=152, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=467, Percent_Identity=25.9100642398287, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI71996771, Length=212, Percent_Identity=29.7169811320755, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21356207, Length=475, Percent_Identity=25.8947368421053, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI19922432, Length=408, Percent_Identity=25.7352941176471, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49564; Mature: 49433

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAV
CCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHH
QESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCC
KHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLSEAKR
CCCCCEEECCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHH
LVDAGVKEILVISQDTSAYGVDVKHRTGFYNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
HHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTL
CCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
RSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERW
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
NRFMQLQQQISAERLQEKVGKEILVIIDEVDDEGAIGRSMADAPEIDGAVYLNGESNVKP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GDIVRVKVEHADEYDLWGSRV
CCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SKVTHQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDDADMVIVNTCGFIDSAV
CCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHHH
QESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITGPHSYEQVLEHVHHYVPKP
HHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHCCCC
KHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIGDVLSEAKR
CCCCCEEECCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHHHHHH
LVDAGVKEILVISQDTSAYGVDVKHRTGFYNGEPVKTSMVSLCEQLSKLGIWTRLHYVYP
HHHCCCCEEEEEECCCCCEEEEEHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHEEEEEEC
YPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMKRPGSVDRQLARIKQWREICPELTL
CCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHEE
RSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCFKYSPVEGADANALPDQVPEEVKEERW
CCEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHH
NRFMQLQQQISAERLQEKVGKEILVIIDEVDDEGAIGRSMADAPEIDGAVYLNGESNVKP
HHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCEEEECCCCCCCC
GDIVRVKVEHADEYDLWGSRV
CCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA